REL PDBe PDBe PDBe 2004-08-23 2004-10-06 2004-10-06 2012-10-24 Three-dimensional structure of a bacterial oxalate transporter. Hirai T Heymann JAW Shi D Sarker R Maloney PC Subramaniam S Hirai T Heymann JA Shi D Sarker R Maloney PC Subramaniam S Three-dimensional structure of a bacterial oxalate transporter. NAT.STRUCT.BIOL. 9 597 600 2002 12118242 doi:10.1038/nsb821 Oxalate Transporter Oxalate Transporter 1 oxalate transporter Oxalobacter formigenes cell membrane 0.044
Oxalate(-ooc-coo-) is believed to be bound in the central cavity of OxlT in this crystal form. Endogenous E.coli lipids and added synthetic lipids are expected to be present in this two dimensional crystal. No detectable density for lipid or oxalate is observed at current resolution.
true Escherichia coli pBluescript II SKplus and pMS421 electronCrystallography twoDArray
400 mesh Cu grid
 NITROGEN 77 HOMEMADE PLUNGER
Vitrification instrument: MRC plunger
 FEI TECNAI F30 SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.2 0.1 2.0 59000.0 57000.0 GATAN LIQUID NITROGEN 90 95 astigmatism was corrected at 200,000 times magnification
Tecnai12, 120KV with tungsten filament was also used at early stage. Floodbeam was also used for some images as illumination mode.
KODAK SO-163 FILM ZEISS SCAI 7 47 10
The best images were selected by optical diffraction.
0.5 10. side entry 0 45 0 45
Specimens were either embedded in 3.5% trehalose or prepared as frozen-hydrated specimens.
6.5 OTHER MRC
Amplitudes were scaled with respect to reference amplitudes from the helix model.
 100.3 79 100 90 90 90 P 2 21 21
ctfsearch or ttrefine on each image
 emd_1098.map.gz 18 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 86 65 157 81 14 -78 150 182 182 100.30002 79.00005 100.000084 90.0 90.0 90.0 X Y Z -162.380218509999992 256.0 5.49867582 48.494132999999998 0.551099 0.526667 0.549451 54.0 AUTHOR This is an image of the bacterial oxalate transporter(OxlT)in the oxalate-bound, "closed" state. OxlT is a representative member of the Major Facilitator Superfamily (MFS).
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