REL PDBe PDBe PDBe 2004-09-13 2004-09-23 2006-09-23 2011-05-26 Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Beck M Forster F Ecke M Plitzko JM Melchior F Gerisch G Baumeister W Medalia O Beck M Forster F Ecke M Plitzko JM Melchior F Gerisch G Baumeister W Medalia O Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. SCIENCE 306 1387 1390 2004 15514115 doi:10.1126/science.1104808 nuclear pore complex nuclear pore complex 1 nuclear pore complex Dictyostelium discoideum AX2 Nucleus Nucleus false subtomogramAveraging particle
50 mM Tris pH 7.6, 25 mM KCl, 5mM MgCl2, 50 mM sucrose
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: Rudolf Gatz plunger
 FEI/PHILIPS CM300FEG/T FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.0 17500.0 GATAN LIQUID NITROGEN 90 GIF2000 0.0 20.0 GATAN MULTISCAN Eucentric 63
Average number of projections used in the 3D reconstructions: 267.
OTHER 85.0 FSC 0.5 CUT-OFF TOM
3D reconstruction by averaging of subtomograms
 emd_1097.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 2099.2 2099.2 2099.2 90 90 90 X Y Z -3.05764 3.81137 0.0933206 0.55606 16.4 16.4 16.4 1.22 3D structure of the Dictyostelium nuclear pore complex obtained by Cryoelectron Tomography
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