REL PDBe PDBe PDBe 2004-09-01 2004-09-01 2005-09-01 2011-05-26 A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation. Sewell BT Best RB Chen S Roseman AM Farr GW Horwich AR Saibil HR Sewell BT Best RB Chen S Roseman AM Farr GW Horwich AL Saibil HR A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation. NAT.STRUCT.MOL.BIOL. 11 1128 1133 2004 15475965 doi:10.1038/nsmb844 E461K mutant of GroEL E461K mutant of GroEL homo tetradecamer 1 0.84 0.84 sequencing, gel-filtration E461K mutant of GroEL Escherichia coli E. coli 0.84 0.84
homo tetradecamer
14 homo tetradecamer true Escherichia coli singleParticle particle 0.84 7.4
20 mM Tris, 5 mM KCl, 10 mM MgCl2, pH 7.4
 NEGATIVE
grids were flash frozen by plunging into liquid ethane at -170 degrees C. No stain was used.
copper grid, holey carbon films
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: Home made plunger
grids were blotted prior to plunging for approximately two seconds JEOL 2010HT FLOOD BEAM BRIGHT FIELD TUNGSTEN HAIRPIN 200 1.0 2.0 30000.0 30000.0 OTHER 100 100 100 KODAK SO-163 FILM OTHER 10 7 10
Scanned on a Leafscan 45 at 2540 pixels per inch. Subsequently pixels were averaged in 2x2 blocks.
16. Oxford CT3500
Side views only were used in the reconstruction
C7 OTHER 25.0 FSC 0.5 CUT-OFF Spider
Seven fold symmetry was imposed on the reconstruction.
1477
Spider euler angles file as follows: 1 3 90.0 90.000 0.00000E+00 2 3 90.0 90.000 3.2143 3 3 90.0 90.000 6.4286 4 3 90.0 90.000 9.6429 5 3 90.0 90.000 12.857 6 3 90.0 90.000 16.071 7 3 90.0 90.000 19.286 8 3 90.0 90.000 22.500 9 3 90.0 90.000 25.714 10 3 90.0 90.000 28.929 11 3 90.0 90.000 32.143 12 3 90.0 90.000 35.357 13 3 90.0 90.000 38.572 14 3 90.0 90.000 41.786 15 3 90.0 90.000 45.000 16 3 90.0 90.000 48.214 17 3 90.0 96.429 0.00000E+00 18 3 90.0 96.429 3.2143 19 3 90.0 96.429 6.4286 20 3 90.0 96.429 9.6429 21 3 90.0 96.429 12.857 22 3 90.0 96.429 16.071 23 3 90.0 96.429 19.286 24 3 90.0 96.429 22.500 25 3 90.0 96.429 25.714 26 3 90.0 96.429 28.929 27 3 90.0 96.429 32.143 28 3 90.0 96.429 35.357 29 3 90.0 96.429 38.572 30 3 90.0 96.429 41.786 31 3 90.0 96.429 45.000 32 3 90.0 96.429 48.214 33 3 90.0 102.857 0.0 34 3 90.0 102.857 6.4825 35 3 90.0 102.857 12.857 36 3 90.0 102.857 19.2885 37 3 90.0 102.857 25.714 38 3 90.0 102.857 32.1425 39 3 90.0 102.857 38.571 40 3 90.0 102.857 45.0
 40 emd_1095.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 426.88 426.88 426.88 90 90 90 X Y Z -0.0751354 0.084824 -0.000167728 0.00857093 6.67 6.67 6.67 0.017 Map of the E461K mutant of GroEL made from 1477 images picked from 7 cryo micrographs. Big-endian bypte order.
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 1EMS RIGID BODY FIT
The seven membered rings of GroEL (1EMS) were docked separately into the reconstructed density. This was done manually using the programme O.