REL PDBe PDBe PDBe 2004-05-26 2004-08-24 2004-09-24 2012-10-17 Electron microscopic analysis of KvAP voltage-dependent K+ channels in an open conformation. Jiang QX Wang DN MacKinnon R Jiang QX Wang DN MacKinnon R Electron microscopic analysis of KvAP voltage-dependent K+ channels in an open conformation. NATURE 430 806 810 2004 15306816 doi:10.1038/nature02735 KvAP complexed with 33H1 Fab fragments KvAP complexed with 33H1 Fab fragments
none
one 2 0.34 0.3 the sequence and the estimate of detergent micelle size KvAP potassium channel Aeropyrum pernix A. pernix plasma membrane 0.12 0.12
one channel
1 tetramer true Escherichia coli pQE60 33H1 Fab fragments Mus musculus House Mouse 0.18 0.18
four Fab fragments
4 monomer false singleParticle particle 5.0 7.4
40 mM KCl, 60 mM NaCl, 20 mM Tris-HCl pH 7.4, 5.0 mM DM
 NEGATIVE
mix with equal volume of 65% ammonium molybdate
400 mesh grids with holey carbon film
 ETHANE 60 298 HOMEMADE PLUNGER
Vitrification instrument: home-made
8 FEI/PHILIPS CM200T FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 0.5 1.1 50000.0 OTHER 100 corrected at 200000 KODAK SO-163 FILM ZEISS SCAI 14 37 20 1.2 8. Oxford 0 0
the holes in the carbon film were covered with a thin-layer of carbon film.
each particle
 C4 OTHER 10.5 FSC 0.5 CUT-OFF IMAGIC SPIDER 21379 496 emd_1094.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 60 60 60 -30 -30 -30 60 60 60 168 168 168 90 90 90 X Y Z -10.7372 8.15827 0.0763837 1.00394 2.8 2.8 2.8 2.12 A map of the KvAP/33H1Fab complex at 10.5 angstrom resolution. A threshold at 1.0 gives 340 kDa.
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