Cryo-EM structure of T7 bacteriophage DNA translocation gp15-gp16 core complex intermediate assembly

2024-07-10 REL PDBe PDBe PDBe 2020-04-23 2021-09-08 2021-09-08 2024-07-10 Spanish Ministry of Science, Innovation, and Universities BFU 2014-54181 Spain Spanish Ministry of Science, Innovation, and Universities SEV-2013-0347 Spain Spanish Ministry of Science, Innovation, and Universities BES-2015-073615 Spain Cryo-EM structure of T7 bacteriophage DNA translocation gp15-gp16 core complex intermediate assembly Perez-Ruiz M Pulido-Cid M VIRAL COMPLEX, DNA TRANSLOCATION, VIRAL INFECTION, TRANS-GLYCOSYLASE ACTIVITY, CHAPERONE, PERIPLASMIC SPACE COMPLEX, VIRAL PROTEIN Perez-Ruiz M Pulido-Cid M Luque-Ortega JR Valpuesta JM Cuervo A Carrascosa JL Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope. Proc.Natl.Acad.Sci.USA US 118 2021 34417311 doi:10.1073/pnas.2026719118 1091-6490 0040 PNASA6 EMD-10911 other EM volume 6yt5 FULLOVERLAP gp15-gp16 core complex gp15-gp16 core complex 0 1 2 Escherichia phage T7 1.4 Internal virion protein gp15 Escherichia phage T7 0.08837721899999999 6 Escherichia coli BL21(DE3) LEVO MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSMSKIESALQAAQPGLSRLRGGAGGMGYRAATTQAEQPRSSLLDTI GRFAKAGADMYTAKEQRARDLADERSNEIIRKLTPEQRREALNNGTLLYQDDPYAMEALRVKTGRNAAYLVDDDVMQKIK EGVFRTREEMEEYRHSRLQEGAKVYAEQFGIDPEDVDYQRGFNGDITERNISLYGAHDNFLSQQAQKGAIMNSRVELNGV LQDPDMLRRPDSADFFEKYIDNGLVTGAIPSDAQATQLISQAFSDASSRAGGADFLMRVGDKKVTLNGATTTYRELIGEE QWNALMVTAQRSQFETDAKLNEQYRLKINSALNQEDPRTAWEMLQGIKAELDKVQPDEQMTPQREWLISAQEQVQNQMNA WTKAQAKALDDSMKSMNKLDVIDKQFQKRINGEWVSTDFKDMPVNENTGEFKHSDMVNYANKKLAEIDSMDIPDGAKDAM KLKYLQADSKDGAFRTAIGTMVTDAGQEWSAAVINGKLPERTPAMDALRRIRNADPQLIAALYPDQAELFLTMDMMDKQG IDPQVILDADRLTVKRSKEQRFEDDKAFESALNASKAPEIARMPASLRESARKIYDSVKYRSGNESMAMEQMTKFLKEST YTFTGDDVDGDTVGVIPKNMMQVNSDPKSWEQGRDILEEARKGIIASNPWITNKQLTMYSQGDSIYLMDTTGQVRVRYDK ELLSKVWSENQKKLEEKAREKALADVNKRAPIVAATKAREAAAKRVREKRKQTPKFIYGRKE P03725 Peptidoglycan transglycosylase gp16 Escherichia phage T7 0.146693094 6 Escherichia coli BL21(DE3) LEVO MGHHHHHHHHHHSSGHIEGRHMMDKYDKNVPSDYDGLFQKAADANGVSYDLLRKVAWTESRFVPTAKSKTGPLGMMQFTK ATAKALGLRVTDGPDDDRLNPELAINAAAKQLAGLVGKFDGDELKAALAYNQGEGRLGNPQLEAYSKGDFASISEEGRNY MRNLLDVAKSPMAGQLETFGGITPKGKGIPAEVGLAGIGHKQKVTQELPESTSFDVKGIEQEATAKPFAKDFWETHGETL DEYNSRSTFFGFKNAAEAELSNSVAGMAFRAGRLDNGFDVFKDTITPTRWNSHIWTPEELEKIRTEVKNPAYINVVTGGS PENLDDLIKLANENFENDSRAAEAGLGAKLSAGIIGAGVDPLSYVPMVGVTGKGFKLINKALVVGAESAALNVASEGLRT SVAGGDADYAGAALGGFVFGAGMSAISDAVAAGLKRSKPEAEFDNEFIGPMMRLEARETARNANSADLSRMNTENMKFEG EHNGVPYEDLPTERGAVVLHDGSVLSASNPINPKTLKEFSEVDPEKAARGIKLAGFTEIGLKTLGSDDADIRRVAIDLVR SPTGMQSGASGKFGATASDIHERLHGTDQRTYNDLYKAMSDAMKDPEFSTGGAKMSREETRYTIYRRAALAIERPELQKA LTPSERIVMDIIKRHFDTKRELMENPAIFGNTKAVSIFPESRHKGTYVPHVYDRHAKALMIQRYGAEGLQEGIARSWMNS YVSRPEVKARVDEMLKELHGVKEVTPEMVEKYAMDKAYGISHSDQFTNSSIIEENIEGLVGIENNSFLEARNLFDSDLSI TMPDGQQFSVNDLRDFDMFRIMPAYDRRVNGDIAIMGSTGKTTKELKDEILALKAKAEGDGKKTGEVHALMDTVKILTGR ARRNQDTVWETSLRAINDLGFFAKNAYMGAQNITEIAGMIVTGNVRALGHGIPILRDTLYKSKPVSAKELKELHASLFGK EVDQLIRPKRADIVQRLREATDTGPAVANIVGTLKYSTQELAARSPWTKLLNGTTNYLLDAARQGMLGDVISATLTGKTT RWEKEGFLRGASVTPEQMAGIKSLIKEHMVRGEDGKFTVKDKQAFSMDPRAMDLWRLADKVADEAMLRPHKVSLQDSHAF GALGKMVMQFKSFTIKSLNSKFLRTFYDGYKNNRAIDAALSIITSMGLAGGFYAMAAHVKAYALPKEKRKEYLERALDPT MIAHAALSRSSQLGAPLAMVDLVGGVLGFESSKMARSTILPKDTVKERDPNKPYTSREVMGAMGSNLLEQMPSAGFVANV GATLMNAAGVVNSPNKATEQDFMTGLMNSTKELVPNDPLTQQLVLKIYEANGVNLRERRK P03726 4.2.2.- singleParticle particle 1.5 8.0 100.0 Tris 100.0 NaCitrate 10.0 MgCl2 Quantifoil R2/2 GLOW DISCHARGE 60 AIR ETHANE 95 293.15 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 47619.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 2 5506 8.0 40.8 1 1 C6 FOURIER SPACE 3.0 FSC 0.143 CUT-OFF RELION 3.0 72882 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION RELION 3.0 emd_10912.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 335.04 335.04 335.04 90.0 90.0 90.0 X Y Z -0.011678981 0.15056488 0.00009248733 0.0035224585 1.047 1.047 1.047 0.006 AUTHOR ::::EMDATABANK.org::::EMD-10912:::: t7 tail complex 1SLY A PDB experimental model 4CFO A PDB experimental model AB INITIO MODEL REAL