<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_9_3/emdb.xsd" version="3.0.9.3" emdb_id="EMD-10890">
    <admin>
        <current_status>
            <date>2024-05-22</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-04-20</deposition>
            <header_release>2020-06-24</header_release>
            <map_release>2020-06-24</map_release>
            <update>2024-05-22</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>German Research Foundation (DFG)</funding_body>
                <code>FR1653/6-1</code>
                <country>Germany</country>
            </grant_reference>
            <grant_reference>
                <funding_body>German Research Foundation (DFG)</funding_body>
                <code>SFB902</code>
                <country>Germany</country>
            </grant_reference>
        </grant_support>
        <title>cryo-electron density map of the P140-P110 heterodimer</title>
        <authors_list>
            <author>Scheffer MP</author>
            <author>Aparicio D</author>
        </authors_list>
        <keywords>P110/P140 Nap Mycoplasma genitalium surface adhesin, CELL ADHESION</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Aparicio D</author>
                    <author order="2">Scheffer MP</author>
                    <author order="3">Marcos-Silva M</author>
                    <author order="4">Vizarraga D</author>
                    <author order="5">Sprankel L</author>
                    <author ORCID="0000-0001-5224-9172" order="6">Ratera M</author>
                    <author ORCID="0000-0003-2125-8222" order="7">Weber MS</author>
                    <author order="8">Seybert A</author>
                    <author order="9">Torres-Puig S</author>
                    <author ORCID="0000-0002-5853-967X" order="10">Gonzalez-Gonzalez L</author>
                    <author order="11">Reitz J</author>
                    <author order="12">Querol E</author>
                    <author order="13">Pinol J</author>
                    <author ORCID="0000-0003-3561-630X" order="14">Pich OQ</author>
                    <author order="15">Fita I</author>
                    <author ORCID="0000-0002-8067-6611" order="16">Frangakis AS</author>
                    <title>Structure and mechanism of the Nap adhesion complex from the human pathogen Mycoplasma genitalium.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>11</volume>
                    <first_page>2877</first_page>
                    <last_page>2877</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32513917</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-020-16511-2</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-10260</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10259</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6yrk</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>P140-P110 heterodimer</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>P140-P110 heterodimer</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="243273">Mycoplasma genitalium G37</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Mgp-operon protein 3</name>
                <natural_source database="NCBI">
                    <organism ncbi="243273">Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.08481696899999999</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="243273">Mycoplasma genitalium G37</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>TFLVKEDSKNVTAYTPFATPITDSKSDLVSLAQLDSSYQIADQTIHNTNLFVLFKSRDVKVKYESSGSNNISFDSTSQGE
KPSYVVEFTNSTGIKWTMVKKYQLDVPNVSSDMNQVLKNLILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQANQWTSQR
NQHDLNNNPSPNASTGFKLTTGNAYRKLSESWPIYEPIDGTKQGKGKDSSGWSSTEENEAKNDAPSVSSGTFNKYLNTKQ
ALESIGILFDDQTPRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSMWYWVVERSAQENASNKPTWFANTNLDWGEDKQK
QFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSTKDQALAWSTTTSLDSK
TGYKDLVTNDTGLNGPINGSFSIQDTFSFVVPYSGNHTNNGTTGPIKTAYPVKKDQKSTVKINSLINATPLNSYGDEGIG
VFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVNWSNTQSRYLPVPYNYSEGIIDADGFKR
PENRGASVTTFSGLKSIAPDGFANSIANFSVGLKAGIDPNPVMSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNNI
APISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYIFDSSLKPSHDGKQTPVTDNMGFSVITVSRTGIELNQDQATTTL
DVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKDSDQNKIDIYKNNNGLFEIDT</string>
                    <external_references type="UNIPROTKB">P22747</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Adhesin P1</name>
                <natural_source database="NCBI">
                    <organism ncbi="243273">Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.128447648</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="243273">Mycoplasma genitalium G37</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>QAVDETLTPWTWNNNNFSSLKITGENPGSFGLVRSQNDNLNISSVTKDNLKYLNAVEKYLDGQQNFAIRRYDNNGRALYD
INLAKMENPSTVQRGLNGEPIFDPFKGFGLTGNAPTDWNEIKGKVPVEVVQSPHSPNLYFVLLVPKVALEYHNLNNQVVK
ESLEVSSFNPTQRLQKDSPVKDSSKQGEKLSETTASSMSSGMATSTRAKALKVEVERGSQSDSLLKNDFAKKPLKHKNSS
GEVKLEAEKEFTEAWKPLLTTDQIAREKGMGATVVSFYDAPYSENHTAFGLVDHIDPKKMVENYPPSWKTPKWNHHGIWD
YNARNLLLQTTGFFNPRRHPEWFDEGQAKADNTSPGFKVGDTDHKKDGFKKNSSSPIALPFEAYFANIGNMVAIGNSVFI
FGGNGHATKMFTTNPLSIGVFRIKYTDNFSKSSVTGWPYAVLFGGLINPQTNGLKDLPLGTNRWFEYVPRMAVSGVKWVG
NQLVLAGTLTMGDTATVPRLKYDQLEKHLNLVAQGQGLLREDLQIFTPYGWANRPDIPVGAWLQDEMGSKFGPHYFLNNP
DIQDNVNNDTVEALISSYKNTDKLKHVYPYRYSGLYAWQLFNWSNKLTNTPLSANFVNENSYAPNSLFAAILNEDLLTGL
SDKIFYGKENEFAENEADRFNQLLSLNPNPNTNWARYLNVVQRFTTGPNLDSSTFDQFLDFLPWIGNGKPFSNSPSASTP
LPTFSNINVGVKSMITQHLNKENTRWVFIPNFSPDIWTGAGYRVQSANQKNGIPFEQVKPSNNSTPFDPNSDDNKVTPSG
GSSKPTTYPALPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGDSNDQFNKDSEQKWDKTETNEGNLP
GFGEVNGLYNAALLHTYGFFGTNTNSTDPKIGFKADSSSSSSSTLVGSGLNWTSQDVGNLVVINDTSFGFQLGGWFITFT
DFIRPRTGYLGITLSSLQDQTIIWADQPWTSFKGSYLDSDGTPKSLWDPTALKSLPNNPTLSPSFQLYQPNKVKAYQTTN
TYNKLIEPVDATSAATNMTSLLKLLTTKNIKAKLGKGTASNNGGGVSQTINTITTTGNISEGLKEETSIQAETLKKFFDS
KQNNKSEIGIGDSTFTKMDGKLTGVVSTPLVNLINGQGAT</string>
                    <external_references type="UNIPROTKB">P20796</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.6</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>SUPER-RESOLUTION</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">7.5</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="INSILICO MODEL"/>
                <final_reconstruction>
                    <number_classes_used>6</number_classes_used>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">4.1</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                    <details>Resolution is anisotropic</details>
                    <number_images_used>37009</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="78733">
        <file>emd_10890.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>270</col>
            <row>270</row>
            <sec>270</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>270</x>
            <y>270</y>
            <z>270</z>
        </spacing>
        <cell>
            <a units="Å">283.5</a>
            <b units="Å">283.5</b>
            <c units="Å">283.5</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.012912566</minimum>
            <maximum>0.033811565</maximum>
            <average>0.00014913664</average>
            <std>0.0014534903</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.05</x>
            <y units="Å">1.05</y>
            <z units="Å">1.05</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.01</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-10890::::</label>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <initial_model>
                    <access_code>6RUT</access_code>
                    <chain>
                        <residue_range>59-1243</residue_range>
                        <source_name>PDB</source_name>
                        <initial_model_type>experimental model</initial_model_type>
                    </chain>
                </initial_model>
                <initial_model>
                    <access_code>6R3T</access_code>
                    <chain>
                        <residue_range>25-936</residue_range>
                        <source_name>PDB</source_name>
                        <initial_model_type>experimental model</initial_model_type>
                    </chain>
                </initial_model>
                <refinement_protocol>RIGID BODY FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
    </interpretation>
</emd>
