REL PDBe PDBe PDBe 2004-06-09 2004-06-10 2004-06-10 2013-10-02 Three-dimensional structure of the bacterial multidrug transporter EmrE shows it is an asymmetric homodimer. Ubarretxena-Belandia I Baldwin JM Schuldiner S Tate CG Ubarretxena-Belandia I Baldwin JM Schuldiner S Tate CG Three-dimensional structure of the bacterial multidrug transporter EmrE shows it is an asymmetric homodimer. EMBO J. 22 6175 6181 2003 14633977 doi:10.1093/emboj/cdg611 2i68 FULLOVERLAP EmrE from E. coli containing the bound substrate tetraphenylphosphonium EmrE from E. coli containing the bound substrate tetraphenylphosphonium
EmrE in the 2D crystals is in a functional state with the substrate (TPP+) bound in a binding pocket formed from 6 out of the 8 helices in the asymmetric dimer.
Asymmetric homodimer 1 0.0152 EmrE Escherichia coli K12 Inner membrane 0.0152 0.0152
4-helix integral membrane protein; The engineered EmrE is fully functional as assessed by in vivo and in vitro transport assays
2 dimer true Escherichia coli pT7-7 P23895 GO:0016021 IPR000390 electronCrystallography twoDArray 1 7.5
20 mM Sodium phosphate pH7.5, 100 mM NaCl, 2mM MgCl2, 1mM EDTA, 1 mM Na azide, 10uM tetraphenylphosphonium
 NEGATIVE
Crystals on carbon support were washed with 1% glucose pH 8 (aq ammonia) containing 25ug/ml bacitracin, 2 times 20ul, and blotted to dryness
300 mesh molybdenum grid
 NITROGEN 45 77 HOMEMADE PLUNGER
Vitrification instrument: manual. Performed at room temperature
Blot to dryness for 10 seconds before freezing
2D crystals were grown in suspension by dialysis
2D crystals were grown in suspension by dialysis
 FEI TECNAI F30 SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.3 0.2 1.6 60000.0 57400.0 GATAN LIQUID NITROGEN 77 77 77 objective lens astigmatism was corrected at 200,000 times magnification KODAK SO-163 FILM ZEISS SCAI 7 47 15 Side entry liquid nitrogen-cooled cryo specimen holder 0 46 0 46 OTHER 7.5 OTHER MRC
Resolution in the membrane plane is 7.5 angstroms and 16 angstroms perpendicular to the membrane plane
 72.1 86.8 107.3 P 2
CTFFIND2 on each image
 emd_1087.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 81 89 21 -2 -63 -10 150 150 80 72.10005 86.80005 200.0 90.0 90.0 107.3 Y X Z -156.908599850000002 250.000015259999998 -2.69374633 47.366405489999998 0.48066702 0.578667 2.5 59.700000000000003 AUTHOR Density map of the asymmetric dimer of EmrE,a multidrug transporter from Escherichia coli
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