<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" emdb_id="EMD-1086" version="3.0.1.1" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_1/emdb_relaxed.xsd">
   <admin>
      <current_status>
         <code>REL</code>
         <processing_site>PDBe</processing_site>
      </current_status>
      <sites>
         <deposition>PDBe</deposition>
         <last_processing>PDBe</last_processing>
      </sites>
      <key_dates>
         <deposition>2004-06-03</deposition>
         <header_release>2004-06-03</header_release>
         <map_release>2004-09-07</map_release>
         <update>2013-04-17</update>
      </key_dates>
      <title>Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host.</title>
      <authors_list>
         <author>Leiman PG</author>
         <author>Chipman PR</author>
         <author>Kostyuchenko VA</author>
         <author>Mesyanzhinov VV</author>
         <author>Rossmann MG</author>
      </authors_list>
      <keywords>
         </keywords>
   </admin>
   <crossreferences>
      <citation_list>
         <primary_citation>
            <journal_citation published="true">
               <author order="1">Leiman PG</author>
               <author order="2">Chipman PR</author>
               <author order="3">Kostyuchenko VA</author>
               <author order="4">Mesyanzhinov VV</author>
               <author order="5">Rossmann MG</author>
               <title>Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host.</title>
               <journal>CELL(CAMBRIDGE,MASS.)</journal>
               <volume>118</volume>
               <first_page>419</first_page>
               <last_page>429</last_page>
               <year>2004</year>
               <external_references type="PUBMED">15315755</external_references>
               <external_references type="DOI">doi:10.1016/j.cell.2004.07.022</external_references>
            </journal_citation>
         </primary_citation>
      </citation_list>
      <pdb_list>
         <pdb_reference>
            <pdb_id>1tja</pdb_id>
            <relationship>
               <in_frame>FULLOVERLAP</in_frame>
            </relationship>
         </pdb_reference>
         <pdb_reference>
            <pdb_id>3j2n</pdb_id>
            <relationship>
               <in_frame>FULLOVERLAP</in_frame>
            </relationship>
         </pdb_reference>
         <pdb_reference>
            <pdb_id>2fl9</pdb_id>
            <relationship>
               <in_frame>FULLOVERLAP</in_frame>
            </relationship>
         </pdb_reference>
         <pdb_reference>
            <pdb_id>3foi</pdb_id>
            <relationship>
               <in_frame>FULLOVERLAP</in_frame>
            </relationship>
         </pdb_reference>
         <pdb_reference>
            <pdb_id>3h3y</pdb_id>
            <relationship>
               <in_frame>FULLOVERLAP</in_frame>
            </relationship>
         </pdb_reference>
      </pdb_list>
   </crossreferences>
   <sample>
      <name>T4 phages treated with 3 M urea</name>
      <supramolecule_list>
         <sample_supramolecule supramolecule_id="1000">
            <name>T4 phages treated with 3 M urea</name>
            <number_unique_components>1</number_unique_components>
            <molecular_weight>
               <experimental units="MDa">220</experimental>
               <theoretical units="MDa">220</theoretical>
               <method>Estimate</method>
            </molecular_weight>
         </sample_supramolecule>
         <virus_supramolecule supramolecule_id="1">
            <name synonym="phage T4">Enterobacteria phage T4</name>
            <details>treated with 3 M urea</details>
            <sci_species_name ncbi="10665">Enterobacteria phage T4</sci_species_name>
            <natural_host database="NCBI">
               <organism ncbi="562">Escherichia coli</organism>
               <synonym_organism>BACTERIA(EUBACTERIA)</synonym_organism>
            </natural_host>
            <host_system database="NCBI">
               </host_system>
            <molecular_weight>
               <experimental units="MDa">220</experimental>
               <theoretical units="MDa">220</theoretical>
            </molecular_weight>
            <virus_type>VIRION</virus_type>
            <virus_isolate>STRAIN</virus_isolate>
            <virus_enveloped>false</virus_enveloped>
            <virus_empty>false</virus_empty>
            <syn_species_name>phage T4</syn_species_name>
         </virus_supramolecule>
      </supramolecule_list>
   </sample>
   <structure_determination_list>
      <structure_determination structure_determination_id="1">
         <method>singleParticle</method>
         <aggregation_state>particle</aggregation_state>
         <specimen_preparation_list>
            <single_particle_preparation preparation_id="1">
               <concentration units="mg/mL">5</concentration>
               <buffer>
                  <ph>7.5</ph>
                  <details>H2O</details>
               </buffer>
               <grid>
                  <details>200 mesh cupper grid</details>
               </grid>
               <vitrification>
                  <cryogen_name>ETHANE</cryogen_name>
                  <chamber_temperature units="K">100</chamber_temperature>
               </vitrification>
            </single_particle_preparation>
         </specimen_preparation_list>
         <microscopy_list>
            <single_particle_microscopy microscopy_id="1">
               <microscope>FEI/PHILIPS CM300FEG/T</microscope>
               <illumination_mode>SPOT SCAN</illumination_mode>
               <imaging_mode>BRIGHT FIELD</imaging_mode>
               <electron_source>FIELD EMISSION GUN</electron_source>
               <acceleration_voltage units="kV">300</acceleration_voltage>
               <nominal_defocus_min units="&#181;m">0.5</nominal_defocus_min>
               <nominal_defocus_max units="&#181;m">3.4</nominal_defocus_max>
               <nominal_magnification>45000.0</nominal_magnification>
               <calibrated_magnification>47000.0</calibrated_magnification>
               <specimen_holder_model>GATAN LIQUID NITROGEN</specimen_holder_model>
               <temperature>
                  <temperature_average units="K">100</temperature_average>
               </temperature>
               <specialist_optics>
                  <energy_filter>
                     <name>FEI</name>
                  </energy_filter>
               </specialist_optics>
               <date>2002-01-06</date>
               <image_recording_list>
                  <image_recording>
                     <film_or_detector_model category="FILM">KODAK SO-163 FILM</film_or_detector_model>
                     <digitization_details>
                        <scanner>ZEISS SCAI</scanner>
                        <sampling_interval units="&#181;m">3.93285</sampling_interval>
                     </digitization_details>
                     <number_real_images>100</number_real_images>
                     <average_electron_dose_per_image units="e/&#8491;^2">20</average_electron_dose_per_image>
                     <bits_per_pixel>8.</bits_per_pixel>
                  </image_recording>
               </image_recording_list>
               <specimen_holder>Side entry liquid nitrogen-cooled cryo specimen holder</specimen_holder>
               <tilt_angle_min>0</tilt_angle_min>
               <tilt_angle_max>0</tilt_angle_max>
            </single_particle_microscopy>
         </microscopy_list>
         <singleparticle_processing image_processing_id="1">
            <ctf_correction>
               <details>Each particle</details>
            </ctf_correction>
            <final_reconstruction>
               <applied_symmetry>
                  <point_group>C6</point_group>
               </applied_symmetry>
               <algorithm>OTHER</algorithm>
               <resolution res_type="BY AUTHOR" units="&#8491;">16.0</resolution>
               <resolution_method>FSC 0.5 CUT-OFF</resolution_method>
               <software_list>
                  <software>
                     <name>Spider</name>
                  </software>
               </software_list>
               <number_images_used>1965</number_images_used>
            </final_reconstruction>
            <final_angle_assignment>
               <details>theta 45 degrees, phi 180 degrees</details>
            </final_angle_assignment>
         </singleparticle_processing>
      </structure_determination>
   </structure_determination_list>
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      <file>emd_1086.map.gz</file>
      <symmetry>
         <space_group>1</space_group>
      </symmetry>
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         <col>256</col>
         <row>256</row>
         <sec>256</sec>
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      <origin>
         <col>-128</col>
         <row>-128</row>
         <sec>-127</sec>
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      <spacing>
         <x>256</x>
         <y>256</y>
         <z>256</z>
      </spacing>
      <cell>
         <a units="&#8491;">1006.8096</a>
         <b units="&#8491;">1006.8096</b>
         <c units="&#8491;">1006.8096</c>
         <alpha units="deg">90.0</alpha>
         <beta units="deg">90.0</beta>
         <gamma units="deg">90.0</gamma>
      </cell>
      <axis_order>
         <fast>X</fast>
         <medium>Y</medium>
         <slow>Z</slow>
      </axis_order>
      <statistics>
         <minimum>-11.223052020000001</minimum>
         <maximum>14.634929659999999</maximum>
         <average>0.0</average>
         <std>1.0</std>
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         <x units="&#8491;">3.93285</x>
         <y units="&#8491;">3.93285</y>
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      </pixel_spacing>
      <contour_list>
         <contour primary="true">
            <level>1.5</level>
            <source>AUTHOR</source>
         </contour>
      </contour_list>
      <annotation_details>CryoEM density of the T4 tail with contracted sheath and the baseplate in the star conformation.</annotation_details>
      <details>::::EMDATABANK.org::::EMD-1086::::</details>
   </map>
   <interpretation>
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         <modelling>
            <initial_model>
               <access_code>1N7Z</access_code>
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            <software_list>
               <software>
                  <name>Situs v.2.0</name>
               </software>
            </software_list>
            <details>The components were separately fitted using Situs v.2.0</details>
            <refinement_space>REAL</refinement_space>
         </modelling>
         <modelling>
            <initial_model>
               <access_code>1S2E</access_code>
            </initial_model>
            <software_list>
               <software>
                  <name>Situs v.2.0</name>
               </software>
            </software_list>
            <details>The components were separately fitted using Situs v.2.0</details>
            <refinement_space>REAL</refinement_space>
         </modelling>
         <modelling>
            <initial_model>
               <access_code>1EL6</access_code>
            </initial_model>
            <software_list>
               <software>
                  <name>Situs v.2.0</name>
               </software>
            </software_list>
            <details>The components were separately fitted using Situs v.2.0</details>
            <refinement_space>REAL</refinement_space>
         </modelling>
      </modelling_list>
   </interpretation>
</emd>