2020-07-15
REL
PDBe
PDBe
PDBe
2020-04-08
2020-06-17
2020-06-17
2020-07-15
European Research Council (ERC)
ERC-2015-CoG-681582
United Kingdom
Medical Research Council (MRC, United Kingdom)
MC_PC_17135
United Kingdom
Mono-ubiquitinated FANCD2 in complex with FANCI
Rennie ML
Lemonidis K
Arkinson C
Chaugule VK
Clarke M
Streetley J
Spagnolo L
Walden H
Rennie ML
Lemonidis K
Arkinson C
Chaugule VK
Clarke M
Streetley J
Spagnolo L
Walden H
Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA.
Embo Rep.
UK
21
e50133
e50133
2020
32510829
doi:10.15252/embr.202050133
1469-3178
EMD-10844
other EM volume
Same complex with mono-ubiquitinated FANCI, and dsDNA
EMD-10843
associated EM volume
Binary complex of mono-ubiquitinated FANCD2 and FANCI
Binary complex of mono-ubiquitinated FANCD2 and FANCI
0
1
2
Homo sapiens
Spodoptera frugiperda
0.33
FANCD2 mono-ubiquitinated at K561
Homo sapiens
Additional single ubiquitin conjugated to K561 (ubiquitin sequence: GPGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG)
Spodoptera frugiperda
LEVO
GPGSMVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLL
KISGIILKTGESQNQLAVDQIAFQKKLFQTL RRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDE
EASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWL DRVVDGKDLTTKI
MQLISIAPENLQHDIITSLPEILGDSQHADVGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMD
KLSSIRLEDLPVIIK FILHSVTAMDTLEVISELREKLDLQHCVLPSRLQASQVKLKSKGRASSSGNQESS
GQSCIILLFDVIKSAIRYEKTISEAWIKAIENTASVSEHKVFDLV MLFIIYSTNTQTKKYIDRVLRNKIRSGCI
QEQLLQSTFSVHYLVLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGALVTHICS
GNEAEVDTALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISPQQIRKLFYVLSTLAFSKQNEASSHIQD
DMHLVIRKQLSSTVFKYKLIGIIGAVTMAG IMAADRSESPSLTQERANLSDEQCTQVTSLLQLVHSCSE
QSPQASALYYDEFANLIQHEKLDPKALEWVGHTICNDFQDAFVVDSCVVPEGDFPFPVKAL YGLEEYD
TQDGIAINLLPLLFSQDFAKDGGPVTSQESGQKLVSPLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPI
FLTDLEPGEKLESMSAKERSFMC SLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIILEKYLA
VTPDYVPPLGNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDGSKTSSSD TLSEEKNSECDPTPSHRG
QLNKEFTGKEEKTSLLLHNSHAFFRELDIEVFSILHCGLVTKFILDTEMHTEATEVVQLGPPELLFLLEDL
SQKLESMLTPP IARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLLTPMCNHLENIHNYFQCLAAEN
HGVVDGPGVKVQEYHIMSSCYQRLLQIFHGLFAWSGFSQPEN QNLLYSALHVLSSRLKQGEHSQPLE
ELLSQSVHYLQNFHQSIPSFQCALYLIRLLMVILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSNIS
NDQLH ALLCIYLEHTESILKAIEEIAGVGVPELINSPKDASSSTFPTLTRHTFVVFFRVMMAELEKTVKKIE
PGTAADSQQIHEEKLLYWNMAVRDFSILINLIK VFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRK
HREDVLSLLETFQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLTLNNCREA FWLGN
LKNRDLQGEEIKSQNSQESTADESEDDMSSQASKSKATEDGEEDEVSAGEKEQDSDESYDDSD
FANCI
Homo sapiens
Spodoptera frugiperda
LEVO
MHHHHHHENLYFQGKPIPNPLLGLDSTMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKV
AGALLRAIFKGSPCSEEAGTLRRRKIYTCCI QLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFIS
AVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQY VIQLTSMFKD
VPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDEL
LDVVTVPSGELRHVEGTIIL HIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQD
QVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVH SWDHVTQGLVELGFILMDS
YGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLL
SNIVMYAPLV LQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDA
RKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHS HYNSVANETFCLEIMDSLRRCLSQQAD
VRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCC
IQH CLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNI
CAFLVMGVCEVLIEYNFSISSFSKNRFEDILS LFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVS
SLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQN LCDITR
VLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADV
SVTQRTAFQIRQFQRSLLNLLSSQ EEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENS
REDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFA IVNLRTAAPTVCLLV
LSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSC
VDTLLKDLCKMYTTL TALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTG
EKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKV
NLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK
singleParticle
particle
0.5
8
20.0
C4H11NO3
Tris/HCl pH 8.0
100.0
NaCl
sodium chloride
2.0
C4H10O2S2
DTT
ETHANE
95
277.55
FEI VITROBOT MARK IV
Complex components were mixed then sample was exchanged into cryoEM buffer via a desalting column
JEOL CRYO ARM 300
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
DIRECT ELECTRON DE-64 (8k x 8k)
COUNTING
46.2
1
Stochastic gradient descent implemented in RELION
C1
24.0
FSC 0.143 CUT-OFF
4404
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD