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    <admin>
        <current_status>
            <date>2024-05-22</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-04-07</deposition>
            <header_release>2020-07-29</header_release>
            <map_release>2020-07-29</map_release>
            <update>2024-05-22</update>
        </key_dates>
        <title>Rix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)</title>
        <authors_list>
            <author>Kater L</author>
            <author>Beckmann R</author>
        </authors_list>
        <keywords>pre-60S, Biogenesis, LSU, Large subunit, ribosome assembly, RIBOSOME</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Kater L</author>
                    <author order="2">Mitterer V</author>
                    <author order="3">Thoms M</author>
                    <author order="4">Cheng J</author>
                    <author order="5">Berninghausen O</author>
                    <author order="6">Beckmann R</author>
                    <author order="7">Hurt E</author>
                    <title>Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.</title>
                    <journal_abbreviation>Mol.Cell</journal_abbreviation>
                    <country>US</country>
                    <volume>79</volume>
                    <first_page>615</first_page>
                    <last_page>628.e5</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32668200</external_references>
                    <external_references type="DOI">doi:10.1016/j.molcel.2020.06.032</external_references>
                    <external_references type="ISSN">1097-2765</external_references>
                    <external_references type="CSD">2168</external_references>
                    <external_references type="ASTM">MOCEFL</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6yle</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Rix1-Rea1 pre-60S assembly particle - Rix1-subcomplex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Rix1-Rea1 pre-60S assembly particle - Rix1-subcomplex</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>Rix1-TAP Flag-Rea1 derived pre-60S assembly complex</details>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Pre-rRNA-processing protein IPI3</name>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.061836695</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MDEQVIFTTNTSGTIASVHSFEQINLRQCSTQSRNSCVQVGNKYLFIAQAQKALINVYNLSGSFKRESVEQRLPLPEILK
CLEVVENDGVQYDRIQGVNHNLPDFNLPYLLLGSTESGKLYIWELNSGILLNVKPMAHYQSITKIKSILNGKYIITSGND
SRVIIWQTVDLVSASNDDPKPLCILHDHTLPVTDFQVSSSQGKFLSCTDTKLFTVSQDATIRCYDLSLIGSKKKQKANEN
DVSIGKTPVLLATFTTPYSIKSIVLDPADRACYIGTAEGCFSLNLFYKLKGNAIVNLLQSAGVNTVQKGRVFSLVQRNSL
TGGENEDLDALYAMGQLVCENVLNSNVSCLEISMDGTLLLIGDTEGKVSIAEIYSKQIIRTIQTLTTSQDSVGEVTNLLT
NPYRLERGNLLFEGESKGKQPSNNNGHNFMKIPNLQRVIFDGKNKGHLHDIWYQIGEPEAETDPNLALPLNDFNAYLEQV
KTQESIFSHIGKVSSNVKVIDNKIDATSSLDSNAAKDEEITELKTNIEALTHAYKELRDMHEKLYEEHQQMLDKQ</string>
                    <external_references type="UNIPROTKB">P53877</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Pre-rRNA-processing protein RIX1</name>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.086839844</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSEEFIAVSTLARNLEIAKGNEFHTILATLRSPVYINEQLLKSELSFLVTKILKLIRSGNDFDLWKGCHTSVVTCAYNPL
VLSTHGGQLLAAIYSRLEQKTGFYSSVISSSHGKQLFNTLISSVAIIIDLMKNKPTLSREALVPKLKAIIPTLITLSQYE
PELVLPVLQRILKRNTTTFKPFTNKFRTVLINLIISDYASLGTKTQRLVCENFAYLHLLKIQVSDTSDDETQAHHKIYAD
SNWRTGLMSILSQFKPIIQLCGEILDFEQDNELYKLIKSLPVIDESNNKEEFLPSLKLDFNAPLTLWEIPQRLSLLADML
VAFISLPTPFPIRVPLGGINSLCEVLLGVSNKYLPLKKELRHDNELNGVINTILPQIQFQGIRLWEIMVSKYGKCGLSFF
EGILSSIELFIPLKKKSNNEIDFNVVGSLKFEFATVFRLVNMILSHLGHQLNIISVISQLIEVALFLSHDKTLIDSLFKN
RKSIMKQQTKTKQSKRSKSAEGAFSDIYTHPELFVCKNSMNWFNEINDFFITALNNWILPSTPHIQILKYSITQSLRLKE
RFGYIPESFVNLLRCEVLHPGSERVSILPIAISLLKNINDDMFELLCHPKVPVGMVYQLHKPLDLGEDGEVRDDINKKEV
ETNESSSNANTGLETLKALENLENVTIPEPKHEVPKVVDDTAIFKKRSVEEVIERESTSSHKKVKFVEETTVDNGEELIV
KKAVSQTKEEEKPMEDSEDEEQEEFEIPAIELSDDEEEEEEEE</string>
                    <external_references type="UNIPROTKB">P38883</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Pre-rRNA-processing protein IPI1</name>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.037928895</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTKSRKQKQKKQDFLRKKLKVGKPKEKARNATDTSFVSKTISIRNQHLDQNPHDLTKRLTLLKHHNINVRKETLTTFQKS
IPSIIKSRLMTPLLTQSIPLICDESQQVRQGLIDLVDEIGSHDAEILKLHCNIFVLYINMAMTHIVTQIQADSTKFLSHL
LKYCGDEVVRKSWVKLLNGVFGVLGWGQVGKNDSASIVQTKKRNAKYVTIHLNALYTLVEYGCQDERARSDGDTAETTED
SGTLRNPYLIPDYPQPFEHLKLFTRELKVQDATSSGVNATLLSLATQDIDTRKAVFIEQFLPIVRKKIEVIIKEGGECGK
SANKLKTLLAKIFD</string>
                    <external_references type="UNIPROTKB">A6ZSZ4</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil R3/3</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>CONTINUOUS</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">20</time>
                            <atmosphere>AIR</atmosphere>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 QUANTUM (4k x 4k)</film_or_detector_model>
                            <detector_mode>INTEGRATING</detector_mode>
                            <digitization_details>
                                <dimensions>
                                    <width units="pixel">3838</width>
                                    <height units="pixel">3710</height>
                                </dimensions>
                                <frames_per_image>1-48</frames_per_image>
                            </digitization_details>
                            <number_grids_imaged>1</number_grids_imaged>
                            <average_exposure_time units="s">10.0</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">75.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>273799</number_selected>
                </particle_selection>
                <startup_model type_of_model="EMDB MAP">
                    <emdb_id>EMD-3199</emdb_id>
                </startup_model>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <algorithm>FOURIER SPACE</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">3.3</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3.0.8</version>
                        </software>
                    </software_list>
                    <number_images_used>114398</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3.0.8</version>
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                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
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                        <software>
                            <name>RELION</name>
                            <version>3.0.8</version>
                        </software>
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                <final_three_d_classification>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3.0.8</version>
                        </software>
                    </software_list>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_10836.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
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            <col>200</col>
            <row>200</row>
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        <spacing>
            <x>200</x>
            <y>200</y>
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        <cell>
            <a units="Å">211.8</a>
            <b units="Å">211.8</b>
            <c units="Å">211.8</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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            <fast>X</fast>
            <medium>Y</medium>
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            <minimum>-0.037551817</minimum>
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            <y units="Å">1.059</y>
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                <level>0.025</level>
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        <label>::::EMDATABANK.org::::EMD-10836::::</label>
        <annotation_details>Rix1-subcomplex of the Rix1-Rea1 pre-60S particle</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>AB INITIO MODEL</refinement_protocol>
            </modelling>
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                    <col>200</col>
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                    <a units="Å">211.8</a>
                    <b units="Å">211.8</b>
                    <c units="Å">211.8</c>
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                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-10836::::</label>
                <annotation_details>Relion half map (unfiltered, unmasked)</annotation_details>
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                <file>emd_10836_half_map_1.map.gz</file>
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                    <space_group>1</space_group>
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                <dimensions>
                    <col>200</col>
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                    <b units="Å">211.8</b>
                    <c units="Å">211.8</c>
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                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
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                    <medium>Y</medium>
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                        <source>AUTHOR</source>
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                <label>::::EMDATABANK.org::::EMD-10836::::</label>
                <annotation_details>Relion half map (unfiltered, unmasked)</annotation_details>
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