2020-08-12 REL PDBe PDBe PDBe 2020-04-06 2020-05-20 2020-05-20 2020-08-12 Alexander von Humboldt Foundation SFB807 Germany Tobacco mosaic virus (TMV) structure determined by a hybrid STA-SPA workflow Sanchez RM Zhang Y Chen W Dietrich L Kudryashev M Sanchez RM Zhang Y Chen W Dietrich L Kudryashev M Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM. Nat Commun UK 11 3709 3709 2020 32709843 doi:10.1038/s41467-020-17466-0 2041-1723 Sanchez RM Zhang Y Chen W Dietrich L Kudryashev M Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM. Nat Commun UK 11 3709 3709 2020 32709843 doi:10.1038/s41467-020-17466-0 2041-1723 Sanchez RM Zhang Y Chen W Dietrich L Kudryashev M Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging - single particle cryoEM - workflow Biorxiv US 2020 doi:10.1101/2020.04.24.057299 EMD-10834 associated EM volume Tobacco mosaic virus Tobacco mosaic virus 0 Tobacco mosaic virus Capsid protein 180.0 VIRION STRAIN false false subtomogramAveraging helicalArray 33 7.4 Quantifoil R1.2/1.3 GOLD 400 GLOW DISCHARGE ETHANE
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 -0.0035 -0.0045 64000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 2.3 1 1.41 22.033 C1 5.24 FSC 0.143 CUT-OFF RELION 3.0 17998 4 20214 MAXIMUM LIKELIHOOD emd_10834.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 440.0 440.0 440.0 90.0 90.0 90.0 X Y Z -0.018232355 0.060033157 -0.0005931545 0.008035485 2.2 2.2 2.2 0.035 AUTHOR refine map emd_10834_msk_1.map emd_10834_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 440.0 440.0 440.0 90.0 90.0 90.0 X Y Z -0.038765598 0.07268425 -0.0005335857 0.008534766 2.2 2.2 2.2 AUTHOR half map 2 emd_10834_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 440.0 440.0 440.0 90.0 90.0 90.0 X Y Z -0.026771355 0.06862225 -0.0006741945 0.008456323 2.2 2.2 2.2 AUTHOR half map 1