2020-06-10 REL PDBe PDBe PDBe 2020-04-04 2020-04-29 2020-04-29 2020-06-10 French National Research Agency Chromcomp France French National Research Agency Chrom3D France French National Research Agency Chrome France French National Research Agency EPIVAR.Z France Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle (class S2) Boopathi R Danev R Khoshouei M Kale S Nahata S Ramos L Angelov D Dimitrov S Hamiche A Petosa C Bednar J Boopathi R Danev R Khoshouei M Kale S Nahata S Ramos L Angelov D Dimitrov S Hamiche A Petosa C Bednar J Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle reveals differential flexibility of the DNA ends. Nucleic Acids Res. UK 48 5735 5748 2020 32313946 doi:10.1093/nar/gkaa246 1362-4962 0389 NARHAD EMD-10825 associated EM volume CENP-A Nucleosome core particle with 145 base pairs of Widom 601 sequence CENP-A Nucleosome core particle with 145 base pairs of Widom 601 sequence 0 1 2 3 4 5 6 CENP-A 1 1 Homo sapiens Escherichia coli Histones H2A, H2B, H4 1 2 3 4 Xenopus laevis Escherichia coli Widom 601 DNA 1 5 6 synthetic construct Escherichia coli singleParticle particle 7.4 Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY GLOW DISCHARGE OTHER ETHANE-PROPANE 95 277 FEI VITROBOT MARK IV
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.5 0.3 0.5 0.3 47000.0 47000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 2-38 2 2608 7.6 50.0 1 227552 Gctf 1.06 https://www.mrc-lmb.cam.ac.uk/kzhang/
Low-pass filtered EM map of the canonical H3 nucleosome core particle
 1 C1 FOURIER SPACE 5.1 FSC 0.143 CUT-OFF RELION 2.03 30915 PROJECTION MATCHING RELION 2.03 PROJECTION MATCHING RELION 2.03 6 RELION 2.03 emd_10825.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 140 140 140 0 0 0 140 140 140 148.4 148.4 148.4 90.0 90.0 90.0 X Y Z -0.037146475 0.07996334 0.0010390878 0.006872173 1.06 1.06 1.06 0.0184 AUTHOR Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle (class S2)