REL
PDBe
PDBe
PDBe
2004-05-17
2004-05-25
2005-05-25
2012-10-17
The PM2 virion has a novel organization with an internal membrane and pentameric receptor binding spikes.
Huiskonen JT
Kivela HM
Bamford DH
Butcher SJ
Huiskonen JT
Kivela HM
Bamford DH
Butcher SJ
The PM2 virion has a novel organization with an internal membrane and pentameric receptor binding spikes.
NAT.STRUCT.MOL.BIOL.
11
850
856
2004
15286721
doi:10.1038/nsmb807
PM2 virion
PM2 virion
Purified virion (1 mg/ml) was purified, pelleted and
resuspended just prior to application to the grid.
1
33.8
Reported mass of the whole virion in the literature is
47 MDa, 72 percent of which is protein.
Pseudoalteromonas phage PM2
includes mass of DNA, lipid and protein
Pseudoalteromonas phage PM2
Pseudoalteromonas sp. ER72M2
BACTERIA(EUBACTERIA)
47
P2
597
21
virion (spike-spike distance)
765
21
VIRION
STRAIN
true
false
PM2
singleParticle
particle
4
7.2
CaCl2, 5mM Tris-HCl, 10-20 mM NaCl, 25-125 mM
400 mesh copper grid, R2/2 quantifoil holey
ETHANE
90
HOMEMADE PLUNGER
Vitrification instrument: EMBL design
A small vial of ethane is placed inside a larger
liquid nitrogen reservoir. The grid holding 3
microliters of the sample is held in place at the
bottom of a plunger by the means of fine tweezers.
When the liquid ethane is ready, a piece of
filter paper is then pressed against
the sample to blot off excess buffer,
sufficient to leave a thin layer on the grid. The
filter paper is removed, and the plunger is
allowed to drop into the liquid ethane. Once
the grid enters the liquid ethane, the sample is
rapidly frozen, and the grid is transferred under liquid
nitrogen to a storage box immersed in liquid
nitrogen for later use in the microscope.
FEI TECNAI F20
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
200
2
0.6
2.9
50000.0
GATAN LIQUID NITROGEN
90
94
93
objective lens astigmatism was corrected at
A box anticontaminator was fitted with a minimum
achievable temperature of 88 K
KODAK SO-163 FILM
ZEISS SCAI
7
111
10
12.
Side entry liquid nitrogen-cooled cryo specimen holder
The particles were first automatically selected
using ETHAN, and then boxed out manually in EMAN.
each particle, wiener factor 0.1
I
OTHER
8.4
FSC 0.5 CUT-OFF
EMBL, P3DR
13887