2024-05-22 REL PDBe PDBe PDBe 2020-04-02 2020-09-16 2020-09-16 2024-05-22 Structure of the TFIIIC subcomplex tauA Vorlaender MK Muller CW TFIIIC, tauA, Transcription initiation, Pol III, TFIIIB, Transcription factor, TRANSCRIPTION Vorlander MK Jungblut A Karius K Baudin F Grotsch H Kosinski J Muller CW Structure of the TFIIIC subcomplex tau A provides insights into RNA polymerase III pre-initiation complex formation. Nat Commun UK 11 4905 4905 2020 32999288 doi:10.1038/s41467-020-18707-y 2041-1723 6yj6 FULLOVERLAP TFIIIC subcomplex tauA TFIIIC subcomplex tauA 0 1 2 3
Generated by co-expression of subunits in insect cells
Saccharomyces cerevisiae 0.245 Transcription factor tau 131 kDa subunit Saccharomyces cerevisiae 0.120746969 1 Trichoplusia ni LEVO MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLIQDEEYDDEDVPHDLQLSEDEYNSERDSSLLAEFSDY GEISEDDEEDFMNAIREASNFKVKKKKKNDKGKSYGRQRKERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDAR NFAAYETLGDIYQLQGRLNDCCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYCFSRVISLNPMEWESIYRRSMLY KKTGQLARALDGFQRLYMYNPYDANILRELAILYVDYDRIEDSIELYMKVFNANVERREAILAALENALDSSDEESAAEG EDADEKEPLEQDEDRQMFPDINWKKIDAKYKCIPFDWSSLNILAELFLKLAVSEVDGIKTIKKCARWIQRRESQTFWDHV PDDSEFDNRRFKNSTFDSLLAAEKEKSYNIPIDIRVRLGLLRLNTDNLVEALNHFQCLYDETFSDVADLYFEAATALTRA EKYKEAIDFFTPLLSLEEWRTTDVFKPLARCYKEIESYETAKEFYELAIKSEPDDLDIRVSLAEVYYRLNDPETFKHMLV DVVEMRKHQVDETLHRISNEKSSNDTSDISSKPLLEDSKFRTFRKKKRTPYDAERERIERERRITAKVVDKYEKMKKFEL NSGLNEAKQASIWINTVSELVDIFSSVKNFFMKSRSRKFVGILRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVT LTSATELRGLSYEQWFELFMELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKFVKLAIVLQMDDEEELAENLR GLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFLRQLKAFDSCRYNTEVNGQASITNKEVYNPNKKSSPYLYYI YAVLLYSSRGFLSALQYLTRLEEDIPDDPMVNLLMGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQE ADYNLGRAFHLIGLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLMEKYLSIRSGG P33339 Transcription factor tau 95 kDa subunit,Transcription factor tau 95 kDa subunit Saccharomyces cerevisiae 0.077363664 1 Trichoplusia ni LEVO MHHHHHHENLYFQSMPVEEPLATLSSIPDSSADQAPPLIADEFTLDLPRIPSLELPLNVSTKHSSIQKAIKMCGGIEKVK EAFKEHGPIESQHGLQLYLNDDTDSDGSKSYFNEHPVIGKRVPFRDESVILKVTMPKGTLSKNNNSVKDSIKSLKDSNKL RVTPVSIVDNTIKFREMSDFQIKLDNVPSAREFKSSFGSLEWNNFKSFVNSVPDNDSQPQENIGNLILDRSVKIPSTDFQ LPPPPKLSMVGFPLLYKYKANPFAKKKKNGVTEVKGTYIKNYQLFVHDLSDKTVIPSQAHEQVLYDFEVAKKTKVYPGTK SDSKFYESLEECLKILRELFARRPIWVKRHLDGIVPKKIHHTMKIALALISYRFTMGPWRNTYIKFGIDPRSSVEYAQYQ TEYFKIERKLLSSPIVKKNVPKPPPLVFESDTPGGIDSRFKFDGKRIPWYLMLQIDLLIGEPNIAEVFHNVEYLDKANEL TGWFKELDLVKIRRIVKYELGCMVQGNYEYNKYKLKYFKTMLFVKESMVPENKNSEEGMGVNTNKDADGDINMDAGSQMS SNAIEEDKGIAAGDDFDDNGAITEEPDDAALENEEMDTDQNLKVPASIDDDVDDVDADEEEQESFDVKTASFQDIINKIA KLDPKTAETMKSELKGFVDEVDL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) P32367 Transcription factor tau 55 kDa subunit Saccharomyces cerevisiae 0.049198898 1 Trichoplusia ni LEVO MVVNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQAKELAHYLLSLDNQPEAAFASPFYRCLETVQPIAKLL EIPVYLERGIGEWYRPDRKPVIPVPAGYEILSKFFPGVISQEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPN VECILLVTHAASKIALGMSLLGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDTIDETDDQTSFTYIPFSDRKWVLTM NGNTEFLSSGEEMNWNFDCVAEAGSDADIKKRQMTKKTSSPIPEADDQTEVETVYISVDIPSGNYKERTEIAKSAILQYS GLETDAPLFRIGNRLYEGSWERLVGTELAFPNAAHVHKKTAGLLSPTEENETTNAGQSKGSSTANDPNIQIQEEDVGLPD STNTSRDHTGDKEEVQSEKIYRIKERIVLSNVRPM Q12415 singleParticle particle 1.3 7.5
20 mM HEPES, 5 mM DTT, 75 mM NaCl
 Quantifoil R2/1 COPPER 200 GLOW DISCHARGE
Pelco Easygglow instrument
 ETHANE 100 298 FEI VITROBOT MARK IV
Blot force 2, Blot time 4.
4 mM CHAPSO added prior to freezing
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 -0.5 -2.0 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 1-36 1 5824 48.73 1.35
collected 8 frames per hole using beam shift
 1 702583
BoxNet2_20180918 was used for particle picking
 obtained with Relion C1 3.1 FSC 0.143 CUT-OFF RELION 3.0 254700 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION 3.0 RELION 3.0 emd_10817.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 312.3 312.3 312.3 90.0 90.0 90.0 X Y Z -0.16542615 0.283146 -0.00016718963 0.0053780037 1.041 1.041 1.041 0.05 AUTHOR AB INITIO MODEL REAL emd_10817_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 312.3 312.3 312.3 90.0 90.0 90.0 X Y Z -0.034096267 0.06606487 -0.000084512496 0.0025515603 1.041 1.041 1.041 AUTHOR emd_10817_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 312.3 312.3 312.3 90.0 90.0 90.0 X Y Z -0.02773345 0.06609534 -0.0000852127 0.002557591 1.041 1.041 1.041 AUTHOR