REL PDBe PDBe PDBe 2004-05-13 2004-05-14 2004-07-14 2011-05-26 Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy. Ludtke SJ Chen D Song J Chuang DT Chiu W Ludtke SJ Chen DH Song JL Chuang DT Chiu W Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy. STRUCTURE 12 1129 1136 2004 15242589 doi:10.1016/j.str.2004.05.006 native naked GroEL native naked GroEL homo 14-mer 1 0.8 GroEL Escherichia coli 0.057 14 14mer true ESts CG-712 pGroESL singleParticle particle 1.0 ETHANE HOMEMADE PLUNGER
Vitrification instrument: Manual gravity plunger
 JEOL 2010F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 1.0 0.794 2.56 50000.0 GATAN LIQUID NITROGEN KODAK SO-163 FILM ZEISS SCAI 7 42 18
Data was median filtered to 10.5 microns/pixel after scanning.
8. Side entry
Note that class averages are EMAN-style reference-based, not MSA.
per-particle phase flipping pre-processing, amplitude correction during class averaging
 D7 6.0 FSC 0.5 CUT-OFF EMAN 39085 295 emd_1081.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 -64 -64 -64 128 128 128 264.16 264.16 264.16 90 90 90 X Y Z -1.19262 1.78203 -0.000000000096827 0.148809 2.08 2.08 2.08 0.209 This is the density map for the final published ~6 A resolution GroEL structure.
::::EMDATABANK.org::::EMD-1081::::
 1OEL RIGID BODY FIT foldhunter, EMAN
Protocol: Rigid Body. rigid body fitting of 1 monomer from 1OEL initially, followed by rigid body refinement of each domain, apical, intermediate and equatorial.
correlation coefficient REAL