REL PDBe PDBe PDBe 2004-05-06 2004-05-12 2004-05-12 2011-05-26 A 11.5 A single particle reconstruction of GroEL using EMAN. Ludtke SJ Jakana J Song J Chuang DT Chiu W Ludtke SJ Jakana J Song JL Chuang DT Chiu W A 11.5 A single particle reconstruction of GroEL using EMAN. J.MOL.BIOL. 314 253 262 2001 11718559 doi:10.1006/jmbi.2001.5133 native naked GroEL native naked GroEL homo 14-mer 1 0.8 GroEL Escherichia coli 0.057 14 14-mer true ESts CG-712 pGroESL singleParticle particle 1.0 ETHANE HOMEMADE PLUNGER
Vitrification instrument: Manual, gravity, plunger
 JEOL 4000EX FLOOD BEAM BRIGHT FIELD LAB6 400 4.1 1.0 2.4 50000.0 GATAN LIQUID NITROGEN KODAK SO-163 FILM ZEISS SCAI 7 5 30
Data was median filtered to 14 microns/pixel after scanning.
8. Side entry
Note that class averages are EMAN-style reference-based, not MSA.
Per particle phase flipping, amplitude correction during averaging
 D7 11.5 FSC 0.5 CUT-OFF EMAN 4916 334 emd_1080.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 -50 -50 -50 100 100 100 267.3 267.3 267.3 90 90 90 X Y Z -1.34116 2.36503 0.0813288 0.324536 2.7 2.7 2.7 0.852 This is the volumetric data for our published 11.5 A resolution reconstruction of GroEL. Note that newer EMAN releases can refine the same data to ~9 A resolution.
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 1OEL RIGID BODY FIT foldhunter EMAN
Protocol: Rigid Body
correlation coefficient REAL