2020-10-14 REL PDBe PDBe PDBe 2020-03-25 2020-04-01 2020-09-23 2020-10-14 Negative stain map of S. cerevisiae Brf1 and TBP bound to the TFIIIC-subcomplex tauA Vorlaender MK Muller CW Vorlander MK Jungblut A Karius K Baudin F Grotsch H Kosinski J Muller CW Structure of the TFIIIC subcomplex tau A provides insights into RNA polymerase III pre-initiation complex formation. Nat Commun UK 11 4905 4905 2020 32999288 doi:10.1038/s41467-020-18707-y 2041-1723 EMD-10795 associated EM volume Complex between Brf1, TBP, and the tauA subcomplex of TFIIIC Complex between Brf1, TBP, and the tauA subcomplex of TFIIIC 0 1 2 3 4 5
Crosslinked with Glutaraldehyde in absence of DNA
0.3 Transcription factor tau 131 kDa subunit, Transcription factor tau 95 kDa subunit and Transcription factor tau 55 kDa subunit 1 1 2 3 Saccharomyces cerevisiae Trichoplusia ni Trichoplusia ni Trichoplusia ni Transcription factor IIIB 70 kDa subunit and TATA-box-binding protein 1 4 5 Saccharomyces cerevisiae Escherichia coli Escherichia coli Transcription factor tau 131 kDa subunit Saccharomyces cerevisiae Trichoplusia ni LEVO MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLIQDEEYDDEDVPHDLQL SEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFKVKKKKKNDKGKSYGRQRK ERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDARNFAAYETLGDIYQLQGRLND CCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYCFSRVISLNPMEWESIYRRSMLY KKTGQLARALDGFQRLYMYNPYDANILRELAILYVDYDRIEDSIELYMKVFNANVERREA ILAALENALDSSDEESAAEGEDADEKEPLEQDEDRQMFPDINWKKIDAKYKCIPFDWSSL NILAELFLKLAVSEVDGIKTIKKCARWIQRRESQTFWDHVPDDSEFDNRRFKNSTFDSLL AAEKEKSYNIPIDIRVRLGLLRLNTDNLVEALNHFQCLYDETFSDVADLYFEAATALTRA EKYKEAIDFFTPLLSLEEWRTTDVFKPLARCYKEIESYETAKEFYELAIKSEPDDLDIRV SLAEVYYRLNDPETFKHMLVDVVEMRKHQVDETLHRISNEKSSNDTSDISSKPLLEDSKF RTFRKKKRTPYDAERERIERERRITAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSEL VDIFSSVKNFFMKSRSRKFVGILRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVT LTSATELRGLSYEQWFELFMELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKF VKLAIVLQMDDEEELAENLRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFL RQLKAFDSCRYNTEVNGQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTR LEEDIPDDPMVNLLMGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQE ADYNLGRAFHLIGLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLME KYLSIRSGG Transcription factor tau 95 kDa subunit Saccharomyces cerevisiae Trichoplusia ni LEVO MHHHHHHENLYFQSMPVEEPLATLSSIPDSSADQAPPLIADEFTLDLPRIPSLELPLNVS TKHSSIQKAIKMCGGIEKVKEAFKEHGPIESQHGLQLYLNDDTDSDGSKSYFNEHPVIGK RVPFRDESVILKVTMPKGTLSKNNNSVKDSIKSLKDSNKLRVTPVSIVDNTIKFREMSDF QIKLDNVPSAREFKSSFGSLEWNNFKSFVNSVPDNDSQPQENIGNLILDRSVKIPSTDFQ LPPPPKLSMVGFPLLYKYKANPFAKKKKNGVTEVKGTYIKNYQLFVHDLSDKTVIPSQAH EQVLYDFEVAKKTKVYPGTKSDSKFYESLEECLKILRELFARRPIWVKRHLDGIVPKKIH HTMKIALALISYRFTMGPWRNTYIKFGIDPRSSVEYAQYQTEYFKIERKLLSSPIVKKNV PKPPPLVFESDTPGGIDSRFKFDGKRIPWYLMLQIDLLIGEPNIAEVFHNVEYLDKANEL TGWFKELDLVKIRRIVKYELGCMVQGNYEYNKYKLKYFKTMLFVKESMVPENKNSEEGMG VNTNKDADGDINMDAGSQMSSNAIEEDKGIAAGDDFDDNGAITEEPDDAALENEEMDTDQ NLKVPASIDDDVDDVDADEEEQESFDVKTASFQDIINKIAKLDPKTAETMKSELKGFVDE VDL Transcription factor tau 55 kDa subunit Saccharomyces cerevisiae Trichoplusia ni LEVO MVVNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQAKELAHYLLSLDNQPEA AFASPFYRCLETVQPIAKLLEIPVYLERGIGEWYRPDRKPVIPVPAGYEILSKFFPGVIS QEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPNVECILLVTHAASKIALGMSL LGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDTIDETDDQTSFTYIPFSDRKWVLTM NGNTEFLSSGEEMNWNFDCVAEAGSDADIKKRQMTKKTSSPIPEADDQTEVETVYISVDI PSGNYKERTEIAKSAILQYSGLETDAPLFRIGNRLYEGSWERLVGTELAFPNAAHVHKKT AGLLSPTEENETTNAGQSKGSSTANDPNIQIQEEDVGLPDSTNTSRDHTGDKEEVQSEKI YRIKERIVLSNVRPM Transcription factor IIIB 70 kDa subunit Saccharomyces cerevisiae Escherichia coli LEVO MSALESREATLNNARRKLRAVSYALHIPEYITDAAFQWYKLALANNFVQGR RSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSVYSIGATFLKMVKKLHITELPLADPSL FIQHFAEKLDLADKKIKVVKDAVKLAQRMSKDWMFEGRRPAGIAGACILLACRMNNLRRT HTEIVAVSHVAEETLQQRLNEFKNTKAAKLSVQKFRENDVEDGEARPPSFVKNRKKERKI KDSLDKEEMFQTSEEALNKNPILTQVLGEQELSSKEVLFYLKQFSERRARVVERIKATNG IDGENIYHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENES GHFQDAIDGYSLETDPYCPRNLHLLPTTDTYLSKVSDDPDNLEDVDDEELNAHLLNEEAS KLKERIWIGLNADFLLEQESKRLKQEADIATGNTSVKKKRTRRRNNTRSDEPTKTVDAAA AIGLMSDLQDKSGLHAALKAAEESGDFTTADSVKNMLQKASFSKKINYDAIDGLFRLEHHHHHH TATA-box-binding protein Saccharomyces cerevisiae Escherichia coli LEVO SGIVPTLQNIVATVTLGCRLDLKTVALHARNAEYNPKRFAAVIMRIREPKTTALIFASGK MVVTGAKSEDDSKLASRKYARIIQKIGFAAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHG TFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYQAFEAIYPVLSEFRKM singleParticle particle 7.5 NEGATIVE Uranyl Acetate
Crosslinked sample
 FEI TECNAI SPIRIT FLOOD BEAM BRIGHT FIELD LAB6 120 GATAN ULTRASCAN 4000 (4k x 4k) 20.0 1 Ab initio model generated in CryoSPARC C1 32.36 FSC 0.143 CUT-OFF RELION 3.0 10731 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION 3.0 emd_10795.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 458.40002 458.40002 458.40002 90.0 90.0 90.0 X Y Z -0.063415006 0.20393701 -0.00061397516 0.009914577 2.292 2.292 2.292 0.05 AUTHOR Refined map RIGID BODY FIT Envelope score emd_10795_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 458.40002 458.40002 458.40002 90.0 90.0 90.0 X Y Z -0.07194382 0.20619136 -0.0005446783 0.010372211 2.292 2.292 2.292 AUTHOR Half map 2 emd_10795_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 458.40002 458.40002 458.40002 90.0 90.0 90.0 X Y Z -0.076365225 0.21365602 -0.00058507686 0.010373087 2.292 2.292 2.292 AUTHOR Half map 1