Electron crystallography reveals the structure of metarhodopsin I.

REL PDBe PDBe PDBe 2004-05-12 2004-05-12 2004-09-07 2013-09-25 Electron crystallography reveals the structure of metarhodopsin I. Ruprecht JJ Mielke T Vogel R Villa C Schertler GFX Ruprecht JJ Mielke T Vogel R Villa C Schertler GF Electron crystallography reveals the structure of metarhodopsin I. EMBO J. 23 3609 3620 2004 15329674 doi:10.1038/sj.emboj.7600374 Bovine Metarhodopsin I Bovine Metarhodopsin I

Illuminated to form a photostationary state highly enriched in metarhodopsin I

monomer 1 0.039 0.039 Rhodopsin Bos taurus Bovine Retina Retinal rod cell Membrane Disk membrane 0.039 0.039

Illuminated to form photostationary state

1 Monomer false GO:0009586 IPR000732 electronCrystallography twoDArray 0.5 7.0

20mM HEPES pH 7, 100 mM NaCl, 10 mM MgCl2, 3 mM NaN3, 4 mM DTT, 4 mM mercaptoethanol, 2.5 % (v/v) isopropanol

300 mesh molybdenum grid

ETHANE 75 77 GATAN CRYOPLUNGE 3

Vitrification instrument: Gatan cryoplunge. Vitrification carried out in temperature and humidity-controlled chamber

Vitrified after illumination of specimen Blot for 20 sec before plunging

11 day dialysis at 18 deg C

FEI TECNAI F30 SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.3 0.27 1.41 60000.0 59000.0 GATAN LIQUID NITROGEN 77 77 77 corrected at 230,000 times magnification KODAK SO-163 FILM ZEISS SCAI 7 87 15 Side entry liquid-nitrogen cooled Gatan holder 0 60 0 60 OTHER 5.5 OTHER MRC 58.8 83.7 200.0 90 90 90 P 2 21 21

Each image

emd_1079.map.gz 18 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 231 181 119 -20 -30 -59 190 120 120 58.800003 83.69994 200.0004 90.0 90.0 90.0 Y X Z -131.953247070000003 250.0 -0.29949433 28.60485268 0.49000004 0.440526 1.66667 25.0 AUTHOR Density map of a rhodopsin photostationary state, highly enriched in metarhodopsin I

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1GZM B RIGID BODY FIT O

PDBEntryID_givenInChain. Protocol: Rigid Body

REAL