REL PDBe PDBe PDBe 2004-05-07 2004-05-07 2004-05-07 2011-05-26 Lumbricus terrestris hemoglobin--the architecture of linker chains and structural variation of the central toroid. Mouche F Boisset N Penczek PA Mouche F Boisset N Penczek PA Lumbricus terrestris hemoglobin--the architecture of linker chains and structural variation of the central toroid. J.STRUCT.BIOL. 133 176 192 2001 11472089 doi:10.1006/jsbi.2001.4362 Lumbricus terrestris hemoglobin Lumbricus terrestris hemoglobin 12 x 12 mer 1 3.6 Lumbricus terrestris hemoglobin Lumbricus terrestris earthworm blood 3.6 144 12 x 12 mer false singleParticle particle 0.5 7.2
50 mM Tris-HCl, 50 mM MgCl2, 10 mM CaCl2
 NEGATIVE
NO STAIN Cryo-electron microscopy
400 mesh gold grid
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: home made
Single side blotting and rapid plunging JEOL 2010F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 1.0 1.3 3.2 60000.0 66489.0 GATAN LIQUID NITROGEN 86 86 86 0 1999-10-17 KODAK SO-163 FILM OPTRONICS 2.5 16
Rotating drum microdensitometer
1.0 16. Side entry liquid nitrogen-cooled cryo specimen holder 0 0
Images recorded at 200 kV with a magnification of x60000 on a Jeol JEM2010F. Condensor aperture 150 um. Objective aperture 60 um. Defocus within a range of 1.3 to 3.2 um.
Wiener filtering on volumes
 D6 OTHER 14.9 FSC 0.5 CUT-OFF SPIDER
SIRT on single 2D-projections
7770
SPIDER: theta 90 degrees, phi 60 degrees
 emd_1078.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 81 81 81 -40 -40 -40 81 81 81 304.56 304.56 304.56 90 90 90 X Y Z 0.0 1.0 0.341941 0.099831 3.76 3.76 3.76 0.559 Whole structure of Lumbricus terrestris hemoglobin
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 emd_1078_fsc.xml