55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST)

2024-10-16 REL PDBe PDBe PDBe 2020-03-21 2020-07-15 2020-07-15 2024-10-16 European Molecular Biology Organization (EMBO) 1196-2014 Switzerland Swiss National Science Foundation 310030B_163478 Switzerland Swiss National Science Foundation 138262 Switzerland 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Kummer E Ban N Mitochondria, 55S ribosome, mtEFG1, Elongation, TRANSLATION Kummer E Ban N Structural insights into mammalian mitochondrial translation elongation catalyzed by mtEFG1. Embo J. UK 39 e104820 e104820 2020 32602580 doi:10.15252/embj.2020104820 1460-2075 0897 EMJODG 6ydw FULLOVERLAP 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 2.8 55S S. scrofa mitochondrial ribosome 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 Sus scrofa tRNAMet and mRNA 1 46 47 Homo sapiens Elongation factor G, mitochondrial 1 75 Homo sapiens Uncharacterized protein Sus scrofa 0.04560457

residues 187-194 built as poly-Ala

1 LEVO MAAPCVFCGAAVSYRLFLGCRVSFARGQVLWKAAAPELQAGARCQILRLRHTAVVTTKKNVAASRRETYTVDFIKKQIEE FNIGKRHLANMMGEDPETFTQEDIDRAIAYLFPSGLFEKRARPIMKHPEEIFPKQRAIQWGEDGRPFHFLFYTGKQSYYS LMHDTYGKLLDVEKHHNQLRAKDLLAEKTKILKDPIGSRWLIKEELEEMLVEKLSDQDYAQFIRLLERLSALPCGATEED FVNRFRRSIPIQSKKQLIEPLQYDEQGMAFSRGEGKRKTAKAEVVVYGQGSGRIDVNGVDYLLYFPVTQDREQLMFPLHF LDRLGKHDMTCAVSGGGRSAQAGAVRLAMARALCSFVTEDEVEWMRQAGLLTADPRVRERKKPGQEGARRKFTWKKR I3LU08 Mitochondrial ribosomal protein L19 Sus scrofa 0.03344153899999999 1 LEVO MAASITRGSWAAVGLGQSFPPTRALLPAPASVICRTLAGSGRQQVTGSSEPGAFQPPPKPVIVDKRRPQRPETRFLSPEF IPPRGRTNPLKFQIERKDMLERRKILHIPEFYVGSILRVTTADPYASAKTSQFLGICIQRSGSGLGATFILRNTIEGQGV EICFELYNPRIQEIQVVKLEKRLDNSLLYLRDALPEYSTFDVNMKPITQEFNQEVPVNQLKVKMKPKPWSKRWERPKFNI KGIRFDLCLTEEQMREAQKWSQPWLEFDMMREYDTSKIEAAIWDEIEASKNS I3LNJ0 Mitochondrial ribosomal protein L20 Sus scrofa 0.017554793 1 LEVO MVFLTVPLWLRSRVTDRYWRVQEVLKHARHFRGRKNRCYRLAVRAVTRAFVKCTRARRLKKRSLRTLWINRITAASQEHG LKYPAFIINLIKCQVELNRKVLADLAIYEPKTFKSLAALAKRRREEGFAAALGDGKEPDGIFSRVVQHR A0A286ZU56 Mitochondrial ribosomal protein L21 Sus scrofa 0.023154861 1 LEVO MAAAVAASVLQGTFGRLVSAYSRSVLRTSRPGTAPPWSAPRRLSSQHTSSLRGCVPKTSLGSPPWPDIVLPDPVEETRHH AEVVGQVNELIAAGRYGRLFAVVHFAGHQWKVTAEDLILIENELDVACGERIRLEKVLLVGADNFTLLGKPLLGKELVRV EATVIEKTESWPKINMKFQKRKNYQRKRITVNPQTVLRINSIEIAPCLC F1RY70 Uncharacterized protein Sus scrofa 0.024313356999999997 1 LEVO MAAAVLERLGGLWMQNLRGKLALRLLSSHSILPQSHIHTSASVEISRKWEKKNKIVYPPQLPGEPRRPAEIYHCRRQIKY SKDKMWYLAKLIRGMSIDQALAQLEFSDKKGAQIIKEVLLEAQDMAVRDHNVEFRSNLYVAESTSGRGQYLKRIRYHGRG RFGIMEKVFCHYFVKLVEGPPPPREAPKTAVAHAREYIQELRNRTITHAL F1RQC9 Uncharacterized protein Sus scrofa 0.017643205000000002

residues 115-133 built as poly-Ala

1 LEVO MARNVLYPLYQLGNPQLRVFRTNFFIQLVRPGTAQPEDTVQFRIPMEMTRVDLRNYLERIYNVPVAAVRTRVQYGSNRRR DHRNIRIKKPDYKVAYVQLALGQTFTFPDLFPERKGASVDVDVRDQVLEDQRQKHSPDPRRGGVPGWFGL A0A286ZZC6 Uncharacterized protein Sus scrofa 0.024893528999999998 1 LEVO MRLSALLALASKVTLPPNYRYGMSRPGSLSDKRKNPPGTRRRRVPVEPISDEDWHLFCGDKVEILEGKDAGKQGKVVQVI RQRNWVVVEGLNTHYRYVGKTVDYRGTMIPSEAPLLHNQVKLVDPTDRKPTDVEWRFTEAGERVRVSTRSGRIIPKPEFP RADGIVPETWIDGPKDTSVEDALEKTYVPRLKTLEEEVMEAMGIQETRRHKKVYWY F1RHJ1 Mitochondrial ribosomal protein L37 Sus scrofa 0.047917987999999995 1 LEVO MALASGPARRALARLGRLGLGGCGAPRRGAYEWGVRSTRKPEPPPLDRVYEIPGLEPITYAGKMHFMPGLARPVFPPWDP GWTHPKFRRLPPLHEHPLYKDQACYVFHQRCRLLEGVKQALWLTKTQLIEGLPEKVLRLADDPRNHIENQDERVLNAISH ARLWHSTEDIPKRETYCPVIVDSLIQLCKSQILKHPSLARRICAQNNTLSATWNRESILLQVHGSSGARLNAKDPLPPVA SQEEVEATKNHVLETFYPISPTMGLQECNVYDVNDDTGFQEGYPYPCPHTLYFLESANLRPRRFQPDQLRAKMILFAFGS ALAQARLLYGNDSKVLEQPVVVQSVGTDGRLFQFLVLQLNTTDLASDEGVKNLAWVDSDQLLYQHFWCLPVIKKKVVVEP VGPIGFQPETFRKFLALYLHGAV A0A287BIV6 Mitochondrial ribosomal protein L38 Sus scrofa 0.044656387 1 LEVO MAAPWWRAALCASRRWRGFSTSAALSRRAAPLGPMPNEDIDVSDLERLKKYRSFDRYRRRAEQEARKPHWWRTYREHFGE ESGPKDRVDIGLPPPKVSRTQQLLERKQALRELRANVEEERAARLQTARIPLEAVRAEWERTCGPYHKQRLAEYCGLYRD LFHGATFVPRVPLHVAYAVGEDDLMPVYHGNEVTPTEAAQAPEVTYEADEGSLWTLLLTNLDGHLLEPDAEYVHWLVTNI PGNRVTEGQETCPYLPPFPARGSGFHRFAFLLFKQDKRIDFSGDTRPSPCYQLAQRTFHTFDFYKKHQDAMTPAGLAFFQ CRWDDSVTRVFHQLLDMREPVFEFVRPPPYHPKQKRFPHRQPLRYLDRYRDSHEPTYGIY F1RW03 Mitochondrial ribosomal protein L39 Sus scrofa 0.038523421999999995 1 LEVO MAMGAWGLRLWRAVPRGEAGWRLIATSPASQLSPTELIEMQNDLFNKEKNRQLSLTPRTEKIEVKHVGKTDPGTVFVMNK NISTPYSCAMHLSEWYCRKSILALVDGQPWDMYKPLTKSCEIKFLTFKDDDPGEVNKAYWRSCAMMMGCVIERAFKDEYV VSLVRAPEVPVIAGAFCYDVVLDKRLDEWMPTKENLHSFTKDARALIYKDLPFETLEVEAKVALEIFQHNKYKLDFIEEK ASQNPERIVKLHRFGDFIDVSEGPLIPRTSICFQYEVSAVHNLQTQSSLVRRFQGLSLPVHLRAHFTIWNKLLERSRKMV TEDKTKPTEESAST F1SHP6 Uncharacterized protein Sus scrofa 0.024075658 1 LEVO MAAAALGAASRTLRPASRLVGAWPTQTRDAHERGSLFSFWGLVPMRAEPLRKKKKVDPKKDQAAKDRLKKRIRRLEKASQ ELIPIEDFITPVKFLNKERQRPPVELPFEESERRALLLKRWSLYKQREHEMERSAIRSLLEAQEEALQELRLSSPELHAE ATKRDPSLFPFERQGPDYTPPISDYQPPEGRYQDITKVYTQVEFKK A0A286ZP98 Mitochondrial ribosomal protein L41 Sus scrofa 0.015073404 1 LEVO MGLLSGAARALVRGADRMSKWTSKRGPRTFCKGRGAKGTGFHGRDGKFVQIKEMIPELVVPELAGFKLKPYVNYRAPEGT DTPLTAKQLFLETAAPAIEKDFKAGTFDPEHLEKYGFEPTQEGKLFQLYPKNFPR A0A287AWS0 Uncharacterized protein Sus scrofa 0.016680178

residues 77-100 built as poly-Ala

1 LEVO MALAAVKWVISSRTILKHLFPIQNSASYCVCHKSTYSSLPDDYNCKVELALTSDGRTIVCYHPSVDIPYEHTKPIPRPDP VHNIEETHDLVLKTRLEEKGEHLEQGPMIEQLSKMFFTTKHRWYPRGQYHRRRRKPNPPKDR A0A287BQA1 Mitochondrial ribosomal protein L43 Sus scrofa 0.017728109 1 LEVO MTARGTASRFLTSVLHNGLGRYVQQLQRLSFSLSRDAPSSRGAREFVEREVTDFARRNPGVVIYVNPRPCCVPRVVAEYL NGAVREESIHCKSVEEIAALVQKLADQSGLDVIRIRKPFHTDSPSIQGQWHPFTNKPTTLGGLRPREVQDPAPAQVQAQ F1S8U4 Uncharacterized protein Sus scrofa 0.037527195 1 LEVO MASGLVRLLQWGPRRLLAPAAPTLAPPVRGAKKGFRAAYRFQKELERWRLLRCPPPPVRRSEKPNWDYHAEIQAFGHRLQ ETFSLDLLKTAFVNSCYIKSEEAKRQKLGIDKEAALLNLKDNQELSEQGISFSQTCLTQFFEDAFPDLPTEGVTSLVDFL TSEEVVCHVARNLAVEQLALSAEFPVPPPVLRQTFFAVIGALLQSSGPERTALFIRDFLITQMTGKELFEMWTITNPMGL LVEELKKRKISAPESRLTRQSGSTTALPVYFVGLYCDRKLIAEGPGETVLVAEEEAARVALRKLFGFTENRRPWDYSKPK EHVRAEKTITAS F1SR64 Mitochondrial ribosomal protein L45 Sus scrofa 0.035331805 1 LEVO MAAPVTRGLSCLPRVLGWWSRQPVLVTQSTAVVPVRTKKRFTPPTYQPKYKSEKEFVEHARKAGLVIPHERLERPIHLAC TAGIFDAYVPPEGDARISSLSKEGLAQRAERLKKNVASQLSIRKIRESDPNFKIKDFPEKAKDIFIEAHLCLNNSDHDRL HTLVTENCFPDMVWDIRYKTVRWSFVESLEPPQVVQVRCSSLMNQGNIYGQVTVRMHTRQTLAIYDRFGRLMYGQEDVPR DVLEYVVFEKHLVDPYGSWRMHGKIIPPWAPPKQPILKTVMIPGPQLKPWEEFEEPQGEVHKPQPA I3LGM5 Mitochondrial ribosomal protein L46 Sus scrofa 0.032215997999999996 1 LEVO MAAPVRRTMLRVVRGWRRFEGPWAHSLGSRNLALAVAPSSSSSPWRLLGALCLQRPPLVTKPLTPLQEEMADLLQQIEIE RSLYSDHELRALDEAQQLAKKKSDLYEEEDEQNILLAQDLEDMWEQKFLHFKLGARLTEADKKDDRTSLHRKLDRNLILL VREKLGDQDIWMLPQSDWQPGETLRQTAERTLATLSENNMEAKFLGNAPCGHYKFKFPQAMRTETSLGAKVFFFKALLLT GDFSQAGKKGHHVWVSKEELGDYLKPKYLAQVRRFLLDL F1SRT0 39S ribosomal protein L48, mitochondrial Sus scrofa 0.029942523 1 LEVO MSEAAGTKVLRELNASRSDIVTGPDQGRSVSIFRPPGIAAAWADLQLARLHTTRQQRMNGALGKALCLRNDTVLKQALSL IRVRASGESPICSAGGILLSTSRHYRSKPTHGIGRYKHLVKAQEPKKKKGKVEVRPINLGTDYEYGVLNIHLIAYDMALA ESYAQYVHNLCNHLAIKVEESYAMPTKTMEVLQLQEQGSKMFLDAVLTTHERVVQISGLSATFAEIFLEIIHSNLPEGVK LSVREHTEEDFKGRFKARPELEELLAKLN A0A480J9Z6 Mrpl34 Sus scrofa 0.018957778999999998 1 LEVO MAATVLCGVLRAWRTGVPLGCGLRRLSQTQGTPEYPSFVESVDEYHFVERLLPPASIPRPPKHEHYPTPSGWQPPRDPAP SLPYFVRRSRMHNIPVYRDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRVKGYFDQQLKAW LLEKGF A0A0R4J8D6 Mitochondrial ribosomal protein L50 Sus scrofa 0.022785357 1 LEVO MVRLRGKRECLTLSLRLIGWSWRVTAGGGRCFVSKFSSKMAARWVSGLARRSLTCAVSGAPRREFWSPFRKEKQPVVAET VEEVKKEPILVCPPIQSRTYIPPEDLQSRLESHVKEVFGSSVPSNWQDISLEDVHLKFSFLARLANDLRHAVPNSRLHQM CRVRDVLDFYNVPVQDRSKFDELIASNLPHNLKITWGY I3L9H6 Mitochondrial ribosomal protein L51 Sus scrofa 0.015249048 1 LEVO MAGSLSWVTGRGLWGRLPLTCRSFSLGIPRLFHVRVTLPPRKVVDRWNEKRAMFGVYDNIGILGNFEKHPKELIKGPIWL RGWKGNELQRCIRKKRMVGNRMFIDDLHNLNKRISYLYKHFNRHGKYR A0A287BE26 Uncharacterized protein Sus scrofa 0.013813704999999999 1 LEVO MAAWGILLSTGVRRLHCGTAAQAGSQWRLQQGFAANPSGYGPLTELPDWSYADGRPAPPMKGQLRRKAQREKFARRVVLL SQEMDAGLQAWQLRQQEKLQEEEGKQKNALKSKGALLQNPQPSQ F1S9B7 mL53 Sus scrofa 0.012015746999999998 1 LEVO MAAALARLGLRAVKQVRVQFCPFEKNVESTRTFLQAVSSEKVRCTNLNCSVIADVRHDGSEPCVDVLFGDGHRLIMRGAH LTAQEMLTAFASHIQARGAAGSGDKPSASTGR A0A341D604 Uncharacterized protein Sus scrofa 0.015957357

residues 54-64 built as poly-Ala

1 LEVO MAARRLFGAARSWAAWRAWELSDAAVSGRLHVRNYAKRPVIKGGKGGKGAVVGEALKDPEVCTDPFRLTTHAMGVNIYKE GQDVVLKPDSEYPEWLFEMNVGPPKKLEELDPETREYWRLLRKHNIWRHNRLSKNRKF I3LN63 Mitochondrial ribosomal protein L57 Sus scrofa 0.012001915 1 LEVO MFLTALLRRNRIPGRQWIGKHRRPRPVSAQAKQNMIRRLETEAENQYWLSRPFLTAEQERGHAAVRRAAAFQALKAAQAA RFPAHRRLEEQLGHLLVTRKWS A0A287BP93 Uncharacterized protein Sus scrofa 0.023545873

residues 165-173 built as poly-Ala

1 LEVO MAAARCLRWGLNRAGAWLLPSPTRYPRRALHKQVEGTEFQSIYSLDKLYPESRGSDTAWRLPDDAKQANDIPVDRLTISY CRSSGPGGQNVNKVNSKAEVRFHLASADWIAEPVRLKLAVKHKNRINRSGELILTSECSRYQFRNLADCLQKLRDMIAEA SQPAKEPSREDAELRRSRIENMNRERLRKKRISSAIKTSRRVDVD A0A286ZJR2 Uncharacterized protein Sus scrofa 0.025815031 1 LEVO MAAPVQQARTLLRLVTTLGQGSRGYRARPPPRRSQEPRWPDPDDPLTPRWQLSPRYAAKQFARHGAASGVAAGSLWPSQE QLRELEAEEREWYPSLAAMQESLRVQQLAEEQKRQAREQLIEECMAKMPQMIENWRQQQQERREKEQADKDRRARLQAEA QERLGYHVDPRSARFQELLQDMEKQHRKRLKEEKQRKKKEARAAAMAAAAAQDPADSETPSS A0A286ZXA6 Mitochondrial ribosomal protein L27 Sus scrofa 0.015943688 1 LEVO MALAVLALRTRAAVTALLSPPQAAALAVRYASKKTGGSSKNLGGKSPGKRFGIKKMEGHYVHAGNILATQRHFRWHPGAH VGLGKNKCLYALEEGVVRYTKEVYVPNPSNSEAVDLVTRLPQGAVLYKTFVHVVPAKPEGTFKLVAML F1RT79 39S ribosomal protein S30, mitochondrial Sus scrofa 0.049100503999999996 1 LEVO MAAARCWRFPLRGAGLSLHTAAKAAVTAPEVTGPDVPATPVARYPPIVASLTAKSKAARQRRVEQWQATVHAAKSVDEKL RILTKMQFMKYVVYPQTFALNADNWYQSFTKTVFLSGLPPTPAKLEPEPTLDITALREAVCDCLLQEHFFLRRKKRAPVI QDREAIASPFLDQLVASLTGLLSVHNPVLAAAALDCKRPVHFFWLRGEEIIPRGHRKGRVDALRYQINDKPHNQIRISRQ LPEFVPLDYSIPIEVPVMSCKPDKLPLFKRQYENTIFIGSKTADPLCYGHTQFHLLPDKLKREKLLKQNCADQIEVVFRA NAIASLFAWTGAQAMYQGFWSEADVTRPFVSQGVITDGKYFSFFCYQLNTLALTAQADQNNPRKNICWGTQSKPLYETIE DNNVKGFNDDVLLQLVQFLLNRPKEDKSQLLEN A0A480QIM3 Mitochondrial ribosomal protein S18A Sus scrofa 0.022273084 1 LEVO MVGLNVLVSGCGRLLRGLLAGPAATSWARPPSRGFREVVEILDGKTTIIEGRITETPQESPNPPNPTGQCPICRWNLKHK YNYEDVLLLSQFIRPHGGMLPRRITGLCQEEHLKIEECVKMAHRAGLLPNHRPKLPEGFVPKSKPRLNRYLTRWSPRSVK PIYNKGHRWNKVRMPVGSPLLKDNVSYSGRPLLLYH F1RRH6 12S rRNA Sus scrofa 5.350865 2 GUUAAUGUAGCUUAAAUUAUCAAAGCAAGGCACUGAAAAUGCCUAGAUGAGCCUCACAGCUCCAUAAACACACAGGUUUG GUCCUGGCCUUUCUAUUAAUUCUUAAUAAAAUUACACAUGCAAGUAUCCGCGCCCCGGUGAGAAUGCCCUCCAGAUCUUA AAGAUCAAAAGGAGCAGGUAUCAAGCACACCUAUAACGGUAGCUCAUAACGCCUUGCUCAACCACACCCCCACGGGAAAC AGCAGUGAUAAAAAUUAAGCCAUGAACGAAAGUUUGACUAAGUUAUAUUAAUUAGAGUUGGUAAAUCUCGUGCCAGCCAC CGCGGUCAUACGAUUAACCCAAAUUAAUAGAUCCACGGCGUAAAGAGUGUUUAAGAAAAAAAAUCACAAUAGAGUUAAAU UAUAACUAAGCUGUAAAAAGCCCUAGUUAAAAUAAAAUAACCCACGAAAGUGACUCUAAUAAUCCUGACACACGAUAGCU AGGACCCAAACUGGGAUUAGAUACCCCACUAUGCCUAGCCCUAAACCCAAAUAGUUACAUAACAAAACUAUUCGCCAGAG UACUACUCGCAACUGCCUAAAACUCAAAGGACUUGGCGGUGCUUCACAUCCACCUAGAGGAGCCUGUUCUAUAAUCGAUA AACCCCGAUAGACCUUACCAACCCUUGCCAAUUCAGCCUAUAUACCGCCAUCUUCAGCAAACCCUAAAAAGGAACAAUAG UAAGCACAAUCAUAGCACAUAAAAACGUUAGGUCAAGGUGUAGCUUAUGGGUUGGAAAGAAAUGGGCUACAUUUUCUACA UAAGAAUAUCCACCACACGAAAGUUUUUAUGAAACUAAAAACCAAAGGAGGAUUUAGCAGUAAAUCAAGAAUAGAGUGCU UGAUUGAAUAAGGCCAUGAAGCACGCACACACCGCCCGUCACCCUCCUCAAGCAUGUAGUAAUAAAAAUAACCUAUAUUC AAUUACACAACCAUGCAAGAAGAGACAAGUCGUAACAAGGUAAGCAUACUGGAAAGUGUGCUUGGAUUACCAAAGCAUAG CUUAAACUAAAGCACCUAGUUUACACCUAGAAGAUCCCACAAUGUAUGGGUACUUUGAACCAAAGCUAGCUCAACAUACU AAACAAAUACAAAAAUACACCAAAAUAAAAUAAAACAUUCACCUAACAUUAAAGUAUAGGAGAUAGAAAUUUUUAUCCUG ACGCUAUAGAGAUAGUACCGUAAGGGAAAGAUGAAAGAAUAAAAUAAAAGUAAAAAAAAGCAAAGAUUACCCCUUCUACC UUUUGCAUAAUGGUUUAACCAGAAAAAAUCUAACAAAGAGAACUUUAGCUAGAUACCCCGAAACCAGACGAGCUACCCAU GAGCAGUUUAAAAGAACCAACUCAUCUAUGUGGCAAAAUAGUGAGAAGACUUGUAGGUAGAGGUGAAAAGCCUAACGAGC CUGGUGAUAGCUGGUUGUCCGAGAAAGAAUUUUAGUUCAACCUUAAAAAUACCCCAAAAACCCUAAAUUCCAAUGUAUUU UUAAGAGAUAGUCUAAAAAGGUACAGCUUUUUAGAAACGGAUACAACCUUGACUAGAGAGUAAAAUCUUAAUACUACCAU AGUAGGCCUAAAAGCAGCCAUCAAUUGAGAAAGCGUUAAAGCUCAACAAAUUCACCAACAUAAUCCCAAAAACUAAUAAC AAACUCCUAGCCCAAUACCGGACUAAUCUAUUGAAACAUAGAAGCAAUAAUGUUAAUAUGAGUAACAAGAAGCCUUUCUC CUCGCACACGCUUACAUCAGUAACUAAUAAUAUACUGAUAAUUAACAACCAAUAAACCAAAACAACACUAAAACGUUUAU UAAUUACAUUGUUAACCCAACACAGGAGUGCACCAAGGAAAGAUUAAAAGAAGUAAAAGGAACUCGGCAAACACAAACCC CGCCUGUUUACCAAAAACAUCACCUCUAGCAUUACUAGUAUUAGAGGCAAUGCCUGCCCAGUGACACCAGUUUAACGGCC GCGGUAUUCUGACCGUGCAAAGGUAGCAUAAUCACUUGUUCUCCAAAUAAGGACUUGUAUGAAUGGCCACACGAGGGUUU UACUGUCUCUUACUUCCAAUCAGUGAAAUUAACCUUCCCGUGAAGAGGCGGGAAUAAAAAAAUAAGACGAGAAGACCCUA UGGAGCUUUAAUUAACUAUUCCAAAAGUUAAACAACUCAACCACAAAGGGAUAAAACAUAACUUAACAUGGACUAGCAAU UUCGGUUGGGGUGACCUCGGAGUACAAAAAACCCUCCGAGUGAUUUUAAUCUAGACAAACCAGUCAAAAUAACCAUAACA UCACUUAUUGAUCCAAAAUUUUGAUCAACGGAACAAGUUACCCUAGGGAUAACAGCGCAAUCCUGUUCUAGAGUUCCUAU CGACAAUAGGGUUUACGACCUCGAUGUUGGAUCAGGACACCCAAAUGGUGCAACCGCUAUUAAAGGUUCGUUUGUUCAAC GAUUAAAGUCCUACGUGAUCUGAGUUCAGACCGGAGCAAUCCAGGUCGGUUUCUAUCUAUUAUAAAUUUCUCCCAGUACG AAAGGACAAGAGAAAUGGGACCAACCUCACAAACGCGUCUCAGAGAUAAUUAAUGAUUUAAUCUUAACCUAAUUAACUCA UAAUAAAUCCAGCCCUAGAACAGGGCACAUUAGGGUGGCAGAGACCGGUAAUUGCGUAAAACUUAAACCUUUAUUACCAG AGGUUCAACUCCUCUCCCUAAUAACAUGUUCAUAAUUAAUAUUCUAAGCCUAAUUAUUCCUAUCCUACUGGCCGUAGCAU UCCUCACCCUAGUAGAACGAAAAGUGCUAGGUUAUAUGCAACUACGAAAAGGACCCAACGUUGUAGGCCCCUACGGCCUA CUCCAACCCAUCGCCGAUGCCCUAAAACUAUUCACCAAAGAACCCCUACGACCAGCCACAUCCUCAAUCUCCAUGUUCAU UAUUGCACCAAUCCUAGCCUUAUCCCUAGCACUAACAAUAUGAGUUCCACUACCAAUACCCUACCCUCUAAUCAACAUAA ACCUAGGAGUACUAUUCAUGCUAGCCAUGUCAAGCCUAGCAGUCUACUCUAUCCUAUGAUCAGGAUGAGCAUCCAACUCA AAAUACGCACUCAUCGGGGCCCUACGAGCAGUAGCCCAAACAAUCUCAUAUGAAGUAACACUAGCAAUCAUCCUACUAUC AGUACUCCUAAUAAAUGGAUCAUAUACUCUAUCAACCCUAAUCACAACACAAGAGCACAUUUGAAUAAUCUUUACAUCCU GACCCCUAGCCAUAAUAUGAUUUAUCUCAACCCUAGCAGAAACCAACCGAGCCCCGUUCGACCUUACAGAAGGAGAGUCA GAACUUGUAUCAGGCUUUAACGUAGAAUAUGCAGCCGGACCUUUCGCCAUAUUCUUCAUAGCAGAAUAUGCCAACAUCAU CAUAAUAAAUGCAUUUACAGCAAUUCUCUUCCUAGGAGCAUCCCACGACCCACACACACCAGAACUAUAUACAAUCAACU UCGUACUAAAAACACUCGCAUUAACAAUCACCUUCCUAUGAAUCCGAGCAUCAUACCCACGAUUCCGAUACGACCAACUA AUACAUUUACUAUGAAAAAGCUUCCUGCCCCUAACACUAGCUCUAUGUAUAUGACACAUCUCACUCCCUAUUAUAACAGC AAGCAUUCCCCCACAAUCAUAGAAAUAUGUCUGAUAAAAGAGUUACUUUGAUAGAGUAAAAAAUAGAGGUUCAAACCCUC UUAUUUCUAGAACAAUAGGACUCGAACCUAAACCUGAGAAUUCAAAAUUCUCCGUGCUACCAAAAUACACCACAUUCUAC AGUAAGGUCAGCUAAGCUAAGCUAUCGGGCCCAUACCCCGAAAAUGUUGGUUCAUACCCUUCCCAUACUAAUUAAUCCCA UUAUCUACACUACCCUUAUCAUAACAGUAAUGUCCGGAACCAUACUAGUAAUAAUCAGCUCACACUGACUACUCAUCUGA AUCGGAUUCGAAAUAAACCUAUUAGCAAUAAUCCCAGUAUUAAUAAAAAAUUUUAACCCACGAGCCACAGAAGCAGCCAC AAAAUAUUUCCUAACACAAGCCACAGCCUCCAUAAUACUAAUAAUAGCCAUCAUCAUCAACCUCCUAUAUUCUGGCCAAU GGACCAUUACAAAAAUAUUUAACCCAGUAGCAAUAACAAUAAUAACCAUGGCCCUAGCCAUAAAACUAGGACUCUCACCU UUCCACUUCUGAGUCCCAGAAGUAACCCAAGGCAUUUCACUACAAGCAGGCCUACUGUUACUAACAUGACAAAAACUAGC CCCAUUAUCAGUACUAUGCCAAAUCUCACAAUCAAUCAACCCAAACCUAAUACUAACUAUAGCCAUAUUAUCAAUUUUAA UCGGAGGGUGAGGAGGGCUAAAUCAAACCCAACUUCGAAAAAUCAUAGCAUACUCAUCAAUCGCACACAUAGGAUGAAUG ACAGCAGUAUUACCAUACAACACAACCAUAACAAUCUUAAACCUACUAAUUUACAUCACAAUAACACUAGCAAUAUUCAU ACUAUUAAUCCACAGCUCAGCAACCACAACUUUAUCCCUAUCCCAUACAUGAAACAAAAUACCCGUCAUCACAAGCCUAA UAAUAGUAACCCUACUCUCAAUAGGAGGCCUGCCUCCACUAUCAGGAUUUAUGCCAAAAUGAAUAAUUAUUCAAGAAAUA ACAAAAAAUGAAAGCAUCAUCAUGCCAACACUCAUAGCAAUAACAGCACUGCUAAACCUCUAUUUCUACAUACGACUAGC CUACUCCUCCUCACUGACUAUGUUCCCAUCCACCAACAACAUAAAAAUAAAAUGACAAUUCGAACACACAAAACAAAUAA AAUUACUUCCCACAAUAAUUGUAUUAUCAACACUAGUCCUACCUAUAACACCAGCCCUCUCGUCCCUAAACUAGGAAUUU AGGUUAACACAGACCAAGAGCCUUCAAAGCUCUAAGUAAGUACAAAGUACUUAACUCCUGAAAACCUAAGGACUGCAGGA CUUAUCCUACAUCAAUUGAAUGCAAAUCAAACACUUUAAUUAAGCUAAAUCCUCACUAGAUUGGUGGGAUUACAUACCCA CGAAACUUUUAGUUAACAGCUAAACACCCUAAUCAACUGGCUUCAAUCUACUUCUCCCGCCGCAGGAAAAAAAAGGCGGG 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CAGACAUAGACAAAAUUCCAUUUCACCCAUACUACACUAUUAAAGACAUUCUAGGAGCCUUAUUUAUAAUACUAAUCCUA CUAAUCCUUGUACUAUUCUCACCAGACCUACUAGGAGACCCAGACAACUACACCCCAGCAAACCCACUAAACACCCCACC CCAUAUUAAACCAGAAUGAUAUUUCUUAUUCGCCUACGCUAUUCUACGUUCAAUUCCUAAUAAACUAGGUGGAGUGUUGG CCCUAGUAGCCUCCAUCCUAAUCCUAAUUUUAAUGCCCAUACUGCACACAUCCAAACAACGAGGCAUAAUAUUUCGACCA CUAAGUCAAUGCCUAUUCUGAAUACUAGUAGCAGACCUCAUUACACUAACAUGAAUUGGAGGACAACCCGUAGAACACCC GUUCAUCAUCAUCGGCCAACUAGCCUCCAUCUUAUACUUCCUAAUCAUUCUAGUAUUGAUACCAAUCACUAGCAUCAUCG AAAACAACCUAUUAAAAUGAAGAGUCUUCGUAGUAUAUAAAAUACCCUGGUCUUGUAAACCAGAAAAGGAGGGCCACCCC UCCCCAAGACUCAAGGAAGGAGACUAACUCCGCCAUCAGCACCCAAAGCUGAAAUUCUAACUAAACUAUUCCCUGCAACC AAAACAAGCAUUCCAUUCGUAUGCAAACCAAAACGCCAAGUACUUAAUUACUAUCUUUAAAACAAAAAAACCCAUAAAAA UUGCGCACAAACAUACAAAUAUGUGACCCCAAAAAUUUUACCAUUGAAAACCAAAAAAUCUAAUAUACUAUAACCCUAUG UACGUCGUGCAUUAACUGCUAGUCCCCAUGCAUAUAAGCAUGUACAUAUUAUUAUUAAUAUUACAUAGUACAUAUUAUUA UUGAUCGUACAUAGCACAUAUCAUGUCAAAUAACUCCAGUCAACAUGCAUAUCACCACCACUAGAUCACGAGCUUAAUUA CCAUGCCGCGUGAAACCAGCAACCCGCUUGGCAGGGAUCCCUCUUCUCGCUCCGGGCCCAUAAACCGUGGGGGUUUCUAU UGAUGAACUUUAACAGGCAUCUGGUUCUUACUUCAGGACCAUCUCACCUAAAAUCGGCCACUCUUUCCCCUUAAAUAAGA CAUCUCGAUGGACUAAUGACUAAUCAGCCCAUGCUCACACAUAACUGAGGUUUCAUACAUUUGGUAUUUUUUAAUUUUUG GGGAUGCUUAGACUCAGCCAUGGCCGUCAAAGGCCCUAACACAGUCAAAUCAAUUGUAGCUGGACUUCAUGGAACUCAUG AUCCGGCACGACAAUCCAAACAAGGUGCUAUUCAGUCAAUGGUUACGGGACAUAACGUACAUACACGUGCGUACACGUGC GUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGC GUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGC GUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGCGUACACGUGC GUACACGUGCGUACACGUGCGUACACGUGCGUACACGCGCAUAUAAGCAGGUAAAUUAUUAGCUCAUUCAAACCCCCCUU ACCCCCCAUUAAACUUAUGCUCUACACACCCUAUAACGCCUUGCCAAACCCCAAAAACAAAGCAGAGUGUACAAAUACAA UAAGCCUAACUUACACUAAACAACAUUUAACAACACAAACCACCAUAUCUUAUAAAACACUUACUUAAAUACGUGCUACG AAAGCAGGCACCUACCCCCCUAGAUUUUUACGCCAAUCUACCAUAAAUAAAUUUAAAAUUACAACACAAUAACCUCCCAA AAUAUAAGCACCUAUUUAAGUAUACGCCCACAAUCUGAAUAUAGCUUAUA JN601068.1 Mitochondrial ribosomal protein S2 Sus scrofa 0.031731604 1 LEVO MASGPLLPRLLGAGAPRRPLRLHLVPTATPGPAGPTGRTLGSNAAPAAREPELGNDLRDRILSEPLKHADFFNLKELFSV RSLFDARVHLGHKAGCRHRFMEPYLFGSRLGQDIIDLEQTAAHLQLALNFTAHVAYREGIILFVSRHRQFAHLIETTARD CGEYAHTRYFKGGLLTNAPLLLGPGVRLPDLIIFLHTLNNVFEPHVAVRDAAKMNIPTVGIVDTNCNPALITYPVPGNDD SPPAVRLFCRLFQVAISRAKEKRRQVEALYRLQGPAGAERGPAAPPPPE F1S001 Mitochondrial ribosomal protein S24 Sus scrofa 0.018935171999999997 1 LEVO MVASVCGGGLRPLVLSWSRELPCAWRALHISAVCAKNRAARVRVSKGDKPVTYEEAHAPHYIAHRKGWLSLHTGNLDGED HAAERTVEDVFLRKFMLGTFPGCLADQLVLKRRANQLEICALVLRQLPPHKFYFLVGYSETLLSHFYKCPVHLHLQTVPS KVVYKYI A0A287ANU4 Mitochondrial ribosomal protein S5 Sus scrofa 0.048213074 1 LEVO MAAAVRAAGFFPGLFGASAGNLWSRQLYLNTFPAASILTSKTFPSNGPLSPPGTRDNHHFTSLACALQTQCSISSPSNWM GQQYRSYSFFTKLTADELWKGALAESGAGARKGRGKRTKKKRRKDLNRGQIIGEGRHGFLWPGLNIPLMRNGAVQTIAQR SKEDQEKVEADMVQQREEWDRRRKMKVKRERGWSGNTWGGVSLGPPDPGPNGETYDDFDTRILEVRNVFNMTAKEGRKRS VRVLVAVGNGKGAAGFAIGKATERADAFRKAKNRAVHYLHYIERYEDHTIYHDISLKFKRTHIKMKKQPRGYGLHCHRAI MTICRLIGIKDLYAKVSGSVNMLNLTRGLFLGLSRQETHQQLADKKSLHVVEFREECGPLPIVVASPQGALRKDPEPEDE VPDITLDWEDVKAAQGMKRSVWSGLKRAAT F1SU66 Uncharacterized protein Sus scrofa 0.030365266 1 LEVO MRRKAWKVPAGSRTIRSKRGGSGSCRVGGVCSSRWQLAEGTRGAALLQWLPGQGAGPRAGCRPQRAGLSRGRGAAALRRG RGVARGGAWLGEGAWRPVCAAARAFKPGLARPERAFAAWEPSGPAVRQVSAVPALFHPGRRFREEPRAVHSGMPRYELAL ILKAMQRPETAAALKRTLEALMDRGAVVRNLENLGERMLPYKISAHNQRHSRGGYFLVDFYAPATTVESMMEHLSRDIDV IRPNIVKHPLTQEVKECEGIVPVPLEEKLYSTKKRK I3LEJ9 Mitochondrial ribosomal protein S7 Sus scrofa 0.028150918 1 LEVO MAAPAVKAARGWSCSALGVRIAVLRFPGLTQVRWSRYGPEYKDPQIDKEYYRKPLAEQTEEEKYERDFKKTQLIKAAPAT KTSSVFEDPVISKFTNMMMKGGNKVLARSLMTQTLEAVKRKQFAKYHAASAEEQATIERNPYTIFHQALKNCEPVIGLVP ILKGGHFYQVPVPLADRRRRFLAMKWMIAECREKKHRRVLMPEKLSQELLEAFHNQGPVIKRKHDMHKMAEANRALAHYR WW A0A286ZJ25 Mitochondrial ribosomal protein S10 Sus scrofa 0.023105677999999998 1 LEVO MAARTAFGALARRLWQGSRNFSVSSSRSSTAKNGGFLLTTMKWVQFSNLHVDVPKDMTKPEITISDEPDTLYKRLSVLVK GHDKAVLDSYEYFAVLAAKELGISIKVHEPPRKIERFTLLKSVHIFKKHRVQYEMRTLYRCLELEHLTGSTADVYLEYIQ RNLPEGVAMEVTKTKLEQLPEHIRKPIWETMPEEKEESKS F1RUU2 uS11m Sus scrofa 0.020841922 1 LEVO MQVVRNAGSRLLQSWAWPPKTRIVAGLPASTIHRSAPHLQDAAAKLDIEDKEEARTPSHSSFSIYPPIPGQENSLRWAGK KFEEIPIAHIKASYNNTQIQVVSAAHQPLAHASCGTEGFRNAKKGTGIAAQTAGIAAAAKATGKGVTHVRVVVKGLGPGR LSAIKGLTMGGLEVISITDNTPIPHNGCRPRKARRL A0A286ZJJ6 Mitochondrial ribosomal protein S12 Sus scrofa 0.015257106999999999 1 LEVO MSWSGLLRGLGTSLNCGLALVPRPWATRPMATLNQMHRLGPPKHPPGKMGPTAGRPQLKGVVLRTFIRKPKKPNSANRKC CRVRLSTGREAVCFIPGEGHSLQEHHVVLVQGGRTQDLPGVKLTVVRGKYDCGHVQKKK A0A0M3KL52 Mitochondrial ribosomal protein S14 Sus scrofa 0.015120739 1 LEVO MAASMLGFLLRTVRQMVPSSASGQVRSYYVDWKMLRDVKRRKMAYEYADERLRINSLRKNTILPKDLQEVADEEIASLPR DSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGVQRAMW A0A0M3KL53 uS15m Sus scrofa 0.027411427999999998 1 LEVO MLRAAWRALSSIRTQAVTQAPVLGLPGGGCARFPSVQRALPSRPAGLILRAARGYAAQKPVQPNQDDPPPSMLLLDYQNV PGIHKVDDVVKRLLSLEMANQKEKLKIKKMQLMNKVLENPEDTSSLEARIVALTVKIRNYEEHMQKHRKDKAHKRFLLMS IDQRKKMLKNLRETNYAVFEKICKELGIEYTFPPPYHRKAHRRWVTKKALCTQVFREVQKLKKQKRALRAAAVAAHKQG A0A287BMP0 28S ribosomal protein S16, mitochondrial Sus scrofa 0.015182667999999998 1 LEVO MVQLTTVLCRAYRGGHLTIRLALGGCTNRPFYRIVAAHSKCPRDGRFVEQLGSYDPLPNSHGEKLVALNFDRIRHWIGCG AHLSKPVEKLLGLSGFYPLHPMMITNAERLRRKRAREVLLASQKTDTEATETKAS A0A480JTD7 uS17m Sus scrofa 0.014452014 1 LEVO MSIARSSVHAKWVVGKVIGTAMQKTAKVRVTRLVLDPYLLKYFNKRKTYFAHDALQQCTVGDIVLLKALPVPRTKHVKHE LAEIIFKVGQVIDPVTGKPCAGTTYLESPISPESTRLTKNLEALQLSSTQ F1RIU0 Mitochondrial ribosomal protein S18C Sus scrofa 0.016211086 1 LEVO MAAVVSLCSRLGRRKSTDFVNAAVCLTDPGTHAVLWRSCSQYKQVASNEDLPVPMENPYKEPLKKCILCEKHVDYKNVQL LSQFISPFTGCIYGRHITGLCGKKQKEITKAIKRAQILGFMPVTYKDPAYLKDPKVCNIKYRE A0A0M3KL54 bS21m Sus scrofa 0.010682506 1 LEVO MANHLKFIARTVMVQEGNVEGAYRTLNRILTMDGLIEDIKRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNR ADPWQGC A0A286ZNB3 tRNAMet Homo sapiens 0.022664497999999998 2 AGUAAGGUCAGCUAAAUAAGCUAUCGGGCCCAUACCCCGAAAAUGUUGGUUAUACCCUUCCCGUACUACCA KY671085.1 mRNA Homo sapiens 0.0018921729999999998 1 CUGAUG unknown peptide Sus scrofa 0.0012974159999999998 1 LEVO AAAAAAAAAAAAAAAAAA Mitochondrial ribosomal protein S22 Sus scrofa 0.043234644999999995 1 LEVO MPHEPRETRNLSLPLPALVARIIMATLKASALLRSLQTNSCGTGRVCFPVRARPRPRALLQPLPGASGTGTLCRGLGSES ESGNSEIRKPTFMDEEVQNILIKMTGLDLQKIFKPALQELKPPTYKLMTQAQLEEATKQAVEAAKVRLKMPPVLEERAPI NDVLAEDKILEGTETAKYVFTDISYSIPHRERFIVVREPSGTLRKASWEERDRMIQVYFPREGRRILTPVIFKEENLQTM YSQDQHVDVLNLCVAQFEPDSAEYIKIHHHTYEDIDKCGKYDLLRSTRHFGGMAWYFVNKKKIDGLLIDQIQRDLVSDAT SLVHLYHILHPDGQSAQEAKKQGAEGLHLIKVFAKTEAQKGAYIELTLQAYQEAFITHSAAS A0A287AQT5 mS23 Sus scrofa 0.021666673999999997 1 LEVO MAGSRLETVGSIFSRTRDLIRAGVLKEKPLWLDIYNAFPPLREPVFRRPRLRYGKAKAAVQDIFYHEDRIRAKFYSAYGS GPKAFDLFNPNFKSTCQRFVEKYIELQRLGETDEEKLFVEAGKALLAEGVTLRRVGEARTQQEGSPISRKSEPTGIKPQT AVEENQPWKEVPQDQPLEAPEKESKGLPPP A0A287AEW3 Mitochondrial ribosomal protein S25 Sus scrofa 0.019954186000000002 1 LEVO MPMKGRFPIRRTLQYLGQGDVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQITMFKNMTPSPFLRFYL DSGEQVLVDVESKSNKEIVEHIRKILGKNEETLEKEEQEKKQLSHPAHFGPRKYCLRECMCEVEGQVPCPGLVPLPKEMT GKYKAALKATAQD A0A0M3KL55 Mitochondrial ribosomal protein S26 Sus scrofa 0.0236379 1 LEVO MLRVLGGLGARPAGRLPAPLLLPTRGRKTRHDPPAKSKAGRVATPPAVDPTEFFVLTERYRQYRQTVRALRLEFMSEVRK KLHEARAGVQAERKAQEDAAEHRELMAWNQAENQRLHELRLARLRQEALEQERRQAEEAVLQAREAQAWAQLKEQEVLQL QEEAKTFITRENLEARVEEALDSPKSYNWAITREGLVVRPQQKGS A0A287AY01 Mitochondrial ribosomal protein S27 Sus scrofa 0.051945983999999994

residues 337-352 built as poly-Ala

1 LEVO MLSRAAPTEGTGGGRRSVDSTPPPRLPATPAPAFPLVCSKMASSIVRRGILRARKVSLPQLSLAGRRCLLSAAYVDSHKW EARDKEDCHLADLASLMDKTYERKLPVSSLTISRFVDNIASREDIDHAEYYLYKFRHSPNCWYLRDWTIHTWIRQCLKYG AQDKALYTLKNKVQYGIFPDNYTFNLLMDYFIKKENYKDALSVVFEIMMQEAFEVPSTQLLSLYVLYHCLAEKTDLSWEE ERNFGASLLLPGLKQKNSVGLSSQLYGYALLGKVELQQGLRAVYCNMPLLWQPGYLHRALQVMEKVASSPEDGQLCGEAL DVLDRVLKAVTAPAQGTSEEQPQEGEESQRSEELVEQLDVEETEQSKLPQYLERFEALRSKLQALGKVESESLLTLSTQL AKAQLPTCEAEDIAVYEQRLQQWHGERVRLIQREQEQRERARQDYEARRAAKASA A0A5G2R5D6 Mitochondrial ribosomal protein L28 Sus scrofa 0.030148953 1 LEVO MPLHKVPVGLWKQLRLWEGIYSRLPRHYLRSLEEARTPTPVHYRPHGAKFKINPKNWQRERVEDVPIPVHYPPESQLGLW GGEGWVLGHRYVNNDKLSKRVRKVWKPQLFQRELYSEILDKRFTVTVTMRTLDLIDQACGFDFYILKTPKEDLCSKFGMD LKRGMLLRLARQDPQLHPDDPARRAAIYDRYKAFVIPEAEAEWVGLTLDEAVEKQRLLEEKDPIPLFKIFVEELLGQLQQ QALSEPAVVQTRASRK A0A286ZTW8 Mitoribosomal protein ms28, mrps28 Sus scrofa 0.021014912 1 LEVO MAALCRTRAVTAKSHFLRVFFFSRPCRGTGTESGSGSESSESTEPKQRPGGFASALERHSELQQKAEFGRMRGSPKNVES FASMLRHSPLTQMGPAKNKLVIGQIFHIVEDDLYIDFGGKFHCVCKRPEVDGEKYQKGTRVRLRLLDLELTSRFLGATTD TTILEADAVLLGLQESKDSKSKEERHEK A0A0M3KL56 Death associated protein 3 Sus scrofa 0.046966061999999996 1 LEVO MAQLLANLTSIQVMLKGVTRLISRVHKLDPGHVLYMGTQAPQSLAAHLDNQVPVKSPRAISRTSEDDPAKHREQHEGQHY NISLQELKTVFPHGLPPRFAMQVKTFNEACLMVRKPALELLHYLKNTNFAHPAVRYVLYGEKGTGKTLSLCHILHFCAKQ NWLILHIPDAHIWVKNCRDLLQSNYNKQRFDQPLEASTWLKNFKTANEHFLSQIKVQEKYVWNKRESTEKGRPLGEVVEQ GIMRVRNATDAVGIVLKELKRQSSLGIFHLLVAVDGVNALWGRTTLKREDKSPIAPEELALIHNLRKMVKNDWQGGAIVL TVSQTGSLFKPRNAYLPQELLGKEGFDALDPFIPILVSNYNPKEFESCIQYYLENNWLQHEKAHTEEGKKELLFLSNRNP GQLERLCAYL I3LS10 Mitochondrial ribosomal protein S31 Sus scrofa 0.044091995999999994 1 LEVO MLPRVSAVIPLRPLSRLPLCSGGPEASAAAILLLDSLRGTVRTENNIQRYFGTNNLVYSKKDEQSGPTCEISKETESKGS VKENSKKDLLNIIKGMKVELSTVNVQTTKPSNKRQLKNLETAIGRIKKAPEDTPQKRSESLSSELVAAVSAVADSLPFDR RMTKSELLRQLQKHEEDSR(UNK)QKDGEKPKISFSNIISDMKVARSATARVSTRPVHQIQFDEGTDDFADQEKTLNLKK SFRKNIFKGRRLNIFEPQAVTEEAPETEAVPSLWDIEFAKQLAAVTERPFQNGFEEMIQWTKEGKLWEFPINNEAGFDDD GSEFHEHIFLDKYLQDFPKQGPIRHFMELVTCGLSKNPYLSVKQKVEHIEWFRNYFNEKRVILKESGIQLN F1RME2 mS33 Sus scrofa 0.01254465 1 LEVO MSSLSEYAVRMSRLSARLFGEVARPTDSKSMKVVKLFSEQPLAKRKETYDWYPNHNTYFALMGTLRFLGLYRDEHQDFRD EQLRLKKLRGKGKPRKGEGKRAMKKK F1S8G3 mS34 Sus scrofa 0.025843817999999998 1 LEVO MARKKVRPRLIAELARRVRALREQRERPRDSQRYALDYETMMRPHSGNRLPMRAWADVRHESRLLQLLNRLPLFGLGRLV TRKSWLWQHDEPCYWRLTRVRPDYAAENLDHGKAWGILTFKGKTESEAREIEQVMYHDWRLVPKHEEEAFTAFTLVPEDT PLSVPYPPLLRAMILAQRQKYGDTSTEEPMLNLERARIDPWDYPAKLEAKTKTKGAAV A0A287AVS9 Mitochondrial ribosomal protein S35 Sus scrofa 0.037104809 1 LEVO MAATSLPAWLILKSRAGTFRAFSTAMSPATRSPRPALRTTERTSKPERALRRKALPPRTEKMAVDQDWPSVYPVAAPFKP SAVPLPVRMGYPVKRGVPMAKEGNLELLKIPNFLHLTPVAIKRHCEALKDFCTEWPAALDSDEKCEKHFPIEIDTADYVS AGPSIRNPKARVVTLRVKLSSLNLDDHAKKKLIKLVGDRYCKSTDVLTIKTDRCPLKRQNYDYAVYLLTVLYHESWKTEE WEKKKTEADMEEYIWENSTSEKNILETLLQIKAAEKNLELSKEELLGTKEVEDYRKSVVSLKNEGDNENTLSQYKESVKR LLNLA F1SG95 mS37 Sus scrofa 0.013561903 1 LEVO MATPSLRGRLARLGNPRRPILKPNKPLILANHVGERRREKGEATCITEMSVMMACWKQNEFRDEACRKEIQDFFDCASRA EAARKMRSIQENLGELGSLPPKKLNKLLHRFPNKPHVS F1SU49 Aurora kinase A interacting protein 1 Sus scrofa 0.022843941 1 LEVO MFLVRLTSQLLRAVPGAGRSGSPRVSEVLGRHACRPRYSTQPPGPSGIASLPGKHVHPELEEMLVPRKMSISPLESWLTI RYLLPRLGAGTPGTVSPAQLYQCPPSQVGEGVKQGDKEVRDTPQMQCKNILKIRRRKMNHHKYRKLVKRTRFLRRKVREG RLRQKQVKFERDLKRIWQKAGLKEAPAGWQTPKIYLKNQ A0A287B3R8 mS39 Sus scrofa 0.078518156

residues 201-234 built as poly-Ala

1 LEVO MAAVASARWLGVRSGLCLPLTGRRVGPCGRTPRSRFYSGSAAHPEVEGANVTGIEEVVIPKKKTWDKVAILQALASTVHR DSTAAPYVFQDDPYLIPTSSVESHSFLLAKKSGENAAKFIINSYPKYFQKDIAEPHIPCLMPEYFEPQIEDVSEAALQER IKLRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGNQEPSANYNFQQREQSEELEEATEADNEKSKTKAGHHLGVT WRTKNNAERIFALMPEKNAHSYCTMIRGMVKHQAPTQALNLYTVLLNNRLRADVYTFNSLIEATALVVNEKFEEKWNNIL DLLKQMVTQNVKPNLQTFNTILKCLRRFYAFGKLPALQTLREMKAIGIEPSLATYHYVIQLFYQHESPSKGSSLIIYDIM NEVMGKRFSPRDPDDDMFFQSAMRVCSSLRDLELAYQVHGLLNTGDNWKLIGSDHRRNFYYSKFFNLLCFMEQIDVTLKW YKDLIPSVFFPHSQTMIDLLQALDVANRLDMVPQIWKDSKEYGHTFRNELKEEILMLMARDQHPPELQVAFADCAADIKS TYESQDARQTAPEWPASSLNYVAVLFLRAGRTQEAWKMLGLFRKHNKIPRAELLNEFLDSAKASSSPAQAIELVKLASAF SLPVCEGLTRRVMAEFTLTQEQREALGELTALTSDSSSDSDSDSDSDISEGK K7GKS8 28S ribosomal protein S18b, mitochondrial Sus scrofa 0.029220464999999998 1 LEVO MAASILNVLLRRLPGVSPFRGAYGVQVLLQTLCTKAPPEDDSLPQVPISPYKDEPWKYLDSEEYQNRYGSRPVWADYRRN HKGGIPPQRTRKMCIRGNKVAGNPCPICRDQKLHVDFRNVKLLEQFVCAHTGIIFHAPYTGVCMKQHKKLTQAIQKARDH GLLRYHIPQVEPRDLDFSTTHGAVSSTPPAPTLVSGDPWYPWYSWKQPPERELSRLRRLYQGRLREESGPPPELMPEVPL TAPAEASSTEPGAPQSAL Q767K8 Mitochondrial ribosomal protein L12 Sus scrofa 0.021268975 7 LEVO MLPAAASSLWGPCFGLRAAALRVARHQGPRLCGVRLMRCSSHRKGEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEIS DLNELLKKTLKIQDVGFMPMGAVAPGAPPAAAAPEAAEEDLPKRKEQTHFTVRLTEAKPVDKVKLIKEIKSHIQGINLVQ AKKLVESLPQEIKANVPKAEAEKIKAALEAVGGTVVLE A0A287ARV8 Mitochondrial ribosomal protein L47 Sus scrofa 0.030085088 1 LEVO MAAAGLAVFCRRVSAALKACRLLIRPQAPPSTSCRFSPSLLPKNTPNVTSFHQFRIFHTTFSRRGLEEFFDDPKNWGEEK VKSGASWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLEKVVDSMDALDKVVQEREDALRLLQT GQEKARPGAWRRDIFGRIIWHKFKQWPIPWYLNKKYNRKRFFAMPYVERFVRMRIEKQARIKARKRSLERKKEKFLQEKF PHLSETQKSSHV F1SGC7 Uncharacterized protein Sus scrofa 0.018921099999999996 1 LEVO HPPRQDFLVRRQPHRNRTVTKGMESLICTDWIRHKFTRSRIPDKVFQPSPEDHEKYGGDPQYPHKLHIVTRIKSTKRRPY WEKDIIKMLGLEKAHTPQVHKNIPSVNAKLKVVKHLIRIKPLKLPQGLPTEEDMANTCLKSNGELVVRWLLNPANQEARK S F1STE5 Uncharacterized protein Sus scrofa 0.015156537

residues 83-96 built as poly-Ala

1 LEVO MAAKGSLLRLLWQRVVTGAAPESCRHLYTSSWRADCNRALLTRLHRQTYARLYPVLLVKQDGSTIHIRYREPRRMLTMPV DLDSLSPEERRARFRKREAKFKEKKEEPELSDDFDVEQYKRFWTKK I3L9J0 Uncharacterized protein Sus scrofa 0.02153793 1 LEVO MASAMLVLVVPPWPAARGLLRNWWEQLQRKLQHNRLGLPLHPWGPALAVQGPAICTEPANDTNGSKAISSLLDSVFWMAA PKNRRSIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHILCGYCYEKVRKETAEIRRQMGKQEGGPFRAPTTETVVLY SGETPSEQDQGKRIIERERKRPSWFTQN I3LTC4 Uncharacterized protein Sus scrofa 0.007508096999999999 1 LEVO MFLSAVTFAKSKSKTILVKMMSQAGTGFSFNTKRSRLREKLTLLHYDPVVKKKVLFVEQKKIRSL A0A286ZU68 Mitochondrial ribosomal protein L34 Sus scrofa 0.010871728999999998 1 LEVO MAFLARSVGRLLDPVSRSAALVGGRWLQPQAWLGFPDTWGLPAMQQTRGKARGNEYQPSNIKRKHKHGWVRRLRTPTGVQ VILRRMHKGRKSLSH A0A287A119 Mitochondrial ribosomal protein L35 Sus scrofa 0.021548543 1 LEVO MAASAFAGAVRAATGILRPLHILASSAYQNCAKNACLSSVLSSRHFSHIQTSALLSAPRLITSVRNLMCGPTAPVLNRVA PLLPHVLKPPVRTVTYFSSRKGKRKTVKAVIYRFLRLHSGLWLRRKAGYKKKLWKKTAARKRRLREFVFCNKTQSKLLDK MTTSFWKRRNWYADDPYQKYQDRTNLKV F1SVC5 Ribosomal protein Sus scrofa 0.010841015999999998 1 LEVO MATAFLRTVLSAVGPLLHLGGRPLSTFAAGPPRAALAVGAQPSPAAALLSARPLLGPQPALGFKTKGVLKKRCRDCYLVK RRGRWFIYCKTNPKHKQRQM A0A287A731 CP tRNA-Phe Sus scrofa 0.023402018 1 GUUAAUGUAGCUUAAAUUAUCAAAGCAAGGCACUGAAAAUGCCUAGAUGAGCCUCACAGCUCCAUAAACACCA DQ207753.1 Elongation factor G, mitochondrial Homo sapiens 0.08005414799999999

mtEFG1 residues 40-751 including an N-terminal SGGSGSGSSG-linker

1 Escherichia coli LEVO GGSGSGSSGVIPNEKIRNIGISAHIDSGKTTLTERVLYYTGRIAKMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTM WKDVNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVNRQMKRYNVPFLTFINKLDRMGSNPARALQQM RSKLNHNAAFMQIPMGLEGNFKGIVDLIEERAIYFDGDFGQIVRYGEIPAELRAAATDHRQELIECVANSDEQLGEMFLE EKIPSISDLKLAIRRATLKRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNKEDDSKEKTKILMNSSRDNS HPFVGLAFKLEVGRFGQLTYVRSYQGELKKGDTIYNTRTRKKVRLQRLARMHADMMEDVEEVYAGDICALFGIDCASGDT FTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLER EYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGF LDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINR RHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAK N Q96RP9 Uncharacterized protein Sus scrofa 0.033270934 1 LEVO MALRVLTRALSSLSLTPRIAVAPGLNLLPAVQVTNNVLLTLPSGLMSLPCRPILTSVALSATSVSWKSRTKYTVMPVKMR KSGGRNHTGQIQVHGIGGGHKQRYRMIDFLRFRPEHESKPGPFEEKVIAVRYDPCRSADIALVAGGNRKRWIIATENMKA GDTVLNSDHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGRGAQYIRAAGTCGVLLRKVNGTAIIQLPSKRQMQVLET CIATVGRVSNVDHNKRVIGKAGRNRWLGKRPNSGLWQRKGGWAGRKIRPLPPMKSYVKLPSAAAQS F1RRN2 ICT1 Sus scrofa 0.038427199 1 LEVO MPGWGLLGRAGARVLGCGADGLGASRGLGNRTDICLLVRSLHGKSVTWWDEHLSEENVPFVKQLVSDENKAQLASKLCPL KDEPWPIHPWEPGSSRVGLIALKLGMMPLWTKDGQKHVVTLLQVQDCHVLKYTPKENHNGRMAALTVGGKTVSHFHKSAS ILEFYQELGLPPKQKVKIFNVTENAVIKPGTPLYAAHFRPGQYVDVTAKTIGKGFQGVMRRWGFKGQPATHGQTKTHRRP GAISTGDVARVWPGTKMPGQLGNIDRTAFGLKVWRINTKHNIIYVNGSVPGHKNCLVKIKDSKLPAYKDFCKNLPFPTYF PDGDEEALPEDLYDENVCQPGAPSITFT A0A140UHW4 Mitochondrial ribosomal protein L4 Sus scrofa 0.033251469 1 LEVO MLQLVRAGARTWFRPSGCRGLNTLAEEAVQQAEKPESVASLGLQPPVLRKCELPVPAHRRPVQAWIESLRGYEQERVGLT ELHPDVFSTAPRLDILHQVAIWQKNFKRISYAKTKTRAEVRGGGRKPWVQKGSGRARHGSIRSPIWRGGGVAHGPRGPTS YYYMLPMKVRVQGLKVALTVKLAQDDLHIVDSLELPTADPQYLIELARYRRWGDSVLLVDLEHEDMPQNVVAATSGLKTF NLVPAVGLNVHSMLKHQTLVLTLPTVAFLEEKLLWHNSRYTPLYPFRLPYCDFP A0A287B9C4 Mitochondrial ribosomal protein L9 Sus scrofa 0.030373449 1 LEVO MAAAACTAAGGGLLRAGLERLLRGGVRALLRPRLEGVTPRSERAFSLSLSRSTVIVERWWKVPLAGEGRKPRLHRRHRVY KLVEDTKHRPKDNLELVLTQSVEELGVRGDLVSVKKSVGRNRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKLQTKAG EATVKFLRRCHLEVGMKNNVKWELNPEIVARHFLRNLGVVVAPHALKLPEEPITQRGEYWCEVTVNGLDTVRVPMSVVNF ERPKTKRYKYWLAQQAAKGDVPTSSQMI I3LR45 Mitochondrial ribosomal protein L10 Sus scrofa 0.029449688999999998 1 LEVO MAAVVAGMLRGGLLPQAGRLPTFQTVRYGSKAVTRHRRVMHFERQKLMAVTEYIPPKPTINPRCLPPPPTPPQEETGLVR LLRREIAAVFRDNRMIAVCQHVALSAEDKLLLRHQLRKHKILMKIFPNQVLKPFLEESKYQNLLPLFVGHNLLLVSEEPK VKEMVRILKGIPFLPLLGGCIDDTILSRQGFINYSKLPSLALVQGELVGGLTFLTAQTYSMLQHQPRQLTALLDQYVKQQ QEGHPAVPASGQPEPPDPVPDP F1RWJ0 Mitochondrial ribosomal protein L11 Sus scrofa 0.020809271 1 LEVO MSKLSRATRALKKPEASGMIRAIVRAGQARPGPPLGPILGQRGVSINQFCKEFNEKTKDIKEGIPLPTKIFVKPDRTFEI KIGQPTVSYFLKAAAGIEKGARHTGKEVAGLVTLKHVYEIARVKAQDDAFALQDVPLSSVVRSIIGSARSLGIRVVKDLS SEELAAFQKERALFLAAQREADLAAQAEAAKK F1RU54 Uncharacterized protein Sus scrofa 0.020633801 1 LEVO MSSFSKAPQQWATFARVWYLLDGKMQPPGKLAAMASVKLQGLHKPVYHQLSDCGDHVVIMNTRHIAFSGNKWEQKVYSSH TGYPGGFRQVTAAQLHQKDPVAIVKLAIYGMLPKNLHRRTMMQRLHLFPDEDIPEDILKNLVEELPQPRKVPRRLDEYTQ EEIEAFPRVWSPPEDYRL K7GP19 Uncharacterized protein Sus scrofa 0.015955631999999997 1 LEVO MAFCTGLRGPFAQVSRALSQRCFSTTGSLSAIQKMTRVRVVDNSALGTTPYHRPPRCIHVYNKTGVGKVGDRILLAIRGQ KKKALIVGHRMPGPRMTPRFDSNNVVLLEDNGNPVGTRIKTPIPSSLRQREGEFSKVLAIAQNFV A0A287BMZ6 Uncharacterized protein Sus scrofa 0.033481723 1 LEVO MGGPVQGGGPRALDLLRALPRVSLANLKPNPGSRKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYLRIPK YGFNEGHSFRHQYQPLSLNRLQYLIDLGRVDPTQPIDLTQLVNGRGVTIQPSKRDYGVQLVEEGADTFKAKVNIEVQMAS ELAIAAIEKNGGVVTTAFYDPRSLEILCKPVPFFLRGQPIPKRMLPPEALVPYYTDAKNRGYLADPARFPEARLELARKY GYVLPDITKDELFKMLSTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYSS F1RSH0 Uncharacterized protein Sus scrofa 0.028680531 1 LEVO MWRLLARACAPLLRAPLSDSWAAPSATAGFKTLLPVPTFEDVAIPEKPKLRFVERVPLVPKVRRERKNLSDIRGPSTEAT EFTEGRFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFALWRVPAPFKPITRKGMGQRMGGGKGAIDHYVTPVKAGRLI VEMGGRCEFKEVQGFLDLVAHKLPFPAKAVSRETLEKMRKDQEERERNNQNPWTFERIATANMLGIRKVLSPYDLTHKGR YWGKFYTPERV F1RI89 Uncharacterized protein Sus scrofa 0.019268178 1 LEVO MRLSVAAAISHGRVFRRLGLGPESRIHLLQNLLTGLVRHERIEASWARVDELRGYAEKLIDYGKLGDTNERAMRMADFWL TEKDLIPKLFQVLAPRFQGQNGGYTRMLQIPNRNEQDRAKMAVIEYKGNCLPPLPLPRRDSNLTLLNQLLQGLRQDQEAS SHTAQTPAV F1RMQ4 Mitochondrial ribosomal protein L18 Sus scrofa 0.020582568 1 LEVO MALRSRFWALLSICRNPGCRAAALSTSCKPATNPETDPVENEAVAPEFTNRNPRNLELLAVARKERGWGTVWPSREFWHR LRVIRTQHHIEALVEHRNGQVVVSASTREWAIKKHLYSTRNVVACESVGRVLAERCLEAGINFMVYHPTPWEAASDSIKR LQHAMTEGGVVLREPRRIYE A0A286ZNK1 MAGNESIUM ION 2.4305e-05 325 MG ZINC ION 6.5409e-05 6 ZN SPERMINE 0.00020234 3 SPM N-FORMYLMETHIONINE 0.000177221 1 FME GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP GUANOSINE-5'-MONOPHOSPHATE 0.000363221 1 5GP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER 0.000522196 1 GNP water 1.8015e-05 5 HOH singleParticle particle 0.017 7.6 ETHANE-PROPANE FEI TITAN KRIOS FLOOD BEAM OTHER FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) 40.0 1 C1 4.2 FSC 0.143 CUT-OFF 12858 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_10779.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.16235815 0.2894498 0.0008609195 0.012041429 1.39 1.39 1.39 0.045 AUTHOR ::::EMDATABANK.org::::EMD-10779:::: RIGID BODY FIT REAL emd_10779_msk_1.map emd_10779_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.07107983 0.14951308 0.00028074312 0.01051298 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-10779:::: emd_10779_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 444.8 444.8 444.8 90.0 90.0 90.0 X Y Z -0.072837666 0.14444956 0.0002724892 0.010361488 1.39 1.39 1.39 AUTHOR ::::EMDATABANK.org::::EMD-10779::::