REL PDBe PDBe PDBe 2004-04-14 2004-04-14 2005-04-14 2011-05-26 Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications. Agrawal RK Agrawal RK Sharma MR Kiel MC Hirokawa G Booth TM Spahn CM Grassucci RA Kaji A Frank J Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications. PROC.NAT.ACAD.SCI.USA 101 8900 8905 2004 15178758 doi:10.1073/pnas.0401904101 1t1m FULLOVERLAP 1t1o FULLOVERLAP 70S-RRF complex of e.coli 70S-RRF complex of e.coli 2 2.5 2.5 sedimentation 70S 2.48 2.48 ribosome-prokaryote: ALL RRF Escherichia coli e.coli bacteria bacteria cytosol 0.02 0.02 1 monomer false singleParticle particle 7.5
52 mM Tris-HCL (pH7.5), 25 mM KCL, 5.5 mM NH4Cl, 11 mM Mg(OAc)2 0.3 mM Beta-ME
quantifoil grids with holy carbon
 ETHANE 30 93 HOMEMADE PLUNGER
Vitrification instrument: two side blotting plunger
Blot for 2 seconds before plunging FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 1.4 4.4 50000.0 49696.0 GATAN LIQUID NITROGEN 93 2002-06-01 KODAK SO-163 FILM ZEISS SCAI 14 76 20 1.2 12. Cryo transfer 0 0
defocus groups
 C1 OTHER 12.0 FSC 0.5 CUT-OFF SPIDER
Final map was calculated from 23 CTF corrected defocus groups
51217
SPIDER: theta 15 degrees, phi 15 degrees
 emd_1077.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 -65 -65 -65 130 130 130 366.6 366.6 366.6 90 90 90 X Y Z -0.00396945 0.00668052 0.000286843 0.000751109 2.82 2.82 2.82 0.0017 Complex of e.coli 70S ribosome and ribosome recycling factor (RRF).
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 RIGID BODY FIT manual
Protocol: Rigid Body. the RRF was fitted as single rigid body
cross correlation coefficient