<?xml version='1.0' encoding='utf-8'?>
<emd emdb_id="EMD-10767" version="3.0.2.4" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_2_4/emdb.xsd">
    <admin>
        <current_status>
            <date>2021-04-14</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-03-15</deposition>
            <header_release>2020-08-05</header_release>
            <map_release>2020-08-05</map_release>
            <update>2021-04-14</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>European Research Council (ERC)</funding_body>
                <code>FP7 GA ERC-2012-387 SyG_318987-ToPAG</code>
                <country>Germany</country>
            </grant_reference>
            <grant_reference>
                <funding_body>German Research Foundation (DFG)</funding_body>
                <code>GSC-1006</code>
                <country>Germany</country>
            </grant_reference>
            <grant_reference>
                <funding_body>German Research Foundation (DFG)</funding_body>
                <code>EXC 2067/1- 390729940</code>
                <country>Germany</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-electron tomogram and segmentation of the cortical ER in yeast, used for testing surface extraction algorithms.</title>
        <authors_list>
            <author>Collado JF</author>
            <author>Salfer M</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Salfer M</author>
                    <author order="2">Collado JF</author>
                    <author order="3">Baumeister W</author>
                    <author order="4">Fernandez-Busnadiego R</author>
                    <author order="5">Martinez-Sanchez A</author>
                    <title>Reliable estimation of membrane curvature for cryo-electron tomography.</title>
                    <journal_abbreviation>Plos Comput.Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>16</volume>
                    <first_page>e1007962</first_page>
                    <last_page>e1007962</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32776920</external_references>
                    <external_references type="DOI">doi:10.1371/journal.pcbi.1007962</external_references>
                    <external_references type="ISSN">1553-7358</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-10767</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Saccharomyces cerevisiae strain ANDY129 deltaScs2 deltaScs22 deltaIst2.</name>
        <supramolecule_list>
            <organelle_or_cellular_component_supramolecule supramolecule_id="1">
                <name>Saccharomyces cerevisiae strain ANDY129 deltaScs2 deltaScs22 deltaIst2.</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                    <organelle>ER, plasma membrane</organelle>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="4932">Saccharomyces cerevisiae</recombinant_organism>
                    <recombinant_strain>ANDY129</recombinant_strain>
                </recombinant_expression>
            </organelle_or_cellular_component_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>6.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage units="kV">30</voltage>
                            <current units="nA">0.03</current>
                            <duration units="s">4000</duration>
                            <temperature units="K">93</temperature>
                            <initial_thickness units="nm">1000</initial_thickness>
                            <final_thickness units="nm">200</final_thickness>
                            <details>The value given for _emd_sectioning_focused_ion_beam.instrument is Quanta 3D Cryo-FIB / SEM. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.1</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">5.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">5.0</nominal_defocus_max>
                    <nominal_magnification>42000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <temperature>
                        <temperature_min units="K">80.0</temperature_min>
                        <temperature_max units="K">90.0</temperature_max>
                    </temperature>
                    <alignment_procedure>
                        <basic />
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Quantum LS</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_exposure_time units="s">1.6</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">1.7</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <algorithm>BACK PROJECTION</algorithm>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <number_images_used>43</number_images_used>
                </final_reconstruction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="675169">
        <file>emd_10767.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>928</col>
            <row>928</row>
            <sec>196</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>98</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>928</x>
            <y>928</y>
            <z>196</z>
        </spacing>
        <cell>
            <a units="Å">12695.04</a>
            <b units="Å">12695.04</b>
            <c units="Å">2681.28</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-204.81978</minimum>
            <maximum>186.58344</maximum>
            <average>4.100616</average>
            <std>18.328278</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">13.68</x>
            <y units="Å">13.68</y>
            <z units="Å">13.68</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-10767::::</label>
        <annotation_details>Raw tomogram.</annotation_details>
    </map>
    <interpretation>
        <segmentation_list>
            <segmentation>
                <file>emd_10767_msk_1.map</file>
            </segmentation>
        </segmentation_list>
        <additional_map_list>
            <additional_map format="CCP4" size_kbytes="68910">
                <file>emd_10767_additional_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>421</col>
                    <row>341</row>
                    <sec>120</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>421</x>
                    <y>341</y>
                    <z>120</z>
                </spacing>
                <cell>
                    <a units="Å">5759.2803</a>
                    <b units="Å">4664.88</b>
                    <c units="Å">1641.6001</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-87.87292</minimum>
                    <maximum>90.43987</maximum>
                    <average>4.1409173</average>
                    <std>13.415353</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">13.68</x>
                    <y units="Å">13.679999</y>
                    <z units="Å">13.680001</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-10767::::</label>
                <annotation_details>Filtered subset of the tomogram using TOMOEED, an anisotropic nonlenear diffusion filter (Fernandez, Lucic 2011).</annotation_details>
            </additional_map>
        </additional_map_list>
    </interpretation>
</emd>