<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" emdb_id="EMD-1076" version="3.0.1.1" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_1/emdb_relaxed.xsd">
   <admin>
      <current_status>
         <code>REL</code>
         <processing_site>PDBe</processing_site>
      </current_status>
      <sites>
         <deposition>PDBe</deposition>
         <last_processing>PDBe</last_processing>
      </sites>
      <key_dates>
         <deposition>2004-03-10</deposition>
         <header_release>2004-04-07</header_release>
         <map_release>2004-04-07</map_release>
         <update>2011-05-26</update>
      </key_dates>
      <title>Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.</title>
      <authors_list>
         <author>Spahn CM</author>
      </authors_list>
      <keywords>
         </keywords>
   </admin>
   <crossreferences>
      <citation_list>
         <primary_citation>
            <journal_citation published="true">
               <author order="1">Spahn CM</author>
               <author order="2">Beckmann R</author>
               <author order="3">Eswar N</author>
               <author order="4">Penczek PA</author>
               <author order="5">Sali A</author>
               <author order="6">Blobel G</author>
               <author order="7">Frank J</author>
               <title>Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.</title>
               <journal>CELL(CAMBRIDGE,MASS.)</journal>
               <volume>107</volume>
               <first_page>373</first_page>
               <last_page>386</last_page>
               <year>2001</year>
               <external_references type="PUBMED">11701127</external_references>
            </journal_citation>
         </primary_citation>
      </citation_list>
   </crossreferences>
   <sample>
      <name>the 80S Ribosome from Saccharomyces cerevisiae</name>
      <supramolecule_list>
         <sample_supramolecule supramolecule_id="1000">
            <name>the 80S Ribosome from Saccharomyces cerevisiae</name>
            <number_unique_components>3</number_unique_components>
            <molecular_weight>
               <theoretical units="MDa">3.2</theoretical>
            </molecular_weight>
         </sample_supramolecule>
         <complex_supramolecule supramolecule_id="1">
            <name>80S ribosome</name>
            <recombinant_exp_flag>false</recombinant_exp_flag>
            <natural_source database="NCBI">
               <organism ncbi="4932">Saccharomyces cerevisiae</organism>
               <synonym_organism>Bakers' yeast</synonym_organism>
            </natural_source>
            <recombinant_expression database="NCBI">
               </recombinant_expression>
            <ribosome-details>ribosome-eukaryote: ALL</ribosome-details>
         </complex_supramolecule>
      </supramolecule_list>
      <macromolecule_list>
         <rna macromolecule_id="1">
            <name>tRNA</name>
            <natural_source database="NCBI">
               <organism ncbi="4932">Saccharomyces cerevisiae</organism>
               <synonym_organism>Baker's yeast</synonym_organism>
            </natural_source>
            <classification>TRANSFER</classification>
            <structure>DOUBLE HELIX</structure>
            <synthetic_flag>false</synthetic_flag>
         </rna>
         <protein_or_peptide macromolecule_id="2">
            <name>Sec61</name>
            <natural_source database="NCBI">
               <organism ncbi="4932">Saccharomyces cerevisiae</organism>
               <synonym_organism>Baker's yeast</synonym_organism>
            </natural_source>
            <recombinant_exp_flag>true</recombinant_exp_flag>
            <recombinant_expression database="NCBI">
               <recombinant_organism ncbi="4932">Saccharomyces cerevisiae</recombinant_organism>
            </recombinant_expression>
            <sequence>
               </sequence>
         </protein_or_peptide>
      </macromolecule_list>
   </sample>
   <structure_determination_list>
      <structure_determination structure_determination_id="1">
         <method>singleParticle</method>
         <aggregation_state>particle</aggregation_state>
         <specimen_preparation_list>
            <single_particle_preparation preparation_id="1">
               <vitrification>
                  <cryogen_name>ETHANE</cryogen_name>
               </vitrification>
            </single_particle_preparation>
         </specimen_preparation_list>
         <microscopy_list>
            <single_particle_microscopy microscopy_id="1">
               <microscope>FEI TECNAI F20</microscope>
               <illumination_mode>FLOOD BEAM</illumination_mode>
               <imaging_mode>BRIGHT FIELD</imaging_mode>
               <electron_source>FIELD EMISSION GUN</electron_source>
               <acceleration_voltage units="kV">200</acceleration_voltage>
               <nominal_cs units="mm">2.0</nominal_cs>
               <nominal_defocus_min units="&#181;m">1.7</nominal_defocus_min>
               <nominal_defocus_max units="&#181;m">6.0</nominal_defocus_max>
               <nominal_magnification>50000.0</nominal_magnification>
               <calibrated_magnification>52000.0</calibrated_magnification>
               <specimen_holder_model>GATAN LIQUID NITROGEN</specimen_holder_model>
               <temperature>
                  <temperature_average units="K">93</temperature_average>
               </temperature>
               <image_recording_list>
                  <image_recording>
                     <film_or_detector_model category="FILM">KODAK SO-163 FILM</film_or_detector_model>
                     <digitization_details>
                        <scanner>ZEISS SCAI</scanner>
                     </digitization_details>
                     <bits_per_pixel>12.</bits_per_pixel>
                  </image_recording>
               </image_recording_list>
               <specimen_holder>cryo-transfer</specimen_holder>
            </single_particle_microscopy>
         </microscopy_list>
         <singleparticle_processing image_processing_id="1">
            <ctf_correction>
               <details>defocus group volumes</details>
            </ctf_correction>
            <final_reconstruction>
               <applied_symmetry>
                  <point_group>C1</point_group>
               </applied_symmetry>
               <algorithm>OTHER</algorithm>
               <resolution res_type="BY AUTHOR" units="&#8491;">15.4</resolution>
               <resolution_method>FSC 0.5 CUT-OFF</resolution_method>
               <software_list>
                  <software>
                     <name>SPIDER</name>
                  </software>
               </software_list>
            </final_reconstruction>
         </singleparticle_processing>
      </structure_determination>
   </structure_determination_list>
   <map format="CCP4" size_kbytes="7631">
      <file>emd_1076.map.gz</file>
      <symmetry>
         <space_group>1</space_group>
      </symmetry>
      <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
      <dimensions>
         <col>125</col>
         <row>125</row>
         <sec>125</sec>
      </dimensions>
      <origin>
         <col>-62</col>
         <row>-62</row>
         <sec>-62</sec>
      </origin>
      <spacing>
         <x>125</x>
         <y>125</y>
         <z>125</z>
      </spacing>
      <cell>
         <a units="&#8491;">366.25</a>
         <b units="&#8491;">366.25</b>
         <c units="&#8491;">366.25</c>
         <alpha units="deg">90</alpha>
         <beta units="deg">90</beta>
         <gamma units="deg">90</gamma>
      </cell>
      <axis_order>
         <fast>X</fast>
         <medium>Y</medium>
         <slow>Z</slow>
      </axis_order>
      <statistics>
         <minimum>-89.955600000000004</minimum>
         <maximum>227.358000000000004</maximum>
         <average>4.7599</average>
         <std>29.0471</std>
      </statistics>
      <pixel_spacing>
         <x units="&#8491;">2.93</x>
         <y units="&#8491;">2.93</y>
         <z units="&#8491;">2.93</z>
      </pixel_spacing>
      <contour_list>
         <contour primary="true">
            <level>78.0</level>
         </contour>
      </contour_list>
      <annotation_details>This is a volume of 80S ribosome</annotation_details>
      <details>::::EMDATABANK.org::::EMD-1076::::</details>
   </map>
</emd>