2021-04-14 REL PDBe PDBe PDBe 2020-03-12 2020-07-29 2020-07-29 2021-04-14 Wellcome Trust 207455/Z/17/Z United Kingdom Medical Research Council (MRC, United Kingdom) MC_UP_1201/16 United Kingdom AP2 in clathrin coats assembled on a membrane containing tyrosine-based cargo peptide Kovtun O Kane Dickson V Kelly BT Owen D Briggs JAG Kovtun O Dickson VK Kelly BT Owen DJ Briggs JAG Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles. Sci Adv US 6 eaba8381 eaba8381 2020 32743075 doi:10.1126/sciadv.aba8381 2375-2548 EMD-10747 other EM volume
AP2 core in physiological buffer
 EMD-10748 other EM volume EMD-10749 other EM volume EMD-10750 associated EM volume EMD-10751 other EM volume EMD-10752 other EM volume EMD-10753 other EM volume EMD-10754 other EM volume Clathrin/AP2 coat assembled on AP2 membrane. Clathrin/AP2 coat assembled on AP2 membrane. 0 1 2 3 4
Clathrin/AP coat was formed on the membrane containing dileucine- and tyrosine-based cargo signal peptides. The AP2 adaptor lacked hinge and appendage regions in its alpha subunit.
 Clathrin 1
Clathrin in the clathrin/AP2 coat formed on the membrane
Sus scrofa brain AP2 1
AP2 in clathrin coat formed on membranes containing dileucine- and tyrosine-based cargo peptides
Homo sapiens Escherichia coli 'BL21-Gold(DE3)pLysS AG' subtomogramAveraging threeDArray 0.7 7.2 10.0 Hepes KOH 120.0 Potassium Acetate 2.0 Magnesium chloride 5.0 2-Mercaptoethanol C-flat-2/2 CARBON HOLEY ETHANE 98 291 LEICA EM GP
The sample was supplemented with 10 nm nanogold fiducials, and 3 ul of the mixture was backside blotted for 3 seconds..
The sample (in vitro budding reaction) contained AP2, clathrin and 400 nm extruded liposomes
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 1.5 1.5 6.5 6.5 81000.0 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 QUANTUM (4k x 4k) COUNTING 3838 3710 1-10 2 0.2 3.2
The images were collected in movie mode at 10 frames per second
 1
The images were low pass filtered according to the cumulative radiation dose.
C1 11.9 FSC 0.143 CUT-OFF TOM AV3 14805 25694 224577 reference-free geometrically defined initial positions on membrane strctures TOM NOVACTF 1.0
CTF correction in novaCTF with by multiplication
 OTHER TOM AV3 emd_10750.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 0 0 0 120 120 120 213.59999 213.59999 213.59999 90.0 90.0 90.0 X Y Z -2.501963 2.8607073 0.0015377516 0.22353551 1.78 1.78 1.78 0.32 AUTHOR Sharpened map of AP2 in clathrin coat formed on membranes containing tyrosine-based cargo peptides 1XA7 FLEXIBLE FIT correlation coefficient REAL emd_10750_msk_1.map emd_10750_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 0 0 0 120 120 120 213.59999 213.59999 213.59999 90.0 90.0 90.0 X Y Z -1.2441205 1.2019272 0.0020032844 0.17569754 1.78 1.78 1.78 AUTHOR emd_10750_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 0 0 0 120 120 120 213.59999 213.59999 213.59999 90.0 90.0 90.0 X Y Z -1.0021917 1.0983585 0.002064072 0.14233294 1.78 1.78 1.78 AUTHOR