REL PDBe PDBe PDBe 2004-03-23 2004-03-29 2004-03-29 2011-05-26 Molecular architecture of the prolate head of bacteriophage T4. Fokine A Chipman PR Leiman PG Mesyanzhinov VV Rao VB Rossmann MG Fokine A Chipman PR Leiman PG Mesyanzhinov VV Rao VB Rossmann MG Molecular architecture of the prolate head of bacteriophage T4. PROC.NAT.ACAD.SCI.USA 101 6003 6008 2004 15071181 doi:10.1073/pnas.0400444101 bacteriophage T4 prolate head bacteriophage T4 prolate head
Sample was the native bacteriophage T4. Only the head part of the phage was reconstructed.
1 194 STEM, 194 MDa is the mass of the DNA filled head. Enterobacteria phage T4 Enterobacteria phage T4 Ecoli BACTERIA(EUBACTERIA) 194 capsid width is 860 A, length is 1195 A Tmid=20; Tend=13 VIRION SPECIES false false phage T4 singleParticle particle 6.2
Distilled water. The concentration of the virus particles was 10E11 particles pre ml.
quantifoil copper grid 2um holes
 ETHANE 90 HOMEMADE PLUNGER
Vitrification instrument: in-house, gravity driven plunger
3.5ul of sample hand blotted approx. 1sec FEI/PHILIPS CM300FEG/T FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.0 1.1 2.7 45000.0 47000.0 GATAN LIQUID NITROGEN 97 live fft at 190,000x KODAK SO-163 FILM ZEISS SCAI 7 55 29 8. na
Each particle
 C5 OTHER 22.5 FSC 0.33 CUT-OFF SPIDER
The reconstruction was performed by imposing 5-fold symmetry averaging. The 5-fold axis is along Z.
5140
The reconstruction was made in SPIDER using the projection matching protocol. The reference projections were calculated in the interval theta: 0-90 degrees, phi: 0-72 degrees.
 emd_1075.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 175 175 240 0 0 0 175 175 240 1043 1043 1430.4 90 90 90 X Y Z -0.157651 0.263553 0.0133426 0.0458542 5.96 5.96 5.96 0.0864 cryoEM reconstruction of the bacteriophage T4 head
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Nothing to fit
emd_2431.tif