2021-04-14
REL
PDBe
PDBe
PDBe
2020-03-11
2020-11-11
2020-11-11
2021-04-14
European Research Council (ERC)
Czech Republic
Leishmania RNA virus capsid with exogenous mRNA
Prochazkova M
Prochazkova M
Fuzik T
Grybchuk D
Falginella FL
Podesvova L
Yurchenko V
Vacha R
Plevka P
Capsid Structure of Leishmania RNA Virus 1.
J.Virol.
US
95
2021
33208443
doi:10.1128/JVI.01957-20
1098-5514
0825
JOVIAM
EMD-10722
other EM volume
Virus-like particle
EMD-10745
associated EM volume
Leishmania RNA virus 1 - LgM5313
Leishmania RNA virus 1 - LgM5313
0
1
Assembled particles mixed with isolated Leishmania mRNA
Leishmania RNA virus 1 - LgM5313
Leishmania guyanensis
MHOM/BR/75/M4147
Escherichia coli BL21(DE3)
capsid
422.0
2
VIRUS-LIKE PARTICLE
OTHER
false
true
singleParticle
particle
15
8.0
300.0
NaCl
sodium chloride
50.0
Tris
1.0
EDTA
5.0
B-me
betamercaptoethanol
Supplemented with isolated mRNA from Leishmania sp.
Quantifoil R2/1
COPPER
300
CARBON
HOLEY
ETHANE
70
295
FEI VITROBOT MARK II
blot force 3
blot time 5
wait time 10.
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
50.0
-0.0005
0.0005
75000.0
FEI TITAN KRIOS AUTOGRID HOLDER
NITROGEN
FEI FALCON III (4k x 4k)
COUNTING
1
8000
0.5
21.0
1
16715
CTFFIND
4
1M1C
Initial model constructed.
1
I
3.76
FSC 0.143 CUT-OFF
RELION
9932
ANGULAR RECONSTITUTION
RELION
2.1
PROJECTION MATCHING
1.8
RELION
3
7000.0
RELION
1M1C
A
RIGID BODY FIT
PDB ID: 6Y83 fits the map
R-factor
RECIPROCAL
174.0