2020-06-17 REL PDBe PDBe PDBe 2020-03-11 2020-06-03 2020-06-03 2020-06-17 European Research Council (ERC) 820102 United Kingdom Microtubule Nucleation by Single Human gamma-TuRC in a Partly Open Asymmetric Conformation Locke J Costa A Consolati T Locke J Roostalu J Chen ZA Gannon J Asthana J Lim WM Martino F Cvetkovic MA Rappsilber J Costa A Surrey T Microtubule Nucleation Properties of Single Human gamma TuRCs Explained by Their Cryo-EM Structure. Dev.Cell US 53 603 617.e8 2020 32433913 doi:10.1016/j.devcel.2020.04.019 1534-5807 EMD-10744 associated EM volume gamma-tubulin ring complex gamma-tubulin ring complex 0 1 Homo sapiens Homo sapiens HeLa-Kyoto cells 2.2 gamma-tubulin ring complex Homo sapiens
Complex consists of 5x native GCP2/GCP with mBFP tag, 5x GCP3, 2x GCP4, 1x GCP5, 1x GCP6 and other factors
Homo sapiens LEVO MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTPEDFLKKYDELKSKNTRNLDPLVYLLSKLTEDKETLQYLQQNAKERAELAAAAVGSSTTSINVPAAASKISMQELEELRKQLGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTDTALPIGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNLDLSIRELVHRILPVAASYSAVTRFIEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRTMDILASLATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLEKWIYRGIIHDPYSEFMVEEHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRECGHDVTCPVAKEIIYTLKERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLAEEELRKPVEDITPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQGGGGGENLYFQGGGGGGSEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNGGGGGGLNDIFEAQKIEWHE singleParticle particle 7.4 Homemade COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE AIR ETHANE 90 298 FEI VITROBOT MARK IV
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 3.5 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS GATAN K2 QUANTUM (4k x 4k) COUNTING 2 2400 9.0 50.0 1 Gctf v.1.18
ab initio
 C1 4.0 FSC 0.143 CUT-OFF RELION 522496 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_10744.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 552.96 552.96 552.96 90.0 90.0 90.0 X Y Z -0.049079012 0.062492684 0.00005164352 0.00091257173 1.08 1.08 1.08 0.05 AUTHOR Cryo-EM structure of human gamma-TuRC