2021-04-28 REL PDBe PDBe PDBe 2020-03-09 2020-04-08 2020-04-08 2021-04-28 Medical Research Council (MRC, United Kingdom) MC_UU_12014/7 United Kingdom Medical Research Council (MRC, United Kingdom) MC_PC_17135 United Kingdom Lumazine Synthase Bhella D Streetley J Clarke M Cowton V Patel A Bammes B Hosogi N Bhella D Cryo-electron microscopy: an introduction to the technique, and considerations when working to establish a national facility. Biophys Rev 11 515 519 2019 31359340 doi:10.1007/s12551-019-00571-w 1867-2450 EMD-10736 associated EM volume Icosahedral complex of Lumazine Synthase Icosahedral complex of Lumazine Synthase 0 Aquifex aeolicus Escherichia coli singleParticle particle 3 7.2 C-flat-1.2/1.3 4C COPPER 400 CARBON HOLEY ARRAY GLOW DISCHARGE ETHANE FEI VITROBOT MARK IV
Purified by immobilised metal affinity chromatography.
 JEOL CRYO ARM 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 200000.0 JEOL CRYOSPECPORTER NITROGEN In-column Omega Filter 20 DIRECT ELECTRON DE-64 (8k x 8k) COUNTING 4096 4096 13.0 1-41 1 1199 10.0 1.66
Images were acquired at 141 FPS with 2 x binning and electron counting. A total of 41 summed frames were written out.
 1 198385
Automated particle picking in Relion
 RELION 3.1b with GCTF
SGD
 I FOURIER SPACE 2.0 FSC 0.143 CUT-OFF RELION 3.1b 16258 PROJECTION MATCHING RELION 3.1b PROJECTION MATCHING RELION 3.1b emd_10736.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 238.8 238.8 238.8 90.0 90.0 90.0 X Y Z -0.05662378 0.0998149 -0.00012479199 0.007047362 0.597 0.597 0.597 0.025 AUTHOR Reconstruction of Lumazine Synthase, sharpened and filtered at 2 angstroms emd_10736_msk_1.map emd_10736_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 238.8 238.8 238.8 90.0 90.0 90.0 X Y Z -0.017551756 0.03793932 -0.00012778175 0.0031533912 0.597 0.597 0.597 AUTHOR Half map 2 emd_10736_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 238.8 238.8 238.8 90.0 90.0 90.0 X Y Z -0.019451553 0.038764212 -0.00012175626 0.0032257873 0.597 0.597 0.597 AUTHOR Half map 1