2024-11-13 REL PDBe PDBe PDBe 2020-03-06 2020-08-26 2020-08-26 2024-11-13 European Research Council (ERC) 309657 France Structure of full-length CD20 in complex with Ofatumumab Fab Kumar A Fronzes R Reyes N Cancer immunotherapy, Therapeutic antibody, MEMBRANE PROTEIN Kumar A Planchais C Fronzes R Mouquet H Reyes N Binding mechanisms of therapeutic antibodies to human CD20. Science US 369 793 799 2020 32792392 doi:10.1126/science.abb8008 1095-9203 0038 SCIEAS EMD-10733 other EM volume
6Y97 same protein complex but focused refined
 EMD-10732 other EM volume
CD20 in complex with Ofatumumab Fab
 EMD-10731 other EM volume
CD20 in complex with Rituximab
 6y9a FULLOVERLAP Full-length human antigen CD20 in complex with Obinutuzumab Full-length human antigen CD20 in complex with Obinutuzumab 0 1 2 3 Full-length human CD20 1 1 Homo sapiens Obinutuzumab Fab 1 2 3 Homo sapiens B-lymphocyte antigen CD20 Homo sapiens B-Lymphocyte 0.018735373 2 Homo sapiens LEVO FMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSL FAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQ ELVIAGIVE P11836 Obinutuzumab Fab heavy chain Homo sapiens 0.023407259999999996 1 Homo sapiens LEVO VQLVQSGAEVKKPGSSVKVSCKASGYAFSYSWINWVRQAPGQGLEWMGRIFPGDGDTDYNGKFKGRVTITADKSTSTAYM ELSSLRSEDTAVYYCARNVFDGYWLVYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWN SGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK Obinutuzumab Fab Light chain Homo sapiens 0.023964822 1 Homo sapiens LEVO DIVMTQTPLSLPVTPGEPASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLVSGVPDRFSGSGSGTDFTLKI SRVEAEDVGVYYCAQNLELPYTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQ SGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC singleParticle particle 1.2 7.4 50.0 HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 100.0 NaCl Sodium Chloride 0.0084 GDN GDN101 0.00168 CHS Cholesterol hemisuccinate Quantifoil R1.2/1.3 GOLD 300 GLOW DISCHARGE AIR ETHANE 100 277.15 FEI VITROBOT MARK IV
Grids were blot for 3.5 Sec..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.8 2.0 NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 1-40 1 10545 42.84 1 1316678 3PP4 3 C1 4.2 FSC 0.143 CUT-OFF cryoSPARC 2.14.2 63547 MAXIMUM LIKELIHOOD cryoSPARC 2.14.2 MAXIMUM LIKELIHOOD cryoSPARC 2.14.2 4 cryoSPARC 2.14.2 emd_10734.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 350 350 350 0 0 0 350 350 350 284.9 284.9 284.9 90.0 90.0 90.0 X Y Z -0.93773156 1.336014 0.0015358375 0.040006608 0.814 0.814 0.814 0.22 AUTHOR Full-length human CD20 in complex with Obinutuzumab 3PP4 H 2-220 PDB experimental model 3PP4 L 1-219 PDB experimental model 6Y90 45-213 PDB experimental model RIGID BODY FIT REAL