2024-10-16 REL PDBe PDBe PDBe 2020-03-03 2020-11-11 2020-11-11 2024-10-16 European Research Council (ERC) Czech Republic Capsid structure of Leishmania RNA virus 1 Prochazkova M Fuzik T virus-like particle, capsid structure, VIRUS LIKE PARTICLE Prochazkova M Fuzik T Grybchuk D Falginella FL Podesvova L Yurchenko V Vacha R Plevka P Capsid Structure of Leishmania RNA Virus 1. J.Virol. US 95 2021 33208443 doi:10.1128/JVI.01957-20 1098-5514 0825 JOVIAM 6y83 FULLOVERLAP Leishmania RNA virus 1 - 4 Leishmania RNA virus 1 - LgM5313 0 1 Leishmania RNA virus 1 - LgM5313 Leishmania guyanensis MHOM/BR/75/M4147 capsid 422.0 2 VIRUS-LIKE PARTICLE OTHER false true Capsid protein Leishmania RNA virus 1 - 4 0.08753692999999999
Subunit A model for residues 15-204, 210-520, 541-635. Subunit B model for residues 19-290, 300-517, 541-576, 583-642.,Subunit A model for residues 15-204, 210-520, 541-635. Subunit B model for residues 19-290, 300-517, 541-576, 583-642.
2 Escherichia coli BL21(DE3) LEVO MADIPNSDKIACGRKPMFCEIIKLANRKRLIFNTTDERVYDARLNYCSTADSTVQADCHIYWRLKLRRTDAVFEEYTGQG YSLDTAAYPQQYTDIIRGYYSKHVSSSLAANTQHFVNVLAMLRHAGACIAHYCMTGKIDFDILSKKKHKNKEVVTLSNAD SLSFLPHSALYLPSPLRASDPEIFNMLYLLGCACDASIAMDNISNTSGAAKYSMPHYNPLQLSHALHVTIFYMLSLMDSC GYGDDAVLALTSGLHSVTTVIAHSDEGGITRDALRELSYTQPYGTMPVPIAGYFQHINVLFTTQPAWDQFAGIWDYVILA TAALVHLSDPGMTVNDVTYPTTLTTKVATVDGRNSDLAAQMMHSATRFCDIFVENLSTFWGVVANPDGNASQALLHAFNI VACAVEPNRHLEMNVMAPWYWVESSALFCDYAPFRSPISSAGYGPQCVYGARLVLAATNSLEFTGEAGDYSAYRFEWTTM RHNPLFNILNKRVGDGLANVDFRLRPFNEWLLEGQPSRRSCNSAGHGTPTATCSHKTPNHDTLDEYIWGSTSCDLFHPAE LTSYTAVCVRFRNYLSGADGDVRILNTPTREVIEGNVVTRCDGIRCLDSNKRIQHVPEVARRYCMMARYLAQARTFGALT IGDDIIRGFDKVEKIVKMHKSNNRLDQMPLIDVTGLCQPMIETSTVRASTTTRIDPNKLAAATARVELPLAPRCTSSLIP SSDTVPEPEPQVGEPGDNGGCAPDLGTGGGSGIEGRGSMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH L7XUU7 singleParticle particle 15 8.0 300.0 NaCl sodium chloride 50.0 Tris 1.0 EDTA 5.0 B-me betamercaptoethanol Quantifoil R2/1 COPPER 300 CARBON HOLEY GLOW DISCHARGE OTHER ETHANE 70 295 FEI VITROBOT MARK II
blot force 3 blot time 5 wait time 10.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 -0.0005 0.0005 75000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) COUNTING 1 12000 0.5 21.0 1 28000 1M1C
Initial model constructed.
 1 I 3.65 FSC 0.143 CUT-OFF RELION 16901 ANGULAR RECONSTITUTION RELION 2.1 PROJECTION MATCHING 1.8 RELION 3 20000.0 RELION emd_10722.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 546 546 546 -273 -273 -273 546 546 546 580.398 580.398 580.398 90.0 90.0 90.0 X Y Z -0.0893611 0.18737605 0.0021453528 0.011328808 1.063 1.063 1.063 0.05 AUTHOR Map rotated to crystallographic orientation with origin in 000. 1M1C A PDB experimental model RIGID BODY FIT R-factor RECIPROCAL 174.0 emd_10722_msk_1.map emd_10722_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 546 546 546 0 0 0 546 546 546 580.398 580.398 580.398 90.0 90.0 90.0 X Y Z -0.09581354 0.19190128 0.002145352 0.011907249 1.063 1.063 1.063 AUTHOR Map in original position, matches mask.