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    <admin>
        <current_status>
            <date>2020-07-22</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-02-28</deposition>
            <header_release>2020-07-22</header_release>
            <map_release>2020-07-22</map_release>
            <update>2020-07-22</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Research Foundation (NRF, Korea)</funding_body>
                <code>NRF-2015K1A1A2033054</code>
                <country>Korea, Republic Of</country>
            </grant_reference>
            <grant_reference>
                <funding_body>European Research Council (ERC)</funding_body>
                <country>Switzerland</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Swiss National Science Foundation</funding_body>
                <country>Switzerland</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Cancer Research UK</funding_body>
                <country>United Kingdom</country>
            </grant_reference>
            <grant_reference>
                <funding_body>The Francis Crick Institute</funding_body>
                <country>United Kingdom</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Wellcome Trust</funding_body>
                <country>United Kingdom</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Medical Research Council (MRC, United Kingdom)</funding_body>
                <country>United Kingdom</country>
            </grant_reference>
        </grant_support>
        <title>Apoferritin from horse spleen, prepared using trEM workflow.</title>
        <authors_list>
            <author>Maeots ME</author>
            <author>Lee B</author>
            <author>Nans A</author>
            <author>Jeong SG</author>
            <author>Esfahani MNM</author>
            <author>Smith D</author>
            <author>Lee CS</author>
            <author>Lee SS</author>
            <author>Peter M</author>
            <author>Enchev RI</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Maeots ME</author>
                    <author order="2">Lee B</author>
                    <author order="3">Nans A</author>
                    <author order="4">Jeong SG</author>
                    <author order="5">Esfahani MMN</author>
                    <author order="6">Ding S</author>
                    <author order="7">Smith DJ</author>
                    <author order="8">Lee CS</author>
                    <author order="9">Lee SS</author>
                    <author order="10">Peter M</author>
                    <author order="11">Enchev RI</author>
                    <title>Modular microfluidics enables kinetic insight from time-resolved cryo-EM.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>11</volume>
                    <first_page>3465</first_page>
                    <last_page>3465</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32651368</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-020-17230-4</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-10712</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Apoferritin from horse spleen</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Apoferritin from horse spleen</name>
                <parent>0</parent>
                <details>commercial apoferritin from Sigma Aldrich</details>
                <natural_source database="NCBI">
                    <organism ncbi="9796">Equus caballus</organism>
                    <organ>spleen</organ>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.443</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">2</concentration>
                    <buffer>
                        <ph>7.4</ph>
                        <component>
                            <name>PBSA</name>
                        </component>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <atmosphere>AIR</atmosphere>
                        </pretreatment>
                        <details>50mA</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_temperature units="K">294</chamber_temperature>
                        <instrument>HOMEMADE PLUNGER</instrument>
                        <details>Plunging speed 1.1m/s, ambient conditions.. </details>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON III (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">33.6</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>O</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">2.77</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>9804</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_10712.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>250</col>
            <row>250</row>
            <sec>250</sec>
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            <col>0</col>
            <row>0</row>
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        <spacing>
            <x>250</x>
            <y>250</y>
            <z>250</z>
        </spacing>
        <cell>
            <a units="Å">211.25</a>
            <b units="Å">211.25</b>
            <c units="Å">211.25</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>Z</fast>
            <medium>Y</medium>
            <slow>X</slow>
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        <statistics>
            <minimum>-0.13326071</minimum>
            <maximum>0.20716992</maximum>
            <average>0.00000000000</average>
            <std>0.012729209</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">0.845</x>
            <y units="Å">0.845</y>
            <z units="Å">0.845</z>
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            <contour primary="true">
                <level>0.0576</level>
                <source>AUTHOR</source>
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        <label>::::EMDATABANK.org::::EMD-10712::::</label>
        <annotation_details>Apoferritin from equine spleen, prepared using trEM workflow</annotation_details>
    </map>
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