2024-05-22 REL PDBe PDBe PDBe 2020-02-26 2020-04-08 2020-04-08 2024-05-22 Leibniz Association K27/2017 Germany German Research Foundation (DFG) INST 152/777-1 FUGG Germany Cryo-EM structure of a Phenuiviridae L protein Vogel D Thorkelsson SR Bunyavirus, Phenuiviridae, L protein, viral polymerase, cap-snatching, VIRAL PROTEIN Vogel D Thorkelsson SR Quemin ERJ Meier K Kouba T Gogrefe N Busch C Reindl S Gunther S Cusack S Grunewald K Rosenthal M Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein. Nucleic Acids Res. UK 48 5749 5765 2020 32313945 doi:10.1093/nar/gkaa253 1362-4962 0389 NARHAD Afonine PV Poon BK Read RJ Sobolev OV Terwilliger TC Urzhumtsev A Adams PD Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Crystallogr D Struct Biol 74 531 544 2018 29872004 doi:10.1107/S2059798318006551 2059-7983 Scheres SH RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. US 180 519 530 2012 23000701 doi:10.1016/j.jsb.2012.09.006 1095-8657 0803 JSBIEM 6y6k FULLOVERLAP SASBDB SASDHQ8 associated SAS data Severe Fever with Thrombocytopenia Syndrome Virus L Protein Severe Fever with Thrombocytopenia Syndrome Virus L Protein 0 1 SFTS virus AH12 0.238 RNA-dependent RNA polymerase SFTS virus AH12 0.23574253099999998 1 Escherichia coli LEVO MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIGAVPDSASQLGSSINAGLITIQLSEAYKINH DFTFSGLSKTTDRRLSEVFPITHDGSDGMTPDVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYRDPISRRVDIM ENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANEIYTKARSMDADIELQKSEEELEAISRALSFFSLFEPNIER VEGTFPNSEIKMLEQFLSTPADVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEINRCEAIDKTQDLLAGLHARSN KQTSLNRGTVKLPPWLPKPSSESIDIKTDSGFGSLMDHGAYGELWAKCLLDVSLGNVEGVVSDPAKELDIAISDDPEKDT PKEAKITYRRFKPALSSSARQEFSLQGVEGKKWKRMAANQKKEKESHETLSPFLDVEDIGDFLTFNNLLTDSRYGDESIQ RAVSILLEKASAMQDTELTHALNDSFKRNLSSNVVQWSLWVSCLAQELASALKQHCRAGEFIIKKLKFWPIYVIIKPTKS SSHIFYSLGIRKADVTRRLTGRVFSDTIDAGEWELTEFKSLKTCKLTNLVNLPCTMLNSIAFWREKLGVAPWLVRKPCSE LREQVGLTFLISLEDKSKTEEIITLTRYTQMEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHLDCMVRIASQPFSL IPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKNKEESTELNALGEMYKKIVEMEEDKPSSPEFLGWGDTDSPKKHE FSRSFLRAACSSLEREIAQRHGRQWKQNLEERVLREIGTKNILDLASMKATSNFSKDWELYSEVQTKEYHRSKLLEKMAT LIEKGVMWYIDAVGQAWKAVLDDGCMRICLFKKNQHGGLREIYVMDANARLVQFGVETMARCVCELSPHETVANPRLKNS IIENHGLKSARSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMPAKFHRFIWAAISMFRRKKMMVDLRFLAHLSSKS ESRSSDPFREAMTDAFHGNRDVSWMDKGRTYIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGYMGESISGVVD VIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLNPLFGIYSSEKSTVNTVYCVEYNSEFHFHRHLVRPTLRWIA ASHQISETEALASRQEDYSNLLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGTFMGMLISDPDPALGFFLMDN PAFAGGAGFRFNLWRACKTTDLGRKYAYYFNEIQGKTKGDEDYRALDATSGGTLSHSVMVYWGDRKKYQALLNRMGLPED WVEQIDENPGVLYRRAANKKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHCFRFSSSIHGRGSTQKASLIK LLMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLLEEVEHLTGKFVVRERNIVRSRIDLFQEPVDLRCKAEDLVSEV WFGLKRTKLGPRLLKEEWDKLRASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKSRSVRLLGAPVKKSGGVTTISQV VRMNFFPGFSLEAEKSLDNQERLESISILKHVLFMVLNGPYTEEYKLEMIIEAFSTLVIPQPSEVIRKSRTMTLCLLSNY LSSRGGSILDQIERAQSGTLGGFSKPQKTFVRPGGGVGYKGKGVWTGVMEDTHVQILIDGDGTSNWLEEIRLSSDARLYD VIESIRRLCDDLGINNRVASAYRGHCMVRLSGFKIKPASRTDGCPVRIMERGFRIRELQNPDEVKMRVRGDILNLSVTIQ EGRVMNILSYRPRDTDISESAAAYLWSNRDLFSFGKKEPSCSWICLKTLDNWAWSHASVLLANDRKTQGIDNRAMGNIFR DCLEGSLRKQGLMRSKLTEMVEKNVVPLTTQELVDILEEDIDFSDVIAVELSEGSLDIESIFDGAPILWSAEVEEFGEGV VAVSYSSKYYHLTLMDQAAITMCAIMGKEGCRGLLTEKRCMAAIREQVRPFLIFLQIPEDSISWVSDQFCDSRGLDEEST IMWG F1BV96 MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 0.15 7.0 20.0 C8H18N2O4S HEPES 500.0 NaCl sodium chloride 20.0 MgCl magnesium chloride Quantifoil R2/1 PLASMA CLEANING
Harrick Plasma cleaner
 ETHANE-PROPANE 100 277.15 FEI VITROBOT MARK IV
Blot for 2s using blotting force -10.
TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.5 3.0 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 (6k x 4k) 1 2626 3.0 59.45 1 780000
Particles were picked with Warp's pretrained agent BoxNet2Mask_20180918
An ab initio model generated by cisTEM from a screening dataset.
 1 C1 FOURIER SPACE 3.78 FSC 0.143 CUT-OFF RELION 3.0 220000 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 6 RELION 3.0 emd_10706.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 174.0 174.0 174.0 90.0 90.0 90.0 X Y Z -0.20976333 0.35944355 0.0008863566 0.013343462 0.87 0.87 0.87 0.05 AUTHOR AB INITIO MODEL REAL 225.217000000000013