2024-11-13 REL PDBe PDBe PDBe 2020-02-25 2020-03-11 2020-03-11 2024-11-13 European Commission 115766 United Kingdom Structure of Human Potassium Chloride Transporter KCC3 in NaCl Chi G Man H Dimer, transporter, potassium chloride, KCC3, SLC12A6, membrane protein, APC, Structural Genomics, Structural Genomics Consortium, SGC, PSI-2, Protein Structure Initiative Chi G Man H Ebenhoch R Reggiano G Pike ACW Wang D McKinley G Mukhopadhyay SMM Chalk R Moreau C Snee M Bohstedt T Singh NK Abrusci P Arrowsmith CH Bountra C Edwards AM Marsden BD Burgess-Brown NA DiMaio F Duerr KL Structural Genomics Consortium (SGC) Structure of Human Potassium Chloride Transporter KCC3 in NaCl To be published 0353 6y5r FULLOVERLAP Homodimeric complex of human potassium chloride transporter Homodimeric complex of human potassium chloride transporter 0 1 Homo sapiens 0.246 Solute carrier family 12 member 6 Homo sapiens 0.12227334399999999
Sugar molecules from glycosylation post-translational modifications (NAG, BMA) present.
2 Homo sapiens LEVO MPHFTVTKVEDPEEGAAASISQEPSLADIKARIQDSDEPDLSQNDITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDK NLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWV VGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIF LVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPH FPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNY LPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVY LSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG HSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSIC LALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHV KHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGM KHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLK QHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERDLMMEQRSQMLRHMRLSKTER DREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMDWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLN EVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS Q9UHW9 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 2 NAG singleParticle particle 6.5 7.5 Quantifoil R1.2/1.3 GOLD 300 CARBON HOLEY GLOW DISCHARGE OTHER ETHANE 100 297 FEI VITROBOT MARK IV
2 second blotting time, 40 second waiting time, -15 blotting force.
This sample was monodisperse.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 -0.8 -2.3000000000000003 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 70.0 70.0 GATAN K3 (6k x 4k) 1 16472 40.0 1
map was built ab initio using Relion 3.0.8's initial model program
 C1 3.76 FSC 0.5 CUT-OFF RELION 3.0.8 920574 RANDOM ASSIGNMENT RELION 3.0.8 MAXIMUM LIKELIHOOD RELION 3.0.8 RELION 3.0.8 emd_10703.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 286.44 286.44 286.44 90.0 90.0 90.0 X Y Z -0.020708172 0.042289127 0.00006021436 0.0012882311 0.651 0.651 0.651 0.01 AUTHOR emd_10703_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 286.44 286.44 286.44 90.0 90.0 90.0 X Y Z -0.023583762 0.04028616 0.000060948514 0.0014969269 0.651 0.651 0.651 AUTHOR emd_10703_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 286.44 286.44 286.44 90.0 90.0 90.0 X Y Z -0.023195619 0.044292096 0.0000594802 0.0014961933 0.651 0.651 0.651 AUTHOR