<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" emdb_id="EMD-1066" version="3.0.1.1" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_1/emdb_relaxed.xsd">
   <admin>
      <current_status>
         <code>REL</code>
         <processing_site>PDBe</processing_site>
      </current_status>
      <sites>
         <deposition>PDBe</deposition>
         <last_processing>PDBe</last_processing>
      </sites>
      <key_dates>
         <deposition>2003-12-23</deposition>
         <header_release>2003-12-23</header_release>
         <map_release>2004-12-23</map_release>
         <update>2013-12-11</update>
      </key_dates>
      <title>Three-dimensional structure of a pre-catalytic human spliceosomal complex B.</title>
      <authors_list>
         <author>Boehringer D</author>
         <author>Makarov EM</author>
         <author>Sander B</author>
         <author>Makarova OV</author>
         <author>Kastner B</author>
         <author>Luehrmann R</author>
         <author>Stark H</author>
      </authors_list>
      <keywords>
         </keywords>
   </admin>
   <crossreferences>
      <citation_list>
         <primary_citation>
            <journal_citation published="true">
               <author order="1">Boehringer D</author>
               <author order="2">Makarov EM</author>
               <author order="3">Sander B</author>
               <author order="4">Makarova OV</author>
               <author order="5">Kastner B</author>
               <author order="6">Luhrmann R</author>
               <author order="7">Stark H</author>
               <title>Three-dimensional structure of a pre-catalytic human spliceosomal complex B.</title>
               <journal>NAT.STRUCT.MOL.BIOL.</journal>
               <volume>11</volume>
               <first_page>463</first_page>
               <last_page>468</last_page>
               <year>2004</year>
               <external_references type="PUBMED">15098019</external_references>
               <external_references type="DOI">doi:10.1038/nsmb761</external_references>
            </journal_citation>
         </primary_citation>
      </citation_list>
   </crossreferences>
   <sample>
      <name>HeLa human pre-catalytic Spliceosome, B delta U1</name>
      <supramolecule_list>
         <sample_supramolecule supramolecule_id="1000">
            <name>HeLa human pre-catalytic Spliceosome, B delta U1</name>
            <number_unique_components>1</number_unique_components>
         </sample_supramolecule>
         <organelle_or_cellular_component_supramolecule supramolecule_id="1">
            <name synonym="ribo nucleo protein complex">spliceosome</name>
            <recombinant_exp_flag>false</recombinant_exp_flag>
            <natural_source database="NCBI">
               <organism ncbi="9606">Homo sapiens</organism>
               <synonym_organism>Human</synonym_organism>
               <cell>HeLa</cell>
            </natural_source>
            <recombinant_expression database="NCBI">
               </recombinant_expression>
         </organelle_or_cellular_component_supramolecule>
      </supramolecule_list>
   </sample>
   <structure_determination_list>
      <structure_determination structure_determination_id="1">
         <method>singleParticle</method>
         <aggregation_state>particle</aggregation_state>
         <specimen_preparation_list>
            <single_particle_preparation preparation_id="1">
               <vitrification>
                  <cryogen_name>ETHANE</cryogen_name>
               </vitrification>
            </single_particle_preparation>
         </specimen_preparation_list>
         <microscopy_list>
            <single_particle_microscopy microscopy_id="1">
               <microscope>FEI/PHILIPS CM200FEG</microscope>
               <illumination_mode>FLOOD BEAM</illumination_mode>
               <imaging_mode>BRIGHT FIELD</imaging_mode>
               <electron_source>FIELD EMISSION GUN</electron_source>
               <acceleration_voltage units="kV">160</acceleration_voltage>
               <specimen_holder_model>GATAN LIQUID NITROGEN</specimen_holder_model>
               <specimen_holder>Eucentric</specimen_holder>
            </single_particle_microscopy>
         </microscopy_list>
         <singleparticle_processing image_processing_id="1">
            <final_reconstruction>
               <applied_symmetry>
                  <point_group>C1</point_group>
               </applied_symmetry>
               <resolution res_type="BY AUTHOR" units="&#8491;">40.0</resolution>
               <resolution_method>OTHER</resolution_method>
            </final_reconstruction>
         </singleparticle_processing>
      </structure_determination>
   </structure_determination_list>
   <map format="CCP4" size_kbytes="1025">
      <file>emd_1066.map.gz</file>
      <symmetry>
         <space_group>1</space_group>
      </symmetry>
      <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
      <dimensions>
         <col>64</col>
         <row>64</row>
         <sec>64</sec>
      </dimensions>
      <origin>
         <col>0</col>
         <row>0</row>
         <sec>0</sec>
      </origin>
      <spacing>
         <x>64</x>
         <y>64</y>
         <z>64</z>
      </spacing>
      <cell>
         <a units="&#8491;">554.24</a>
         <b units="&#8491;">554.24</b>
         <c units="&#8491;">554.24</c>
         <alpha units="deg">90</alpha>
         <beta units="deg">90</beta>
         <gamma units="deg">90</gamma>
      </cell>
      <axis_order>
         <fast>X</fast>
         <medium>Y</medium>
         <slow>Z</slow>
      </axis_order>
      <statistics>
         <minimum>-0.720368</minimum>
         <maximum>0.958293</maximum>
         <average>0.000000000029852</average>
         <std>0.0917209</std>
      </statistics>
      <pixel_spacing>
         <x units="&#8491;">8.66</x>
         <y units="&#8491;">8.66</y>
         <z units="&#8491;">8.66</z>
      </pixel_spacing>
      <contour_list>
         <contour primary="true">
            <level>0.138</level>
         </contour>
      </contour_list>
      <annotation_details>3D map of the spliceosome (B delta U1)</annotation_details>
      <details>::::EMDATABANK.org::::EMD-1066::::</details>
   </map>
</emd>