2025-07-02 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name DATABASE_REFERENCES citation citation_author _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID DATABASE_REFERENCES chem_comp_atom chem_comp_bond database_2 _database_2.pdbx_DOI _database_2.pdbx_database_accession STRUCTURE_SUMMARY em_admin em_software pdbx_entry_details _em_admin.last_update _em_software.name PDBe PDBe 2020-01-30 2020-02-12 2020-02-12 2025-07-02 Medical Research Council (MRC, United Kingdom) MC_UP_A025_1013 United Kingdom Medical Research Council (MRC, United Kingdom) MC_U105184291 United Kingdom Innovative Medicines Initiative 116060 United Kingdom Japan Agency for Medical Research and Development (AMED) JP18ek0109391 Japan Japan Agency for Medical Research and Development (AMED) JP18dm020719 Japan National Institutes of Health/National Institute on Aging (NIH/NIA) P30AG010133 United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) U01NS110437 United States Multiple system atrophy Type II-1 alpha-synuclein filament Schweighauser M Shi Y multiple system atrophy, alpha-synuclein filament, PROTEIN FIBRIL Schweighauser M Shi Y Tarutani A Kametani F Murzin AG Ghetti B Matsubara T Tomita T Ando T Hasegawa K Murayama S Yoshida M Hasegawa M Scheres SHW Goedert M Structures of alpha-synuclein filaments from multiple system atrophy. Nature UK 585 464 469 2020 32461689 doi:10.1038/s41586-020-2317-6 1476-4687 0006 NATUAS 6xyp FULLOVERLAP Sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain Sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain 0 1 Homo sapiens Alpha-synuclein Homo sapiens 0.014476108 10 LEVO MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA P37840 helical filament 7.4 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) COUNTING 47.5 1 4.72 -1.41 C1 3.29 FSC 0.143 CUT-OFF
Helical reconstruction in RELION3. The initial model was reconstructed from reference-free 2-D class averages and the resolution of the cryo-EM map was good enough to do de-novo modelling.
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