REL PDBe PDBe PDBe 2003-12-19 2003-12-22 2004-12-22 2011-05-26 Visualization of release factor 3 on the ribosome during termination of protein synthesis. Klaholz BP Klaholz BP Myasnikov AG Van Heel M Visualization of release factor 3 on the ribosome during termination of protein synthesis. NATURE 427 862 865 2004 14985767 doi:10.1038/nature02332 70S - RF3 complex E.coli 70S - RF3 complex E.coli
two functional states within a single sample of homogeneous composition, separated by image processing techniques, state 2
monomer 4 2.5 70S ribosome-prokaryote: ALL messenger RNA Escherichia coli Escherichia coli GGGCCCUUGUUAACAAUUAAGGAGGUAUACUAUGUUUACGAUUUAAUUGCAGAAAAAAAAAAAAAAAAAAAAA OTHER SINGLE STRANDED true isoleucin transfer RNA Escherichia coli Escherichia coli TRANSFER SINGLE STRANDED false release factor 3 Escherichia coli Escherichia coli Escherichia coli
PrfC gene
monomer true Escherichia coli MKH13 pOSEX3 IPR004548 singleParticle particle 0.15 7.5
polymix buffer
 NEGATIVE
no staining, cryo-EM with holey carbon grids
300 mesh Cu/Rh
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: home-made cryo-plunger
Blot for 2 seconds before plunging FEI/PHILIPS CM200FEG FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.1 1.0 2.8 50000.0 48000.0 GATAN LIQUID NITROGEN lens astigmatism was corrected at 60,000 times magnification 2001-12-19 KODAK SO-163 FILM OTHER 3 44 10
IMAGE SCIENCE PATCHWORK DENSITOMETER
12. Side entry liquid nitrogen-cooled cryo specimen holder
individual particles
 C1 OTHER 17.5 FSC 0.143 CUT-OFF IMAGIC
exact filtered back-projection
10011
beta gamma
 246 emd_1065.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 -60 -60 -60 120 120 120 290.28 290.28 290.28 90 90 90 X Y Z -65.829700000000003 84.578000000000003 -0.13018 9.802809999999999 2.419 2.419 2.419 16.899999999999999 Cryo-EM map of the E.coli 70S/RF3 complex, state 2
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