2023-11-29 REL PDBe PDBe PDBe 2020-01-20 2020-02-19 2020-03-11 2023-11-29 Wellcome Trust WT100237 United Kingdom Cryo EM map of BS3 crosslinked CoREST complex (LSD1:RCOR1:HDAC1) - open form Song Y Fairall L Ragan TJ Savva CG Morone N Schwabe JWR CoREST, RCOR1, HDAC1, LSD1, GENE REGULATION Song Y Dagil L Fairall L Robertson N Wu M Ragan TJ Savva CG Saleh A Morone N Kunze MBA Jamieson AG Cole PA Hansen DF Schwabe JWR Mechanism of Crosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoREST Complex. Cell Rep US 30 2699 2711.e8 2020 32101746 doi:10.1016/j.celrep.2020.01.091 2211-1247 SASBDB SASDH45 associated SAS data Ternary complex of RCOR1 with the demethylase LSD1 and the deacetylase HDAC1 Ternary complex of RCOR1 with the demethylase LSD1 and the deacetylase HDAC1 0 Homo sapiens 0.21 singleParticle particle 0.03 7.5 25.0 HEPES HEPES 50.0 KCl potassium chloride ETHANE 100 277 FEI VITROBOT MARK IV
30 sec waiting time before blotting. Blot time 4 sec, blot force 10..
Sample was crosslinked with 2mM BS3 at room temperature for 5 minutes
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.5 0.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 80.0 GIF Bioquantum 20 GATAN K2 QUANTUM (4k x 4k) COUNTING 3838 3710 1-36 1 1885 14.0 32.0 1 164247
Particles picked using crYOLO
 Relion 3.0 Initial Model C1 21.5 FSC 0.143 CUT-OFF RELION 3 6825 PROJECTION MATCHING RELION 3 PROJECTION MATCHING RELION 3 4 RELION 3 emd_10629.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 280.0 280.0 280.0 90.0 90.0 90.0 X Y Z -0.007937333 0.029882746 0.00017250027 0.0018968353 1.4 1.4 1.4 0.006 AUTHOR 1BKX PDB experimental model 2VID PDB experimental model RIGID BODY FIT emd_10629_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 280.0 280.0 280.0 90.0 90.0 90.0 X Y Z -0.007825799 0.028280599 0.00019237425 0.0018385621 1.4 1.4 1.4 AUTHOR emd_10629_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 280.0 280.0 280.0 90.0 90.0 90.0 X Y Z -0.007465669 0.02921365 0.0001924834 0.0018404603 1.4 1.4 1.4 AUTHOR