2020-03-11 REL PDBe PDBe PDBe 2020-01-20 2020-02-19 2020-03-11 2020-03-11 Wellcome Trust WT100237 United Kingdom Song Y Fairall L Ragan TJ Savva CG Saleh A Morone N Schwabe JWR Song Y Dagil L Fairall L Robertson N Wu M Ragan TJ Savva CG Saleh A Morone N Kunze MBA Jamieson AG Cole PA Hansen DF Schwabe JWR Cell Rep US 30 2699 2711.e8 2020 32101746 doi:10.1016/j.celrep.2020.01.091 2211-1247 EMD-10626 associated EM volume Ternary complex of RCOR1 with the demethylase LSD1 and the deacetylase HDAC1 Ternary complex of RCOR1 with the demethylase LSD1 and the deacetylase HDAC1 0 Homo sapiens Homo sapiens HEK293F pcDNA3 0.21 singleParticle particle 0.01 7.5 25.0 Tris Tris 50.0 KCl potassium chloride NEGATIVE Uranyl Acetate

5 ul of 0.01 mg/ml of the CoREST ternary complex was applied onto the grid and the excess liquid was blotted after 1 minute incubation. 2% uranyl acetate was used to stain the sample.

C-flat COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE

10 mA

Sample was crosslinked with glutaraldehyde in 5-25% sucrose gradient and buffer exchanged to Tris buffer pH 7.5, 50 mM potassium acetate,0.5 mM TCEP.

JEOL 2010F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 0.5 2.0 60000.0 SIDE ENTRY, EUCENTRIC 298.0 GATAN ULTRASCAN 4000 (4k x 4k) COUNTING 1-48 1 364 1.0 30.0 1 RELION 2.1 CryoSparc C1 17.5 FSC 0.143 CUT-OFF cryoSPARC 3 2592 PROJECTION MATCHING cryoSPARC PROJECTION MATCHING cryoSPARC 4 cryoSPARC 4BKX 2VID RIGID BODY FIT