2024-12-04 REL PDBe PDBe PDBe 2020-01-18 2021-01-27 2021-01-27 2024-12-04 Israel Science Foundation 386/18 Israel Cin8 motor domain (short loop 8) bound to microtubules Siegler N Singh SK Zalk R Debs GE Sindelar CV Gheber L Zarivach R Kinesin, Cin8, Motor domain, Microtubules, MOTOR PROTEIN Singh SK Siegler N Pandey H Yanir N Popov M Goldstein-Levitin A Sadan M Debs G Zarivach R Frank GA Kass I Sindelar CV Zalk R Gheber L Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin. Sci Adv US 10 eadi1367 eadi1367 2024 38324691 doi:10.1126/sciadv.adi1367 2375-2548 ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN ALPHA and Beta TUBULIN WITH CIN8 MOTOR DOMAIN 0 1
ADDED 0.1 mM AMP-PNP, 10 micromolar Taxol
Saccharomyces cerevisiae Cin8, microtubules Saccharomyces cerevisiae Escherichia coli LEVO MPAENQNTGQDRSSNSISKNGNSQVGCHTVPNEELNITVAVRCRGRNEREISMKSSVVVNVPDITGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQDLIFDEVAGPLFQDFIKGYNCTVLVYGMTSTGKTYTMTGDEKLYNGELSDAAGIIPRVLLKLFDTLELQQNDYVVKCSFIELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFDNNNNNSSIYIQNLQEFHITNAMEGLNLLQKGLKHRQVASTKMNDFSSRSHTIFTITLYKKHQDELFRISKMNLVDLAGSENINRSGALNQRAKEAGSINQSLLTLGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTALIATISPAKVTSEETCSTLEYASKAKNIKNKPQLGSFIMKDILLEHHHHHH helical particle 6.9 Quantifoil R1.2/1.3 COPPER 300 CARBON HOLEY ETHANE HOMEMADE PLUNGER FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.3 GATAN K2 QUANTUM (4k x 4k) COUNTING 80.0 1 82.18 -0.53 C14 6.1 FSC 0.143 CUT-OFF 47551 47551 RELION 2.1
Number of particles seleced for reconstruction
 NOT APPLICABLE RELION 2.1 emd_10625.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 248 248 248 0 0 0 248 248 248 272.80002 272.80002 272.80002 90.0 90.0 90.0 X Y Z -0.081666864 0.29207584 -0.008126179 0.013407394 1.1 1.1 1.1 0.08 AUTHOR Cin8 motor domain (short loop 8) bound to microtubules