2024-05-22 REL PDBe PDBe PDBe 2020-01-15 2020-01-29 2020-01-29 2024-05-22 Max Planck Society Germany Subunits BBS 1,4,8,9,18 of the human BBSome complex Klink BU Raunser S ciliary transport, Arl6 effector, adaptor protein, complex, PROTEIN TRANSPORT Klink BU Gatsogiannis C Hofnagel O Wittinghofer A Raunser S Structure of the human BBSome core complex. Elife US 9 2020 31951201 doi:10.7554/eLife.53910 2050-084X EMD-10618 other EM volume
One additional subunit (BBS5) was detected in a reconstruction from a subset of particles
 6xt9 FULLOVERLAP BBSome core complex BBSome core complex 0 1 2 3 4 5
BBSome core complex containing BBS1,4, 8, 9 and 18. BBS5 was also present in the sample preparation, but was only visible in a subset of particles (see related entry)
Homo sapiens Bardet-Biedl syndrome 1 protein Homo sapiens 0.065159266 1 Trichoplusia ni LEVO MAAASSSDSDACGAESNEANSKWLDAHYDPMANIHTFSACLALADLHGDGEYKLVVGDLGPGGQQPRLKVLKGPLVMTES PLPALPAAAATFLMEQHEPRTPALALASGPCVYVYKNLRPYFKFSLPQLPPNPLEQDLWNQAKEDRIDPLTLKEMLESIR ETAEEPLSIQSLRFLQLELSEMEAFVNQHKSNSIKRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILA KMSLPSVPVFLEVSGQFDVEFRLAAACRNGNIYILRRDSKHPKYCIELSAQPVGLIRVHKVLVVGSTQDSLHGFTHKGKK LWTVQMPAAILTMNLLEQHSRGLQAVMAGLANGEVRIYRDKALLNVIHTPDAVTSLCFGRYGREDNTLIMTTRGGGLIIK ILKRTAVFVEGGSEVGPPPAQAMKLNVPRKTRLYVDQTLREREAGTAMHRAFQTDLYLLRLRAARAYLQALESSLSPLST TAREPLKLHAVVQGLGPTFKLTLHLQNTSTTRPVLGLLVCFLYNEALYSLPRAFFKVPLLVPGLNYPLETFVESLSNKGI SDIIKVLVLREGQSAPLLSAHVNMPGSEGLAAA Q8NFJ9 Bardet-Biedl syndrome 4 protein Homo sapiens 0.05946301999999999 1 Trichoplusia ni LEVO MHHHHHHGPMAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIY VQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQF NKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTY DPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLG LVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNLEDIENAKRAYAEAVHLDKCNPLVNLNYAVLLYNQGEKKNA LAQYQEMEKKVSLLKDNSSLEFDSEMVEMAQKLGAALQVGEALVWTKPVKDPKSKHQTTSTSKPASFQQPLGSNQALGQA MSSAAAYRTLPSGAGGTSQFTKPPSLPLEPEPAVESSPTETSEQIREK Q96RK4 Tetratricopeptide repeat domain 8 isoform 2 Homo sapiens 0.05870253899999999 1 Trichoplusia ni LEVO MDYKDDDDKAGPMSSEMEPLLLAWSYFRRRKFQLCADLCTQMLEKSPYDQAAWILKARALTEMVYIDEIDVDQEGIAEMM LDENAIAQVPRPGTSLKLPGTNQTGGPSQAVRPITQAGRPITGFLRPSTQSGRPGTMEQAIRTPRTAYTARPITSSSGRF VRLGTASMLTSPDGPFINLSRLNLTKYSQKPKLAKALFEYIFHHENDVKTALDLAALSTEHSQYKDWWWKVQIGKCYYRL GMYREAEKQFKSALKQQEMVDTFLYLAKVYVSLDQPVTALNLFKQGLDKFPGEVTLLCGIARIYEEMNNMSSAAEYYKEV LKQDNTHVEAIACIGSNHFYSDQPEIALRFYRRLLQMGIYNGQLFNNLGLCCFYAQQYDMTLTSFERALSLAENEEEAAD VWYNLGHVAVGIGDTNLAHQCFRLALVNNNNHAEAYNNLAVLEMRKGHVEQARALLQTASSLAPHMYEPHFNFATISDKI GDLQRSYVAAQKSEAAFPDHVDTQHLIKQLRQHFAML A0A0C4DGY3 Protein PTHB1 Homo sapiens 0.099383914 1 Trichoplusia ni LEVO MSLFKARDWWSTILGDKEEFDQGCLCLANVDNSGNGQDKIIVGSFMGYLRIFSPHPAKTGDGAQAEDLLLEVDLRDPVLQ VEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQCQMKLMYEHNLQRTACNMTYGSFGGVKGRDLICIQSMDGM LMVFEQESYAFGRFLPGFLLPGPLAYSSRTDSFLTVSSCQQVESYKYQVLAFATDADKRQETEQQKLGSGKRLVVDWTLN IGEQALDICIVSFNQSASSVFVLGERNFFCLKDNGQIRFMKKLDWSPSCFLPYCSVSEGTINTLIGNHNNMLHIYQDVTL KWATQLPHIPVAVRVGCLHDLKGVIVTLSDDGHLQCSYLGTDPSLFQAPNVQSRELNYDELDVEMKELQKIIKDVNKSQG VWPMTEREDDLNVSVVVSPNFDSVSQATDVEVGTDLVPSVTVKVTLQNRVILQKAKLSVYVQPPLELTCDQFTFEFMTPD LTRTVSFSVYLKRSYTPSELEGNAVVSYSRPTDRNPDGIPRVIQCKFRLPLKLICLPGQPSKTASHKITIDTNKSPVSLL SLFPGFASQSDDDQVNVMGFHFLGGARITVLASKTSQRYRIQSEQFEDLWLITNELILRLQEYFEKQGVKDFACSFSGSI PLQEYFELIDHHFELRINGEKLEELLSERAVQFRAIQRRLLARFKDKTPAPLQHLDTLLDGTYKQVIALADAVEENQGNL FQSFTRLKSATHLVILLIALWQKLSADQVAILEAAFLPLQEDTQELGWEETVDAAISHLLKTCLSKSSKEQALNLNSQLN IPKDTSQLKKHITLLCDRLSKGGRLCLSTDAAAPQTMVMPGGCTTIPESDLEERSVEQDSTELFTNHRHLTAETPRPEVS PLQGVSE Q3SYG4 BBSome-interacting protein 1 Homo sapiens 0.015430824 1 Trichoplusia ni LEVO MASWSHPQFEKGSAGSAAGSGAGWSHPQFEKGAGLEVLFQGPKRAEFMLKAAAKRPELSGKNTISNNSDMAEVKSMFREV LPKQGPLFVEDIMTMVLCKPKLLPLKSLTLEKLEKMHQAAQNTIRQQEMAEKDQRQITH A8MTZ0 singleParticle particle 0.08 7.5 20.0 TRIS-HCL 150.0 NaCl 0.1 TCEP UltrAuFoil GOLD 300 GLOW DISCHARGE AIR ETHANE 100 286 FEI VITROBOT MARK III
double blot with 2 minutes incubation after first sample application.
The sample was cross linked with 0.5% glutaraldehyde
 FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.3 1.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 1-50 15266 15.0 67.0 1 2831329 Initial models were generated de novo using RaptorX for most of the structure.
For building an atomic model of the beta propeller domains, we used the two available crystal structures of the beta propeller domains of C. reinhardtii BBS1 (PDB ID 4V0M) and of human BBS9 (PDB ID 4YD8) as starting points.
 C1 3.8 FSC 0.143 CUT-OFF SPHIRE 1.2-1.3 Meridien 862114 MAXIMUM LIKELIHOOD SPHIRE 1.2-1.3 MAXIMUM LIKELIHOOD SPHIRE 1.2-1.3 Meridien emd_10617.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 299.6 299.6 299.6 90.0 90.0 90.0 X Y Z -0.060557723 0.11145467 0.00021456317 0.002398998 1.07 1.07 1.07 0.015 AUTHOR emd_10617_msk_1.map emd_10617_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 299.6 299.6 299.6 90.0 90.0 90.0 X Y Z -0.016368514 0.037811298 -0.00006070093 0.0019044746 1.07 1.07 1.07 AUTHOR emd_10617_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 280 280 280 0 0 0 280 280 280 299.6 299.6 299.6 90.0 90.0 90.0 X Y Z -0.018108228 0.037507 -0.000248184 0.0018901974 1.07 1.07 1.07 AUTHOR