REL PDBe PDBe PDBe 2003-10-08 2003-10-08 2003-11-25 2011-05-26 The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Bhella D Goodfellow IG Roversi P Pettigrew D Chaudhry Y Evans DJ Lea SM Bhella D Goodfellow IG Roversi P Pettigrew D Chaudhry Y Evans DJ Lea SM The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). J.BIOL.CHEM. 279 8325 8332 2004 14634014 doi:10.1074/jbc.M311334200 1upn FULLOVERLAP Echovirus type 12 Echovirus type 12 1 Human echovirus 12 Human echovirus 12 Homo sapiens VERTEBRATES VIRION SEROTYPE false false EV12 singleParticle particle 0.2 7.4
PBS A
 NEGATIVE
Absorb protein to grid, float on 20% ammonium molybdate pH 7.4 for 10 seconds blot and plunge
400 mesh Quantifoils
 ETHANE blot for 2 seconds, wait for 2 seconds, plunge JEOL 1200EXII FLOOD BEAM BRIGHT FIELD LAB6 120 3.4 0.3 2.0 30000.0 29200.0 OTHER objective lens corrected at 200k x
MICROSCOPE JEOL 1200 EX with OXFORD INSTRUMENTS CRYO-TRANSFER STAGE
KODAK SO-163 FILM 3.42 28
Scanned with a Dunvegan HiScan
16. side entry
merge of focal pair images of single particles
 I OTHER 18.0 FSC 0.5 CUT-OFF EM3DR2, PFT2, CTFMIX
Particles were aligned using a model based strategy starting with a model derived from the crystallographic co-ordinates of EV-1, filtered to 16 Angstroms resolution. The program is called PFT (Polar Fourier Transform). The reconstructions were calculated using the EM3DR2 program which is based on the standard method of calculating icosahedral reconstructions as described by Crowther,'Fourier-Bessel'.
617 emd_1058.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 175 175 175 -87 -87 -87 175 175 175 598.5 598.5 598.5 90 90 90 X Y Z 0.0 255.0 72.036100000000005 25.678999999999998 3.42 3.42 3.42 149.0 Reconstruction of Echovirus type 12 from cryo-negative stain image data
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3D crystal structure fitting details lodged with PDB 1UPN