2024-05-22 REL PDBe PDBe PDBe 2019-12-27 2020-01-15 2020-01-15 2024-05-22 PKM2 in complex with L-threonine Saur M Hartshorn MJ PKM2, FBDD, L-threonine, CYTOSOLIC PROTEIN Saur M Hartshorn MJ Dong J Reeks J Bunkoczi G Jhoti H Williams PA Fragment-based drug discovery using cryo-EM. Drug Discov Today UK 25 485 490 2020 31877353 doi:10.1016/j.drudis.2019.12.006 1878-5832 EMD-10575 other EM volume
PKM2 in complex with Compound 5
 6tth FULLOVERLAP Pyruvate Kinase M2 Pyruvate Kinase M2 0 1 Homo sapiens 0.24 Pyruvate kinase PKM Homo sapiens 0.059831819999999994 4 Escherichia coli LEVO MGSSHHHHHHSSGLVPRGSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKE MIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKIT LDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSE KDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPA EKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAR EAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQA HLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP P14618 2.7.1.40 1,6-di-O-phosphono-beta-D-fructofuranose 0.00034011599999999995 4 FBP THREONINE 0.000119119 4 THR water 1.8015e-05 192 HOH singleParticle particle 0.16 8.199999999999999 Quantifoil R1.2/1.3 COPPER 300 GRAPHENE OXIDE CONTINUOUS ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) COUNTING 1 476 59.98 66.8 1 190577
in-house X-ray structure
 D2 2.6 FSC 0.143 CUT-OFF RELION 3.04 99837 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_10576.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 171 222 258 0 0 0 171 222 258 85.5 111.0 129.0 90.0 90.0 90.0 X Y Z -0.20393747 0.40100566 0.003720325 0.027403597 0.5 0.5 0.5 0.06 AUTHOR None emd_10576_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 358 358 358 0 0 0 358 358 358 356.32098 356.32098 356.32098 90.0 90.0 90.0 X Y Z -0.16106024 0.29889014 -0.0000024363856 0.006778423 0.99531 0.99531 0.99531 AUTHOR Relion post-process unmasked map from halfmaps which have not been resampled after auto-refine. emd_10576_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 86 112 130 0 0 0 86 112 130 85.596664 111.474724 129.3903 90.0 90.0 90.0 X Y Z -0.16106024 0.29889014 0.0036978228 0.02456755 0.99531 0.99531 0.99531 AUTHOR None emd_10576_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 358 358 358 0 0 0 358 358 358 356.32098 356.32098 356.32098 90.0 90.0 90.0 X Y Z -0.05120274 0.12864439 -0.0000023370758 0.003281319 0.99531 0.99531 0.99531 AUTHOR Relion auto-refine halfmap 2 emd_10576_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 358 358 358 0 0 0 358 358 358 356.32098 356.32098 356.32098 90.0 90.0 90.0 X Y Z -0.050882563 0.14051148 -0.0000025356953 0.0032726792 0.99531 0.99531 0.99531 AUTHOR Relion auto-refine halfmap 1