REL PDBe PDBe PDBe 2003-10-08 2003-10-08 2003-11-25 2011-05-26 The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Bhella D Goodfellow IG Roversi P Pettigrew D Chaudhry Y Evans DJ Lea SM Bhella D Goodfellow IG Roversi P Pettigrew D Chaudhry Y Evans DJ Lea SM The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). J.BIOL.CHEM. 279 8325 8332 2004 14634014 doi:10.1074/jbc.M311334200 1upn FULLOVERLAP Echovirus type 12 bound to decay accelerating factor domains 3 and 4 Echovirus type 12 bound to decay accelerating factor domains 3 and 4 2 Human echovirus 12 Human echovirus 12 Homo sapiens VERTEBRATES VIRION SEROTYPE false false EV12 Decay accelerating factor domains 3 and 4 60 Monomer true Homo sapiens Human Komagataella pastoris singleParticle particle 0.2 7.4
PBS A
 NEGATIVE
Protein absorbed to grid, floated onto 20% ammonium molybdate (pH 7.4), blotted and plunged into liquid ethane
400 mesh Quantifoils
 ETHANE blot for 2 seconds, wait for 2 seconds plunge JEOL 1200EXII FLOOD BEAM BRIGHT FIELD LAB6 120 3.4 0.3 2.0 30000.0 29200.0 OTHER objective astigmatism corrected at 200k x
MICROSCOPE JEOL 1200 EX with OXFORD INSTRUMENTS CRYO-TRANSFER STAGE
KODAK SO-163 FILM OTHER 3.42 16
Images scanned on a Dunvegan HiScan
16. side entry
Merged individual particles from focal pairs
 I OTHER 16.0 FSC 0.5 CUT-OFF EM3DR2, PFT2, CTFMIX
Particles were aligned using a model based strategy starting with a model derived from the crystallographic co-ordinates of EV-1, filtered to 16 Angstroms resolution. The program is called PFT (Polar Fourier Transform). The reconstructions were calculated using the EM3DR2 program which is based on the standard method of calculating icosahedral reconstructions as described by Crowther,'Fourier-Bessel'.
903 emd_1057.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 175 175 175 -87 -87 -87 175 175 175 598.5 598.5 598.5 90 90 90 X Y Z 0.0 255.0 66.186300000000003 31.677700000000002 3.42 3.42 3.42 169.0 Three dimensional reconstruction of echovirus type 12 bound to domains three and four of its cellular receptor decay-accelerating factor. Calculated from cryo-negative stain images
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3D crystal structure fitting details lodged with PDB 1UPN