REL PDBe PDBe PDBe 2003-09-22 2003-09-29 2004-01-06 2013-12-04 Locking and unlocking of ribosomal motions. Valle M Zavialov A Li W Stagg SM Sengupta J Nielsen RC Nissen P Hervey SC Ehrenberg M Frank J Valle M Zavialov A Li W Stagg SM Sengupta J Nielsen RC Nissen P Harvey SC Ehrenberg M Frank J Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy. NAT.STRUCT.MOL.BIOL. 10 899 906 2003 14566331 doi:10.1038/nsb1003 1qza FULLOVERLAP 1qzd FULLOVERLAP 1r2x FULLOVERLAP 70S ribosome from E. coli complex 70S-fMet-tRNA-Phe-tRNA-EF-Tu-GDP-kirromycin. 70S ribosome from E. coli complex 70S-fMet-tRNA-Phe-tRNA-EF-Tu-GDP-kirromycin. 5 70S from E. coli false Escherichia coli ribosome-prokaryote: ALL fMet-tRNAfMet Escherichia coli OTHER DOUBLE HELIX false Phe-tRNAPhe Escherichia coli OTHER DOUBLE HELIX false Elongation Factor Tu Escherichia coli 1 false deacylated tRNA Escherichia coli OTHER DOUBLE HELIX false singleParticle particle 7.5
5 mM potassium phosphate, 5 mM magnesium acetate, 5 mM ammonium chloride, 95 mM potassium chloride, 0.5 mM calcium chloride, 8 mM putrescine, 1 mM spermidine, and 1 mM dithioerythritol
 NEGATIVE
Cryo-electron microscopy. No stain.
300 mesh Quantifoil R2/4
 ETHANE 90 two-face blotting for 1 second FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 2.0 4.0 50000.0 49650.0 GATAN LIQUID NITROGEN 90 corrected at 175,000 KODAK SO-163 FILM ZEISS SCAI 14 50 15 1.0 16. single tilt cryoholder 0 0
segregation in defocus groups and correction in volumes
 C1 OTHER 9.0 OTHER Spider 75996 emd_1055.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 -65 -65 -65 130 130 130 366.6 366.6 366.6 90 90 90 X Y Z -85.858900000000006 250.984000000000009 4.44442 25.344000000000001 2.82 2.82 2.82 57.200000000000003 70S + fMet-tRNA + PhetRNA + EF-Tu + GDP + kir mRNA codes for MP-stop
::::EMDATABANK.org::::EMD-1055::::
 1FNM 1FFK 1FJF RIGID BODY FIT O
Protocol: rigid body. Manual fitting in O.The docking of X-ray structures into the cryo-EM maps was made using O and the visualization was performed in IRIS Explorer. EF-G domains were taken from the Thermus thermophilus H573A mutant crystal structure in complex with GDP (PDB code: 1FNM). The coordinates of the ribosomal proteins and rRNAs were taken from published atomic structures (PDB code 1FFK for the 50S subunit; 1FJF for the 30S subunit and the docking was performed using the coordinates as rigid bodies.
correlation coeficient