2024-05-22 REL PDBe PDBe PDBe 2019-12-05 2019-12-18 2020-04-22 2024-05-22 Structure of the chaperonin gp146 from the bacteriophage EL (Pseudomonas aeruginosa) in the apo state Bracher A Wang H molecular chaperone, ATPase, chaperonin, CHAPERONE Bracher A Paul SS Wang H Wischnewski N Hartl FU Hayer-Hartl M Structure and conformational cycle of a bacteriophage-encoded chaperonin. Plos One US 15 e0230090 e0230090 2020 32339190 doi:10.1371/journal.pone.0230090 1932-6203 6tmv FULLOVERLAP The chaperonin gp146 from the bacteriophage EL (Pseudomonas aeruginosa) in the apo state The chaperonin gp146 from the bacteriophage EL (Pseudomonas aeruginosa) in the apo state 0 1 Pseudomonas phage EL 0.864 Putative GroEL-like chaperonine protein Pseudomonas phage EL 0.06176094099999999 14 Escherichia coli BL21(DE3) LEVO MSQTLLVHGKDAQGIIKQVLSEVYDAVTSTMGPNGQLVMIKNGVSTKTTKDGVTVARSIRFADEAHELVNRVITEPATKT DEECGDGTTTTIMLTHALYHLFKDFPGFQHHRNIEDLVERVIQRLESMAIRVEVDDPRLYQVALTSSNQDEKLARLVSEL YANNKGSYPDIELKEGVNFEDQIEQTTGRTIRMFYANPWFAKGHQGGVTELTGFTAFVIDRRIDKEDTQKLIDGVNHLVK THKQHLALPILLIARSFEEAANSTLMQLNAAHPTLVEDGRPWLIPLSTPVGGAIGTSELQDIAVMLNAPMLSDVADLTKL DTHSINGQHGQLELGGNRSILKSTTPKDEDRIEQHARGIEELLEGFSLSDKFSVRARYNERRIRTLRGKLITISVGGETY SEVKERVDRYEDVVKAIRSALENGILPGGGVSLVKAVFGTIKEGLEDKDQSAEFAKRYINSGIANELMRLSTIQHKLLFK DTALYKENGSFHFNDDWLNTPTVMNLATGEIGTPEGLGIYDTAYASITALKGGLQTAKILATTKTLILGEKLSAVKVR Q2Z0T5 singleParticle particle 1.125 7.5 20.0 C8H18N2O4S HEPES 50.0 NaCl sodium chloride 0.5 C10H16N2O8 EDTA 4.0 MgCl2 magnesium chloride Quantifoil R2/1 COPPER 300 CARBON HOLEY GLOW DISCHARGE AIR ETHANE 100 298 FEI VITROBOT MARK IV
Blot time was 2 sec..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K3 (6k x 4k) 1 2519 0.11984 1.552 GATAN K3 (6k x 4k) 1 3063 0.12 1.55 1 690973 1 C2 3.45 FSC 0.143 CUT-OFF RELION 3.0 178107 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 6 59363.0 RELION 3.0 emd_10528.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 294 294 294 0 0 0 294 294 294 320.46002 320.46002 320.46002 90.0 90.0 90.0 X Y Z -0.10070717 0.18211088 0.0007228104 0.005765548 1.09 1.09 1.09 0.025 AUTHOR CryoEM map of apo gp146, the chaperonin from bacteriophage EL (Pseudomonas putida) RIGID BODY FIT
Jelly body refinement C2 symmetry NCS restraints
REAL