REL PDBe PDBe PDBe 2003-08-28 2003-08-28 2003-08-28 2011-05-26 Untangling desmosomal knots with electron tomography. He W Cowin P Stokes DL He W Cowin P Stokes DL Untangling desmosomal knots with electron tomography. SCIENCE 302 109 113 2003 14526082 doi:10.1126/science.1086957 1q55 FULLOVERLAP 1q5a FULLOVERLAP 1q5b FULLOVERLAP 1q5c FULLOVERLAP mouse skin mouse skin
Skin from newborn mice frozen by high-pressure freezer followed by freeze-substitution, embedding in Epon resin and thin sectioning.
desmosome 1 desmosome
in-situ desmosome from frozen skin
1 unique false Mus musculus mouse mouse skin cell junction between two cells plasma membrane tomography 7.4
0.5mM MgCl2 and 1-2mM CaCl2 in PBS
 NEGATIVE
staining en bloc freeze-substitution with 1% OsO4/0.1% uranyl acetate in actone, thin section stained with 3% uranyl acetate/SATO Pb post-stain
200 mesh thin bar hexgonal cooper grid with formvar
 NITROGEN 100 90 OTHER
Vitrification instrument: BalTec HPM 010. high pressure freezing at 2050 bar with liquid nitrogen
50 High pressure freezing FEI/PHILIPS CM200FEG FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 0.3 0.5 38000.0 OTHER 293 293 293 objective lens astigmatism was corrected with thin carbon at 200-390kx
dose corresponds to the cumulative dose for the entire dataset.
2002-09-17 GATAN MULTISCAN
imaging directly on the CCD
 high tilt 1
50nm thin section stained with 3% uranyl acetate and followed by SATO lead stain, picked on formvar coated grids and both sides coated 5-10nm amorphous carbon.
OTHER 30.0 OTHER IMOD
tomographic reconstruction from dual axis tilt series (tilt range:-58/+53; -62/+57, interval:1 degree). reconstruction from -62/+57 tilt series.
150
no CTF correction
 emd_1051.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 512 512 52 0 0 0 512 512 52 4765.7 4765.7 484.016 90 90 90 X Y Z -5609.0 6038.0 1381.049999999999955 380.76400000000001 9.308 9.308 9.308 1950.0 This is a 3D reconstruction map of an desmosome based on high pressure freezing/freeze-subsitution electron tomography
::::EMDATABANK.org::::EMD-1051::::
 1L3W AmiraMol 3.0
This map has not been used for fitting with C-cadherin molecules; An different desmosome tomographic map in the same sample(EMD entry 1052) was used for fitting C-cadherin structure.Results deposited in PDB database under codes 1Q55, 1Q5A, 1Q5B and 1Q5C