2024-05-22 REL PDBe PDBe PDBe 2019-11-17 2020-01-29 2020-02-19 2024-05-22 European Commission PIEF-GA-2012-331285 Germany European Commission PCOFUND-GA-2008-229597 Germany German Research Foundation EXC1074 Germany Cryo-EM structure of AtNBR1-PB1 filament (S-type) Jakobi AJ Sachse C Autophagy, helical filament, SIGNALING PROTEIN Jakobi AJ Huber ST Mortensen SA Schultz SW Palara A Kuhm T Shrestha BK Lamark T Hagen WJH Wilmanns M Johansen T Brech A Sachse C Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake. Nat Commun UK 11 440 440 2020 31974402 doi:10.1038/s41467-020-14343-8 2041-1723 6tgp FULLOVERLAP AtNBR1-PB1 (S-type) AtNBR1-PB1 (S-type) 0 1
Helical filament of AtNBR1-PB1 domain (1-95)
Arabidopsis thaliana Protein NBR1 homolog Arabidopsis thaliana 0.010198557 2 Escherichia coli BL21(DE3) LEVO MESTANALVVKVSYGGVLRRFRVPVKANGQLDLEMAGLKEKIAALFNLSADAELSLTYSDEDGDVVALVDDNDLFDVTNQ RLKFLKINVNAGVS Q9SB64 helical filament 0.4 7.5 50.0 HEPES 150.0 NaCl Sodium chloride 0.05 TCEP C-flat-1.2/1.3 4C COPPER 400 CARBON HOLEY GLOW DISCHARGE ETHANE 100 288 LEICA EM GP FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 4.5 59000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON II (4k x 4k) INTEGRATING 684 14.0 1 5.905 -31.17 C1 4.4 FSC 0.143 CUT-OFF SPRING 0.85 18021 NOT APPLICABLE SPRING 0.85 emd_10500.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 140 0 0 0 100 100 140 138.6 138.6 194.04001 90.0 90.0 90.0 X Y Z -2.8809712 3.9452217 0.000000001985075 0.99999964 1.386 1.386 1.386 2.1 AUTHOR Globally sharpened map FLEXIBLE FIT Correlation coefficient REAL