REL PDBe PDBe PDBe 2003-05-29 2003-05-19 2003-06-03 2012-10-24 A cellular receptor of human rhinovirus type 2, the very-low-density lipoprotein receptor, binds to two neighboring proteins of the viral capsid. Neumann E Moser R Snyers L Blaas D Hewat EA Neumann E Moser R Snyers L Blaas D Hewat EA A cellular receptor of human rhinovirus type 2, the very-low-density lipoprotein receptor, binds to two neighboring proteins of the viral capsid. J.VIROL. 77 8504 8511 2003 12857919 doi:10.1128/JVI.77.15.8504-8511.2003 Human Rhinovirus 2 in complex with its cellular receptor, VLDL-R Human Rhinovirus 2 in complex with its cellular receptor, VLDL-R 2 Human rhinovirus 2 Human rhinovirus 2 Homo sapiens VERTEBRATES 300 VIRION SEROTYPE false false Cellular Receptor
The VLDL-R consists of eight imperfect ligand-binding repeats of approximately 40 amino acids at its N-terminus. Recombinant VLDL-minireceptors encompassing different ligand-binding repeats are here expressed with the first three repeats + a Maltose Binding Protein fused to the N-terminus + a Hexa-his-tag fused to the C-terminus.
true Homo sapiens Human muscle Escherichia coli pMALTM-c2x singleParticle particle 0.82 7.4
50 mM Tris-HCl for the virus 2 mM CaCl2, 20 mM Tris-HCL for the receptor fragment
300 mesh copper grid
 ETHANE Blot with filter paper for 1-2 seconds JEOL 2010F SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 200 1.4 1.18 2.9 40000.0 39700.0 OTHER objective lens astigmatism was corrected at 300,000 times magnification KODAK SO-163 FILM OTHER 7 33 15
Zeiss PhotoScan TD scanner
 Oxford cryo-holder CT3200
HRV2 and receptor fragment were incubated for 1 hour at 4 degree Celcius.
CTFMIX
 I OTHER 16.0 FSC 0.5 CUT-OFF
Methods for reconstructing density maps of "single" particles from cryoelectron micrographs to subnanometer resolution (Conway et al.,J Struct Biol. 1999 Dec 1;128(1):106-18.)
912 emd_1049.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 255 255 255 -127 -127 -127 255 255 255 448.8 448.8 448.8 90.0 90.0 90.0 X Y Z -17360.0 25708.0 312.54598999000001 3491.530029300000024 1.76 1.76 1.76 4380.0 AUTHOR This is a 3D reconstruction of HRV2/MBP-VLDLR(-123)
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 RIGID BODY FIT O
Protocol: Manual docking. Fitting the X-RAY structures of HRV2 and the VLDL-R repeats to the cryo-electron microscope reconstructed density. Determination of the residues included in the footprints.
REAL