Three-dimensional structure of bacteriophage T4 baseplate.

REL PDBe PDBe PDBe 2003-05-19 2003-05-19 2003-09-19 2012-10-24 Three-dimensional structure of bacteriophage T4 baseplate. Kostyuchenko VA Leiman PG Chipman PR Kanamaru S vanRaaij MJ Arisaka F Mesyanzhinov VV Rossmann MG Kostyuchenko VA Leiman PG Chipman PR Kanamaru S van Raaij MJ Arisaka F Mesyanzhinov VV Rossmann MG Three-dimensional structure of bacteriophage T4 baseplate. NAT.STRUCT.BIOL. 10 688 693 2003 12923574 doi:10.1038/nsb970 1pdf FULLOVERLAP 1pdi FULLOVERLAP 1pdj FULLOVERLAP 1pdl FULLOVERLAP 1pdm FULLOVERLAP 1pdp FULLOVERLAP 2fl8 FULLOVERLAP 3h3w FULLOVERLAP Bacteriophage T4 baseplate-tail tube assembly Bacteriophage T4 baseplate-tail tube assembly the complex has sixfold symmetry 1 baseplate-tail tube complex Enterobacteria phage T4 T4 18-,23- bacteriophage T4 baseplate and tail tube true Escherichia coli BE singleParticle particle 5 7.0

water

holey carbon

ETHANE Blot for 2 seconds before plunging FEI/PHILIPS CM300FEG/T FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2 1.2 5.0 45000.0 47000.0 GATAN LIQUID NITROGEN 2001-01-30 KODAK SO-163 FILM ZEISS SCAI 14 15 25

images were scanned at 7 micron per pixel and averaged 2x2 to give 14 micron per pixel

8. 626 Single Tilt Cryotransfer System 0 0

each particle

C6 OTHER 12.0 FSC 0.5 CUT-OFF SPIDER

modified SPIDER version was used to allow reconstruction of the whole baseplate-tail tube assembly

945 emd_1048.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 196 196 196 -98 -98 -97 196 196 196 583.8291 583.8291 583.8291 90.0 90.0 90.0 X Y Z -7.64827013 10.344811440000001 0.0 1.0 2.97872 2.97872 2.97872 1.45 AUTHOR map contains the baseplate and the proximal part of the tail tube

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RIGID BODY FIT Situs 2.0, Colores