2024-11-20 REL PDBe PDBe PDBe 2019-11-10 2019-11-27 2019-11-27 2024-11-20 Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, membrane region Muhleip A Amunts A mitochondria, ATP synthase, MEMBRANE PROTEIN Muhleip A McComas SE Amunts A Structure of a mitochondrial ATP synthase with bound native cardiolipin. Elife US 8 2019 31738165 doi:10.7554/eLife.51179 2050-084X 6tdv FULLOVERLAP Euglena gracilis mitochondrial ATP synthase dimer Euglena gracilis mitochondrial ATP synthase dimer 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Euglena gracilis 2 ATPTB1 Euglena gracilis 0.056026699 2 LEVO MTAVVKNLAKLPAATSQILTNVSKLQTFHGLLEERRDKYAPLAYHTYDNLKQKTTWHPIAHAWVDEGLPVSKKEYNEYCW LKKDMQRLLPLASPFVFGIYGILPLAVWLSNDGYLPSAFSSKKDIVSKKLEWYSSYGDDLRQQVGPMLQHRLKRHLRGTL NNEHRLMLDEVTESYKEIFYSHYTGQLRDVRKCAHLRLYDGTSTVLLLTNKEPVELTSELLQKWNAIKAAKLSPEEEKKA RNEALIEAYKEQELHGGPHVKHMQGYGIPADTPLLGENAKGDQYTQPPESASIPLEQLEWTGDTVFIPAEYRTEMEDWGR ELTKLANQFLLLPWRFVSNAWNQRRLVSWFEEILQEDALIAKEGGVQALSDDELKVALLDRAVIRCDEELTRGDMEARYK EISWLMSLRNPFIVLAWQTGYYRSTYSPEDDLPEASILPKLNRTVLDVDVHNELAPDHPEKPLPRVHPALYPNSHLALAK EVAVLAK ATPTB3 Euglena gracilis 0.035175836 2 LEVO MAAKTIPFVASTALGERLFAGVQKVLAAAKAPVSLVQTDAKFAPADAKYLLAEPLSAAATGELANKYGLSSKVTSGAASQ FYPNSIYPSLNVEVVQNLYALSNPAFSSLSATTTKAVTVKDESAIKLAELQKETERNISSFFRDEANKSVQATLRLACDK AIKTKADKKTVMVVTKPHGDAFDDLLAQVTKSESDGRADELRNSSVSVEPTLVGNAWPKLVMFPEGVNVVVCGPNASGDQ VAQLFVGIAGGTGMVAQQLVGDAVVFTSANAEDNENPTGALLAASNLLTALGHEAEAKKIAAAVAKAYTTDRILPKELPG GKADLEAFIDAVAKHASA ATPTB6 Euglena gracilis 0.021737113 2 LEVO MTHAELHLFDLDEFMQTYKRLQTRQDWLIENKCKKSRLFSYVAAVIAFTVGKSATMSDEAILAKIDPYVTSEVRVQRGAW WRSGYFTKEEVEMMTPKGPIARYYKFLLGVRRFPLKHGALSWACGFVPAWLTFTSLNHWAQNRRLNRYLTQESVFGEMAR ELVRGKTADEATTSVMARVEKEILGVH ATPTB12 Euglena gracilis 0.011432077999999998 2 LEVO MSSYTGAALAPKSERLRLAFEEKQKDHQKCIEEAKGKGLKKDELIDACAWTHRKTILALKDWFAYRPPFQDRRSKWAEYC SIRHDSGSWLGWSQKFF subunit a Euglena gracilis 0.032666373 2 LEVO MLNSNIYIIIYGGIIMYSIMIIIQMFLYNFSNKIYIEVEINKYILSKNNIDIYWIICNCTIIIIITTLNHIINKIGIYNM IEYNICYWLIGTGLGLYISPFIVFGYKFFVYIMDLNNYSLNIYHNNNKMNDIQQIYNGTNYNDTMIFFIKDINNIFTIYR SINFFMNWLYQMIYYGVRMWLVFVLHSFSLGSFGELITVITDNNLIFNVFYIGLLGLGFILYLIVIFYLGIQIYVYISFS LSFLHSTILLFLVNYIPHYNNKSIFNTFTNKSIY subunit b Euglena gracilis 0.012673109 2 LEVO MPSTSPADKDVPMSILHTHGLSYVNWCMSLAPGLLVFEGFFRARYYRSRVPPSRTVLMNGLKMRMFSLARQQAPKIVHKP VLSPIPEHLRLVKNVAQVQIDMLKLLNAQAAK subunit d Euglena gracilis 0.053928289 2 LEVO MMRRACRIIRPSHVRGVSGVAPTIYLRSKAALPATSTTDVRPQLYALQRFAKAQLKTATEAERAAIEADIARYQEYLDSD LEKLKQDVAEDTAKKQKLIPLLDRYPDVPIEKIPEHANVLLKKIDACLEILSKDIGEVTDAEAHEMYFETSKFQILHIYT GCVASFPEGDVPPGAVECLPGQVIRTKVNGEDVMLEIDEVDPGYQVCWFKPDVPLPENAEILWSYPYEPTAALPTGTTWE EGQANVLIPAEPTPEAAVWPPTPVTNVYAPMAEKLALKSNPELKVLFKEALLQPAKLLPLDVDYQCSHDREVVEAKRDRY LTALVEAEQAPPLPFTPDVLQLQLEHNVLKGELIDRLRALEYTIVTEQLQARLHERRLRGDVIDEWEELDYHPLVRDDTY LAIDFGDPTFGRYIWKLFPHTDGDEECMFKDTRLDVLPPQVNPLNAILAQHTAQTPVHRSLEKRLWTEVRATAVSE subunit f Euglena gracilis 0.011193877 2 LEVO MAPLYPVLSQASLYKRHFFKNIKLFHVVFYVGAPCVTFGTAAWSGSNRNSREAIFMVIEERHGWDNFKKLSSHQQGVIMQ EAAQESLLARNKGELHLP subunit i/j Euglena gracilis 0.012529404 2 LEVO MVYTNWQSSYTRLFVSKPWMWHPLAWMTLSVGIWWKFGKESLCNERSFYIHTHPKWAPHKFHTVYNWSRDPIKWTLAEQY ASIIRNTNTDIEAVLKIKLPANAN subunit k Euglena gracilis 0.012739870999999998 2 LEVO MAFGRTRPTLSSPLVPVWNDLRALQVFTSQEYMQKRGPGFTNTLEYKLSCLNPVKWYDMMKVMPGGKAFVGTALGLALFG GWGVEFVKNISVMTKEKPPIDWNNEKLGHLTRS subunit 8 Euglena gracilis 0.007011208 2 LEVO LIPVSLVDLININIIFYILLLYTLLLFFIPLFLASINYTYHYIYKYYNYNYNFINNN ATPEG1 Euglena gracilis 0.019738803 2 LEVO MSLAKVWMYASWIPRGIPKAMANELSSAAAALAHPEAIARVAQLESQGKNPYRVARAEFWQMYLACWPYRFRNTVVEWET CKAKVLKGSVDLQDIVDLLYLLAWAYLFWILGEIYGRGSLYGYRFDGEIHRQEAQNVILYKEKEAQEMAVVMEKLEKEIQ EWLKTMEQE ATPEG2 Euglena gracilis 0.016210519 2 LEVO MPLPNAVVQGYTSVRGPKRPLDHFYGRTPLNIDTLWHWVKFPHRYDNLRFAVCFWAFLVSAHFANKKQRNLRVEWEKNME IQKKLHPSGLWSEEQAFAAAEKLGRPKAGHPMRVFEDGYQQFDLKPKLFDPDEEAHH ATPEG3 Euglena gracilis 0.013807529 2 LEVO MADHNKKDVGSWASPNEHLMFFDFSSWLLVDFGKRWERWVSFKKSFLTTTRSPYWSPQFFLLTFFQLRNSNVKLCENWNW APKGDDFNLLHNSAAEPFGRDLKAHLEREAGAKHHH ATPEG4 Euglena gracilis 0.013703778 2 LEVO MGGDAHAAPAEKPDPALDATKALPKALEEVEFFQSYAVRRKTGFHLFNRATGSPTIVGPMFYNLYNFVRIGRVSKYVCWL SLPLVFQRMWMKNRATGMEYDIDLENYAPFEAKKNPMHGH ATPEG5 Euglena gracilis 0.010825277 2 LEVO MSLPTFFLAPPLTEPFRARHRRKCIEKKDTFFQCLDKNKNETEPCEVEASGYSQECPDSWRRYFNEQRWKALELKGEVKH ANLKNTQQHF ATPEG6 Euglena gracilis 0.009236822 2 LEVO MFGVTRKLLGELSEYVEVNEKGMPKPQALSLWNMPYAKRRALTKFARGVRWQFIVLFIALYNFKNRDDSHLLRRGAYN ATPEG7 Euglena gracilis 0.008861477 2 LEVO MMRISRKLLVPVANFRPKKPWDGPWGIQISQKKDRPFIAMWILFPLLLVDHLTREYYAYWHSSKVPVTDVFGDF ATPEG8 Euglena gracilis 0.0074417879999999995 2 LEVO MGGKASEAVTIAFRFPHRTTFLVKQNVGQKLNKGHQTFWQLVAGGWLFFLLINRTSFKPKLAAPKV CARDIOLIPIN 0.0014640429999999997 25 CDL DODECYL-BETA-D-MALTOSIDE 0.000510615 8 LMT 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE 0.000648891 12 LPP FRAGMENT OF TRITON X-100 0.00035250799999999997 16 TRT singleParticle particle 7.4 ETHANE 100 277 FEI VITROBOT MARK IV
3 seconds blot.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 QUANTUM (4k x 4k) COUNTING 9045 10.0 36.3 1 555269 EMD-3559 C2 FOURIER SPACE 2.8 FSC 0.143 CUT-OFF RELION 150242 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION RELION emd_10468.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 461.99997 461.99997 461.99997 90.0 90.0 90.0 X Y Z -0.060570776 0.13339347 0.00020352937 0.0033610577 1.05 1.05 1.05 0.025 AUTHOR emd_10468_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 461.99997 461.99997 461.99997 90.0 90.0 90.0 X Y Z -0.017460573 0.058702648 0.00018340845 0.0021347709 1.05 1.05 1.05 AUTHOR emd_10468_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 461.99997 461.99997 461.99997 90.0 90.0 90.0 X Y Z -0.019038655 0.05732215 0.00022360627 0.0021333976 1.05 1.05 1.05 AUTHOR